Discovery of Actinomycetes from Extreme Environments with Potential to Produce Novel Antibiotics

Simple item page
dc.contributor.authorTrenozhnikova, Lyudmila
dc.contributor.authorAzizan, Azliyati
dc.date.accessioned2020-03-30T04:16:13Z
dc.date.available2020-03-30T04:16:13Z
dc.date.issued2018
dc.descriptionhttp://cajgh.pitt.edu/ojs/index.php/cajgh/article/view/337en_US
dc.description.abstractIntroduction: Antimicrobial-resistant pathogens pose serious challenges to healthcare institutions and health of the public. Thus, there is an urgent need for the discovery of new and effective antimicrobial agents. Microorganisms that exist in extreme environments such as those with high salinity or alkalinity, are known as extremophiles, and include various species of actinomycetes. The goal of this study is to discover novel antibiotics from extremophiles found in Kazakhstan that are effective against drug resistant pathogens. Methods: Soil from extreme environments of Kazakhstan was collected, and pure cultures of actinomycetes were isolated and cultured in modified Bennett’s broth with either high concentrations of salt or high pH to mimic extreme environments. Extracts obtained from selected actinomycetes strains were used to test for antimicrobial activity against Staphylococcus aureus, Escherichia coli and Aspergillus niger. Results: A total of 5936 strains of extremophile actinomycetes were isolated; from these, 2019 strains were further isolated into pure cultures. Of these, 415 actinomycetes strains that demonstrated antagonistic antibacterial activities were selected. These actinomycetes were further classified into groups and subgroups based on their responses to different culture conditions. Antimicrobial antagonism activity for some of the actinomycetes strains was dependent on culture conditions and development of aerial mycelia under extreme conditions. Conclusion: We identified several interesting candidate extracts with putative antimicrobial activities against several strains of drug resistant pathogens. Our research of the actinomycetes’ ability to produce antibiotics in the near-natural conditions provides a great opportunity to assess their biodiversity and distribution in the Central Asian region and to develop new methodological approaches to the screening of new antimicrobial agents.en_US
dc.identifier.citationTrenozhnikova, L., & Azizan, A. (2018). Discovery of Actinomycetes from Extreme Environments with Potential to Produce Novel Antibiotics. Central Asian Journal of Global Health, 7(1). https://doi.org/10.5195/cajgh.2018.337en_US
dc.identifier.issn2166-7403
dc.identifier.other10.5195/cajgh.2018.337
dc.identifier.urihttp://cajgh.pitt.edu/ojs/index.php/cajgh/article/view/337/262
dc.identifier.urihttp://nur.nu.edu.kz/handle/123456789/4556
dc.language.isoenen_US
dc.publisherUniversity of Pittsburgh, University Library Systemen_US
dc.relation.ispartofseriesCentral Asian Journal of Global Health;Vol 7, No 1 (2018)
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectactinomycetesen_US
dc.subjectantibioticsen_US
dc.subjectpathogensen_US
dc.subjectantibiotic resistanceen_US
dc.subjectpathogensen_US
dc.subjectnatural productsen_US
dc.subjectextremophilesen_US
dc.titleDiscovery of Actinomycetes from Extreme Environments with Potential to Produce Novel Antibioticsen_US
dc.typeArticleen_US
workflow.import.sourcescience

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Discovery of Actinomycetes from Extreme Environments with Potential to Produce Novel Antibiotics.pdf
Size:
1.31 MB
Format:
Adobe Portable Document Format
Description:
Article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
6 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Articles