Application of molecular genotyping to determine prevalence of HPV strains in Pap smears of Kazakhstan women

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dc.contributor.authorNiyazmetova, Luiza
dc.contributor.authorAimagambetova, Gulzhanat
dc.contributor.authorStambekova, Nazigul
dc.contributor.authorAbugalieva, Zaurech
dc.contributor.authorSeksembayeva, Korlukain
dc.contributor.authorSyed, Ali
dc.contributor.authorAzizan, Azliyati
dc.date.accessioned2017-11-08T10:57:13Z
dc.date.available2017-11-08T10:57:13Z
dc.date.issued2016-11-20
dc.description.abstractObjectives: Human papillomavirus is themain causative agent for cervical cancer. However, few data are available about HPV prevalence in Kazakhstan. The aims of this study were to genotype HPV DNA in Pap smear samples of women to determine prevalence of carcinogenic HPV types in Astana, Kazakhstan and to analyze the association between HPV positivity and the cytology results of patient samples. Methods: Pap smear materials were obtained from 140 patients aged 18-59, who visited the outpatient gynecological clinic. Microscopic examination was done to detect dysplasia, and HPV genotyping was done using real-time multiplex PCR. Results: HPV testing showed that among 61 HPV positive patients, the most prevalent types were 16 and 18. Microscopic examination showed that 79% of the samples had normal cytology, while 13% had CIN grade I, 5% had CIN grade II, and 3% had CIN grade III. The analysis revealed that 12% of the samples had CIN cytology and presence of HPV. Approximately 31% had HPV without cervical dysplasia, while 8% of samples were CIN positive without HPV infection. A statistically significant relationship between HPV 16 and HPV 33 positive samples and CIN grade II and III was found. Conclusions: Overall, this study will help to strengthen and guide health policy implementation of primary and secondary cervical cancer prevention strategies in Kazakhstan.2016 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync nd/4.0/).ru_RU
dc.identifier.citationNiyazmetova Luiza et al.(>6), 2016(November 20), Application of molecular genotyping to determine prevalence of HPV strains in Pap smears of Kazakhstan women, International Journal of Infectious Diseases, 85-88ppru_RU
dc.identifier.issn1201-9712
dc.identifier.urihttp://dx.doi.org/10.1016/j.ijid.2016.11.410
dc.identifier.urihttp://nur.nu.edu.kz/handle/123456789/2759
dc.language.isoenru_RU
dc.publisherInternational Journal of Infectious Diseasesru_RU
dc.rightsOpen Access - the content is available to the general publicru_RU
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectHPVru_RU
dc.subjectCINru_RU
dc.subjectcervicalru_RU
dc.subjectcancerru_RU
dc.subjectprevalenceru_RU
dc.subjectKazakhstanru_RU
dc.titleApplication of molecular genotyping to determine prevalence of HPV strains in Pap smears of Kazakhstan womenru_RU
dc.typeArticleru_RU

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