Therapeutic potential of a scorpion venom-derived antimicrobial peptide and its homologs against antibiotic-resistant Gram-positive bacteria
| dc.contributor.author | Xie, Yingqiu | |
| dc.contributor.author | Liu, Gaomin | |
| dc.contributor.author | Yang, Fan | |
| dc.contributor.author | Li, Fangfang | |
| dc.contributor.author | Li, Zhongjie | |
| dc.contributor.author | Lang, Yange | |
| dc.contributor.author | Shen, Bingzheng | |
| dc.contributor.author | Wu, Yingliang | |
| dc.contributor.author | Li, Wenxin | |
| dc.contributor.author | Harrison, Patrick L. | |
| dc.contributor.author | Strong, Peter N. | |
| dc.contributor.author | Miller, Keith | |
| dc.contributor.author | Cao, Zhijian | |
| dc.date.accessioned | 2019-11-04T09:48:21Z | |
| dc.date.available | 2019-11-04T09:48:21Z | |
| dc.date.issued | 2018-05-28 | |
| dc.description.abstract | The alarming rise in the prevalence of antibiotic resistance among pathogenic bacteria poses a unique challenge for the development of effective therapeutic agents. Antimicrobial peptides (AMPs) have attracted a great deal of attention as a possible solution to the increasing problem of antibiotic-resistant bacteria. Marcin-18 was identified from the scorpion Mesobuthus martensii at both DNA and protein levels. The genomic sequence revealed that the marcin-18 coding gene contains a phase-I intron with a GT-AG splice junction located in the DNA region encoding the N-terminal part of signal peptide. The peptide marcin-18 was also isolated from scorpion venom. A protein sequence homology search revealed that marcin-18 shares extremely high sequence identity to the AMPs meucin-18 and megicin-18. In vitro, chemically synthetic marcin-18 and its homologs (meucin-18 and megicin-18) showed highly potent inhibitory activity against Gram-positive bacteria, including some clinical antibiotic-resistant strains. Importantly, in a mouse acute peritonitis model, these peptides significantly decreased the bacterial load in ascites and rescued nearly all mice heavily infected with clinical methicillin-resistant Staphylococcus aureus from lethal bacteremia. Peptides exerted antimicrobial activity via a bactericidal mechanism and killed bacteria through membrane disruption. Taken together, marcin-18 and its homologs have potential for development as therapeutic agents for treating antibiotic-resistant, Gram-positive bacterial infections. | en_US |
| dc.identifier.citation | Liu, G., Yang, F., Li, F., Li, Z., Lang, Y., Shen, B., ... Cao, Z. (2018). Therapeutic potential of a scorpion venom-derived antimicrobial peptide and its homologs against antibiotic-resistant Gram-positive bacteria. Frontiers in Microbiology, 9(MAY), [1159]. https://doi.org/10.3389/fmicb.2018.01159 | en_US |
| dc.identifier.uri | https://doi.org/10.3389/fmicb.2018.01159 | |
| dc.identifier.uri | http://nur.nu.edu.kz/handle/123456789/4288 | |
| dc.language.iso | en | en_US |
| dc.publisher | Nazarbayev University School of Sciences and Humanities | en_US |
| dc.rights | Attribution-NonCommercial-ShareAlike 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/3.0/us/ | * |
| dc.title | Therapeutic potential of a scorpion venom-derived antimicrobial peptide and its homologs against antibiotic-resistant Gram-positive bacteria | en_US |
| dc.type | Article | en_US |
| workflow.import.source | science |