ACTIVITY-BASED PROBES TO UTILIZE THE PROTEOLYTIC ACTIVITY OF CATHEPSIN G IN BIOLOGICAL SAMPLES

dc.contributor.authorBurster, Timo
dc.contributor.authorGärtner, Fabian
dc.contributor.authorKnippschild, Uwe
dc.contributor.authorZhanapiya, Anuar
dc.date.accessioned2022-11-23T11:16:25Z
dc.date.available2022-11-23T11:16:25Z
dc.date.issued2021
dc.description.abstractNeutrophils, migrating to the site of infection, are able to release serine proteases after being activated. These serine proteases comprise cathepsin G (CatG), neutrophil elastase protease 3 (PR3), and neutrophil serine protease 4 (NSP4). A disadvantage of the uncontrolled proteolytic activity of proteases is the outcome of various human diseases, including cardiovascular diseases, thrombosis, and autoimmune diseases. Activity-based probes (ABPs) are used to determine the proteolytic activity of proteases, containing a set of three essential elements: Warhead, recognition sequence, and the reporter tag for detection of the covalent enzyme activity-based probe complex. Here, we summarize the latest findings of ABP-mediated detection of proteases in both locations intracellularly and on the cell surface of cells, thereby focusing on CatG. Particularly, application of ABPs in regular flow cytometry, imaging flow cytometry, and mass cytometry by time-of-flight (CyTOF) approaches is advantageous when distinguishing between immune cell subsets. ABPs can be included in a vast panel of markers to detect proteolytic activity and determine whether proteases are properly regulated during medication. The use of ABPs as a detection tool opens the possibility to interfere with uncontrolled proteolytic activity of proteases by employing protease inhibitors.en_US
dc.identifier.citationBurster, T., Gärtner, F., Knippschild, U., & Zhanapiya, A. (2021). Activity-Based Probes to Utilize the Proteolytic Activity of Cathepsin G in Biological Samples. Frontiers in Chemistry, 9. https://doi.org/10.3389/fchem.2021.628295en_US
dc.identifier.urihttp://nur.nu.edu.kz/handle/123456789/6825
dc.language.isoenen_US
dc.publisherFrontiers in Chemistryen_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectType of access: Open Accessen_US
dc.subjectCyTOF/mass cytometryen_US
dc.subjectactivity-based probesen_US
dc.subjectcathepsin Gen_US
dc.subjectflow cytometryen_US
dc.subjectimmune cellsen_US
dc.subjectserine proteasesen_US
dc.titleACTIVITY-BASED PROBES TO UTILIZE THE PROTEOLYTIC ACTIVITY OF CATHEPSIN G IN BIOLOGICAL SAMPLESen_US
dc.typeArticleen_US
workflow.import.sourcescience

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