Simian Virus 40 Large T Antigen as a Model to Test the Efficacy of Flouroquinolones against Viral Helicases

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dc.contributor.authorSiddiqui, Sammer
dc.contributor.authorAnwar, Muhammad F.
dc.contributor.authorNaeem, Sadaf
dc.contributor.authorAbidi, Syed Hani
dc.contributor.authorZarina, Shamshad
dc.contributor.authorAli, Syed
dc.date.accessioned2020-05-19T10:44:25Z
dc.date.available2020-05-19T10:44:25Z
dc.date.issued2018-02-28
dc.description.abstractSimian virus 40 large T-antigen (SV40 LT-Ag) is a 708 amino acid nuclear phosphoprotein. Among many functions of LT-Ag is its ability to perform as an ATPase-helicase, catalyzing the unwinding of viral genome during replication. The LT-Ag has been employed in the studies of helicase structure and function, and has served as a model helicase for the screening of antiviral drugs that target viral helicase. In this study, using in vitro enzyme assays and in silico computer modeling, we screened a batch of 18 fluoroquinolones to assess their potential as antivirals by virtue of their inhibition of the LT-Ag helicase. We found all fluoroquinolones to be inhibitory to the helicase activity of LT-Ag. In our docking analysis, most of these tested drugs showed similarity in their interactions with LT-Ag. Our study shows the potential of fluoroquinolones as antiviral drugs and of SV40 LT-Ag as a model protein for screening drugs against viral helicases.en_US
dc.identifier.citationSiddiqui, S., Anwar, M. F., Naeem, S., Abidi, S. H., Zarina, S., & Ali, S. (2018). Simian Virus 40 Large T Antigen as a Model to Test the Efficacy of Flouroquinolones against Viral Helicases. Bioinformation, 14(2), 75–79. https://doi.org/10.6026/97320630014075en_US
dc.identifier.issn0973-2063
dc.identifier.other10.6026/97320630014075
dc.identifier.urihttp://www.bioinformation.net/014/97320630014075.htm
dc.identifier.urihttps://doi.org/10.6026/97320630014075
dc.identifier.urihttp://nur.nu.edu.kz/handle/123456789/4735
dc.language.isoenen_US
dc.publisherBiomedical Informaticsen_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectSV40 LT-Agen_US
dc.subjectFluoroquinolonesen_US
dc.subjectAntiviral drugsen_US
dc.subjectViral Helicasesen_US
dc.subjectResearch Subject Categories::MEDICINEen_US
dc.titleSimian Virus 40 Large T Antigen as a Model to Test the Efficacy of Flouroquinolones against Viral Helicasesen_US
dc.typeArticleen_US
workflow.import.sourcescience

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