DETERMINANTS OF RESISTANCE IN BACTEROIDES FRAGILIS STRAIN BFR_KZ01 ISOLATED FROM A PATIENT WITH PERITONITIS IN KAZAKHSTAN
| dc.contributor.author | Kozhakhmetova, Saniya | |
| dc.contributor.author | Zholdybayeva, Elena V. | |
| dc.contributor.author | Tarlykov, Pavel | |
| dc.contributor.author | Atavliyeva, Sabina | |
| dc.contributor.author | Syzdykov, Tleuli | |
| dc.contributor.author | Daniyarov, Asset | |
| dc.contributor.author | Mukhtarova, Kymbat | |
| dc.contributor.author | Ramankulov, Yerlan | |
| dc.date.accessioned | 2021-08-20T04:05:52Z | |
| dc.date.available | 2021-08-20T04:05:52Z | |
| dc.date.issued | 2021-03-02 | |
| dc.description.abstract | Objectives: Bacteroides fragilis is one of the most important human anaerobic pathogens often found in various clinical infections. The purpose of this study was to determine the susceptibility of a B. fragilis clinical strain (BFR_KZ01) from Kazakhstan to the most commonly used anti-anaerobic drugs at the local level and to detect genes associated with resistance to these antibiotics. Methods: Species identification of the bacterial isolate was performed by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS) and 16S rRNA gene sequencing. Susceptibility to broad-spectrum antibiotics (metronidazole, meropenem, ciprofloxacin, clindamycin and tetracycline) most commonly used for the treatment of intra-abdominal infections (IAIs) was determined. Mass spectra groups essential for identifying cfiA-positive strains among clinical isolates were studied using ClinProTools 3.0.22 software. An Ion Torrent PGM™ platform was used for whole-genome sequencing (WGS) of the studied isolate. Results: The resulting WGS data of strain BFR_KZ01 was submitted to GenBank. In total, 5300 coding sequences (CDSs) and 69 RNA genes were determined. Analysis of the whole-genome data revealed that the studied strain harbours cfiA, nimB, tetQ and gyrA genes conferring resistance to key drugs used in treatment of the IAIs. MALDI-TOF/MS analysis assigned strain BFR_KZ01 to Group II (cfiA-positive); however, BFR_KZ01 was phenotypically sensitive to meropenem (mean MIC, 1.3 mg/L). Conclusion: Determinants of drug resistance in strain BFR_KZ01 were identified. It was revealed that B. fragilis strain BFR_KZ01 from Kazakhstan is multidrug-resistant since it carries nimB, tetQ and gyrA genes conferring resistance to metronidazole, tetracycline and ciprofloxacin. | en_US |
| dc.identifier.citation | Kozhakhmetova, S., Zholdybayeva, E., Tarlykov, P., Atavliyeva, S., Syzdykov, T., Daniyarov, A., Mukhtarova, K., & Ramankulov, Y. (2021). Determinants of resistance in Bacteroides fragilis strain BFR_KZ01 isolated from a patient with peritonitis in Kazakhstan. Journal of Global Antimicrobial Resistance, 25, 1–4. https://doi.org/10.1016/j.jgar.2021.02.022 | en_US |
| dc.identifier.issn | 2213-7165 | |
| dc.identifier.uri | https://doi.org/10.1016/j.jgar.2021.02.022 | |
| dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S2213716521000540?via%3Dihub | |
| dc.identifier.uri | http://nur.nu.edu.kz/handle/123456789/5696 | |
| dc.language.iso | en | en_US |
| dc.publisher | Elsevier Ltd | en_US |
| dc.relation.ispartofseries | Journal of Global Antimicrobial Resistance;Volume 25, June 2021, Pages 1-4 | |
| dc.rights | Attribution-NonCommercial-ShareAlike 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/3.0/us/ | * |
| dc.subject | Type of access: Open Access | en_US |
| dc.subject | Bacteroides fragilis | en_US |
| dc.subject | Intra-abdominal infection | en_US |
| dc.subject | Peritonitis | en_US |
| dc.subject | Resistance gene | en_US |
| dc.subject | Whole-genome sequencing | en_US |
| dc.title | DETERMINANTS OF RESISTANCE IN BACTEROIDES FRAGILIS STRAIN BFR_KZ01 ISOLATED FROM A PATIENT WITH PERITONITIS IN KAZAKHSTAN | en_US |
| dc.type | Article | en_US |
| workflow.import.source | science |