Multi-parametric imaging of cell heterogeneity in apoptosis analysis

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dc.contributor.authorVorobjev, Ivan A.
dc.contributor.authorBarteneva, Natasha S.
dc.creatorIvan A., Vorobjev
dc.date.accessioned2017-12-22T08:55:31Z
dc.date.available2017-12-22T08:55:31Z
dc.date.issued2017-01-01
dc.description.abstractAbstract Apoptosis is a multistep process of programmed cell death where different morphological and molecular events occur simultaneously and/or consequently. Recent progress in programmed cell death analysis uncovered large heterogeneity in response of individual cells to the apoptotic stimuli. Analysis of the complex and dynamic process of apoptosis requires a capacity to quantitate multiparametric data obtained from multicolor labeling and/or fluorescent reporters of live cells in conjunction with morphological analysis. Modern methods of multiparametric apoptosis study include but are not limited to fluorescent microscopy, flow cytometry and imaging flow cytometry.In the current review we discuss the image-based evaluation of apoptosis on the single-cell and population level by imaging flow cytometry in parallel with other techniques. The advantage of imaging flow cytometry is its ability to interrogate multiparametric morphometric and fluorescence quantitative data in statistically robust manner. Here we describe the current status and future perspectives of this emerging field, as well as some challenges and limitations. We also highlight a number of assays and multicolor labeling probes, utilizing both microscopy and different variants of imaging cytometry, including commonly based assays and novel developments in the field.en_US
dc.identifierDOI:10.1016/j.ymeth.2016.07.003
dc.identifier.citationIvan A. Vorobjev, Natasha S. Barteneva, Multi-parametric imaging of cell heterogeneity in apoptosis analysis, In Methods, Volume 112, 2017, Pages 105-123en_US
dc.identifier.issn10462023
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S1046202316302055
dc.identifier.urihttp://nur.nu.edu.kz/handle/123456789/3053
dc.language.isoenen_US
dc.publisherMethodsen_US
dc.relation.ispartofMethods
dc.rights.license© 2016 Published by Elsevier Inc.
dc.subjectApoptosisen_US
dc.subjectFluorescent microscopyen_US
dc.subjectImaging flow cytometryen_US
dc.subjectFlow cytometryen_US
dc.subjectProgrammed cell deathen_US
dc.subjectMulticolor imagingen_US
dc.titleMulti-parametric imaging of cell heterogeneity in apoptosis analysisen_US
dc.typeArticleen_US
elsevier.aggregationtypeJournal
elsevier.coverdate2017-01-01
elsevier.coverdisplaydate1 January 2017
elsevier.endingpage123
elsevier.identifier.doi10.1016/j.ymeth.2016.07.003
elsevier.identifier.eid1-s2.0-S1046202316302055
elsevier.identifier.piiS1046-2023(16)30205-5
elsevier.identifier.scopusid84996618716
elsevier.issue.nameFlow Cytometry
elsevier.openaccess0
elsevier.openaccessarticlefalse
elsevier.openarchivearticlefalse
elsevier.startingpage105
elsevier.teaserApoptosis is a multistep process of programmed cell death where different morphological and molecular events occur simultaneously and/or consequently. Recent progress in programmed cell death analysis...
elsevier.volume112
workflow.import.sourcescience

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