Proposal for assessment of the antimicrobial efficacy of undiluted medical honey: Using a standardized phase 2/step 2 in vitro stainless steel disc carrier test model

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dc.contributor.authorGoerdt, Anna-Maria
dc.contributor.authorAssadian, Ojan
dc.contributor.authorRazavi, Behzad
dc.contributor.authorIgelbrink-Holter, Dorothee
dc.contributor.authorSimon, Arne
dc.contributor.authorHübner, Nils-Olaf
dc.contributor.authorPartecke, Lars Ivo
dc.contributor.authorZhumadilova, Anara
dc.contributor.authorHeidecke, Claus-Dieter
dc.contributor.authorKramer, Axel
dc.creatorAnna-Maria, Goerdt
dc.date.accessioned2017-12-22T03:34:44Z
dc.date.available2017-12-22T03:34:44Z
dc.date.issued2013-07-01
dc.description.abstractAbstract PurposeThe antimicrobial efficacy of medical honeys used for professional wound care was published previously. So far, all in vitro antimicrobial tests performed have been conducted using non-standardized methods and used diluted medical honey. A standardized, reproducible phase 2/step 2 in vitro disc carrier model is proposed allowing testing the antimicrobial efficacy of undiluted medical honey. MethodsUsing a standardized disc carrier model, the log10 Reduction Factor (RF) of undiluted medical honey was determined for MSSA, MRSA, VRE, Pseudomonas aeruginosa, and Candida albicans, with and without protein challenge. ResultsAn antimicrobial efficacy was observed after 1h. Without organic challenge, log10RF >5 within 24h was achieved for all tested microorganisms, except for Staphylococcus aureus (log10RF: 4.8/24h). Challenged with 10% bovine serum albumin, a similar pattern was observed at 24h. The most susceptible organism was C. albicans (log10RF of >3/5min). Over an exposure time of 48h, the tested medical honey's antimicrobial activity under protein challenge achieved log10RFs >6 for C. albicans and >7log10 for all tested bacteria. ConclusionsIt was demonstrated that the proposed standardized phase 2/step 2 in vitro stainless steel disc carrier test model is applicable for testing the antimicrobial activity of highly viscous and undiluted compounds such as medical honey. Undiluted medical honey exhibits a strong antimicrobial efficacy even under protein challenge after 1h, which increases to >6log10 for C. albicans, and >7log10 for Gr+ and Gr− bacteria.en_US
dc.identifierDOI:10.1016/j.wndm.2013.03.003
dc.identifier.citationAnna-Maria Goerdt, Ojan Assadian, Behzad Razavi, Dorothee Igelbrink-Holter, Arne Simon, Nils-Olaf Hübner, Lars Ivo Partecke, Anara Zhumadilova, Claus-Dieter Heidecke, Axel Kramer, Proposal for assessment of the antimicrobial efficacy of undiluted medical honey: Using a standardized phase 2/step 2 in vitro stainless steel disc carrier test model, In Wound Medicine, Volume 1, 2013, Pages 20-24en_US
dc.identifier.issn22139095
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S2213909513000049
dc.identifier.urihttp://nur.nu.edu.kz/handle/123456789/3018
dc.language.isoenen_US
dc.publisherWound Medicineen_US
dc.relation.ispartofWound Medicine
dc.rights.licenseCopyright © 2013 Elsevier GmbH. All rights reserved.
dc.subjectMedical honeyen_US
dc.subjectMedihoneyen_US
dc.subjectAntimicrobialen_US
dc.subjectMicrobicidalen_US
dc.subjectIn vitroen_US
dc.subjectMRSAen_US
dc.subjectCandida albicansen_US
dc.titleProposal for assessment of the antimicrobial efficacy of undiluted medical honey: Using a standardized phase 2/step 2 in vitro stainless steel disc carrier test modelen_US
dc.typeArticleen_US
elsevier.aggregationtypeJournal
elsevier.coverdate2013-07-01
elsevier.coverdisplaydateJuly 2013
elsevier.endingpage24
elsevier.identifier.doi10.1016/j.wndm.2013.03.003
elsevier.identifier.eid1-s2.0-S2213909513000049
elsevier.identifier.piiS2213-9095(13)00004-9
elsevier.identifier.scopusid84883758197
elsevier.openaccess0
elsevier.openaccessarticlefalse
elsevier.openarchivearticlefalse
elsevier.startingpage20
elsevier.teaserThe antimicrobial efficacy of medical honeys used for professional wound care was published previously. So far, all in vitro antimicrobial tests performed have been conducted using non-standardized...
elsevier.volume1
workflow.import.sourcescience

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