DETECTION OF RNA VIRUSES FROM INFLUENZA AND HIV TO EBOLA AND SARS-COV-2: A REVIEW
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Date
2020-12-07
Authors
Bukasov, Rostislav
Dossym, Dina
Filchakova, Olena
Journal Title
Journal ISSN
Volume Title
Publisher
Royal Society of Chemistry
Abstract
RNA-based viruses likely make up the highest pandemic threat among all known pathogens in about the last
100 years, since the Spanish Flu of 1918 with 50 M deaths up to COVID-19. Nowadays, an efficient and
affordable testing strategy for such viruses have become the paramount target for the fields of virology
and bioanalytical chemistry. The detection of the viruses (influenza, hepatitis, HIV, Zika, SARS, Ebola,
SARS-CoV-2, etc.) and human antibodies to these viruses is described and tabulated in terms of the
reported methods of detection, time to results, accuracy and specificity, if they are reported. The review
is focused, but not limited to publications in the last decade. Finally, the limits of detection for each
representative publication are tabulated by detection methods and discussed. These methods include
PCR, lateral flow immunoassays, LAMP-based methods, ELISA, electrochemical methods (e.g.,
amperometry, voltammetry), fluorescence spectroscopy, AFM, SPR and SERS spectroscopy, silver
staining and CRISPR-Cas based methods, bio-barcode detection, and resonance light scattering. The
review is likely to be interesting for various scientists, and particularly helpful with information for
establishing interdisciplinary research.
Description
Keywords
RNA viruses, Detection of RNA viruses, influenza, HIV, SARS-CoV-2, Ebola, genetic material, viruses, Research Subject Categories::NATURAL SCIENCES::Chemistry
Citation
Bukasov, R., Dossym, D., & Filchakova, O. (2021). Detection of RNA viruses from influenza and HIV to Ebola and SARS-CoV-2: a review. Analytical Methods, 13(1), 34–55. https://doi.org/10.1039/d0ay01886d