Application of molecular genotyping to determine prevalence of HPV strains in Pap smears of Kazakhstan women
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Date
2017-01-01
Authors
Niyazmetova, Luiza
Aimagambetova, Gulzhanat
Stambekova, Nazigul
Abugalieva, Zaurech
Seksembayeva, Korlukain
Ali, Syed
Azizan, Azliyati
Journal Title
Journal ISSN
Volume Title
Publisher
International Journal of Infectious Diseases
Abstract
Abstract ObjectivesHuman papillomavirus is the main causative agent for cervical cancer. However, few data are available about HPV prevalence in Kazakhstan. The aims of this study were to genotype HPV DNA in Pap smear samples of women to determine prevalence of carcinogenic HPV types in Astana, Kazakhstan and to analyze the association between HPV positivity and the cytology results of patient samples. MethodsPap smear materials were obtained from 140 patients aged 18-59, who visited the outpatient gynecological clinic. Microscopic examination was done to detect dysplasia, and HPV genotyping was done using real-time multiplex PCR. ResultsHPV testing showed that among 61 HPV positive patients, the most prevalent types were 16 and 18. Microscopic examination showed that 79% of the samples had normal cytology, while 13% had CIN grade I, 5% had CIN grade II, and 3% had CIN grade III. The analysis revealed that 12% of the samples had CIN cytology and presence of HPV. Approximately 31% had HPV without cervical dysplasia, while 8% of samples were CIN positive without HPV infection. A statistically significant relationship between HPV 16 and HPV 33 positive samples and CIN grade II and III was found. ConclusionsOverall, this study will help to strengthen and guide health policy implementation of primary and secondary cervical cancer prevention strategies in Kazakhstan.
Description
Keywords
HPV, CIN, cervical cancer, prevalence, Kazakhstan
Citation
Luiza Niyazmetova, Gulzhanat Aimagambetova, Nazigul Stambekova, Zaurech Abugalieva, Korlukain Seksembayeva, Syed Ali, Azliyati Azizan, Application of molecular genotyping to determine prevalence of HPV strains in Pap smears of Kazakhstan women, In International Journal of Infectious Diseases, Volume 54, 2017, Pages 85-88