A CARBON DOT-BASED CO-NANOZYME WITH ALKALINE PHOSPHATASE – MECHANISM AND APPLICATION

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dc.contributor.authorFan, Haiyan
dc.contributor.authorDukenbayev, Kanat
dc.contributor.authorSun, Qinglei
dc.contributor.authorKhamijan, Medina
dc.contributor.authorTurdaliyev, Akhrorbek
dc.contributor.authorYsmaiyl, Alibek
dc.contributor.authorTassanbiyeva, Aigerim
dc.contributor.authorMa, Cuiping
dc.contributor.authorXie, Yingqiu
dc.date.accessioned2022-01-13T08:35:25Z
dc.date.available2022-01-13T08:35:25Z
dc.date.issued2021-10-21
dc.description.abstractElevated levels of alkaline phosphatase (ALP) are associated with bone metastasis, liver cancer, prostate cancer, breast cancer, and many other diseases or stem cell marker. It is therefore of great significance to quantitatively detect the ALP levels by a rapid, highly sensitive, and easy-to-use strip paper test. In the present work, we discovered an enhancement of ALP activity upon the addition of cauliflower-derived carbon dots (CFCDs), which can be applied as a sensor for ALP. The mixed ALP and CFCDs exhibited a typical Michaelis Menten mechanism with increased Vmax and reduced Km compared to ALP alone. High-Resolution Atomic Force Microscopy (HR-AFM) reveals the dimensions of ALP, the CFCDs, and the phosphatase substrate para-nitrophenyl phosphate (pNPP), as well as the potential interactions among them. The role of the CFCDs was identified as the addition of reaction centers to ALP; in other words, a competitive activator. Besides the improved kinetics, the yield of dephosphorylated product was also increased by at least twice upon the addition of CFCDs. Taking advantage of this effect, a portable CFCD-based paper strip assay was developed to achieve sensitive detection of abnormally elevated ALP levels and visualization of cancer stem cells or proteins by phosphatase-conjugated antibodies. Our findings show great promise for disease diagnosis and bioassays related to ALP enhancement that may be used for protein or cell detection.en_US
dc.identifier.citationFan, H., Dukenbayev, K., Sun, Q., Khamijan, M., Turdaliyev, A., Ysmaiyl, A., Tassanbiyeva, A., Ma, C., & Xie, Y. (2021). A carbon dot-based Co-nanozyme with alkaline phosphatase – mechanism and application. RSC Advances, 11(53), 33253–33259. https://doi.org/10.1039/d1ra04483den_US
dc.identifier.urihttp://nur.nu.edu.kz/handle/123456789/5975
dc.language.isoenen_US
dc.publisherRSC Advancesen_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectType of access: Open Accessen_US
dc.subjectalkaline phosphataseen_US
dc.subjectcanceren_US
dc.titleA CARBON DOT-BASED CO-NANOZYME WITH ALKALINE PHOSPHATASE – MECHANISM AND APPLICATIONen_US
dc.typeArticleen_US
workflow.import.sourcescience

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