THE EFFECTS OF PRECONDITIONED MESENCHYMAL STEM CELLS ON GASTRIC ULCER REGENERATION IN MICE
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Date
2020
Authors
Zhunusova, M.S.
Journal Title
Journal ISSN
Volume Title
Publisher
International conference "MODERN PERSPECTIVES FOR BIOMEDICAL SCIENCES: FROM BENCH TO BEDSIDE”; National Laboratory Astana
Abstract
Introduction: During the last decade, preconditioned mesenchymal stem cells (MSCs) have gained
much attention in the field of cell therapy due to their capacity to differentiate into different cell types
and to promote immunosuppressive effects in multiple diseases. However, there is little information,
however, about the therapeutic effects of preconditioned MSCs in gastrointestinal disorders. In the present
study, we examined whether transplantation of preconditioned MSCs could improve gastric ulcer
healing.
Methods: MSCs were isolated from compact bone of C57BL/6 mice. MSCs were cultured in α-MEM
containing 10% FBS and 1% antibiotics. Preconditioning of MSCs was performed with IFN-γ and TNF-α
for 24 h. Mice were divided into 3 groups: group 1(PBS), group 2(MSCs) and group 3 (preconditioned
MSCs). Induction of gastric ulcer was made with alcohol-acetic acid solution. Each stomach was isolated
for macroscopical and histological analysis. The levels of vascular endothelial growth factor (VEGF) and
prostaglandin E2(PGE2) in stomach ulcer tissue were measured with ELISA-kits.
Results: Our results showed that transplantation of preconditioned MSCs markedly improved the histopathology
of the gastric tissue in comparison to MSCs and PBS-treated groups. Also the result of ulcer
index was significantly decreased in preconditioned MSC-treated group at day 3 and 5 of the study comparing
with the PBS-treated group. ELISA data showed that preconditioned MSCs restored the levels of
PGE2to the normal levels and significantly increased the VEGF levels.
Conclusion: Thus, our results showed that transplantation of preconditioned MSC significantly improved
gastric ulcer healing in mice, possibly through the inhibition of inflammation and induction of
angiogenesis in the gastric mucosa via the secretion of VEGF and PGE2.
Description
Keywords
mesenchymal stem cells, preconditioning, cytokines, gastric ulcer, regeneration