IN VITRO COMPARATIVE ANALYSIS OF CYCLOHEXIMIDE TUMORICIDAL EFFECTS ON GLIOBLASTOMA AND NEUROGLIOMA CELL LINES

Abstract

Neuroglioma (NGM) from epithelial origin is a non-malignant, but yet dangerous solid tumor, whereas glioblastoma (GBM) from mixed origin is a malignant and most threatening type of brain tumor. Cycloheximide (CHX), which is generally used to inhibit cellular protein synthesis, has been reported to promote or lead to cell death in various tumor cell types, including brain tumor. However, the tumor suppressing effect of CHX differs in tumor cell types. In this Master’s Thesis two different cell lines NGM and GBM were used to analyze the effects of CHX on them. Through MTT cell viability assay the results have shown that the effects of CHX on NGM and GBM vary at a significant extent. The IC50 concentration of CHX for GBM (0.385 mM) was found to be ~4.62% higher than of NGM (0.368 mM), suggesting that GBM requires more concentrated cytotoxic agent solution to reach 50% of cell viability. The trends from abovementioned experiment have been conserved when wound scratch assay was performed at different concentrations of CHX. Overall, a compelling dissimilarity was concluded from the experiments for CHX effects on NGM and GBM.