Simian Virus 40 Large T Antigen as a Model to Test the Efficacy of Flouroquinolones against Viral Helicases
dc.contributor.author | Siddiqui, Sammer | |
dc.contributor.author | Anwar, Muhammad F. | |
dc.contributor.author | Naeem, Sadaf | |
dc.contributor.author | Abidi, Syed Hani | |
dc.contributor.author | Zarina, Shamshad | |
dc.contributor.author | Ali, Syed | |
dc.date.accessioned | 2020-05-19T10:44:25Z | |
dc.date.available | 2020-05-19T10:44:25Z | |
dc.date.issued | 2018-02-28 | |
dc.description.abstract | Simian virus 40 large T-antigen (SV40 LT-Ag) is a 708 amino acid nuclear phosphoprotein. Among many functions of LT-Ag is its ability to perform as an ATPase-helicase, catalyzing the unwinding of viral genome during replication. The LT-Ag has been employed in the studies of helicase structure and function, and has served as a model helicase for the screening of antiviral drugs that target viral helicase. In this study, using in vitro enzyme assays and in silico computer modeling, we screened a batch of 18 fluoroquinolones to assess their potential as antivirals by virtue of their inhibition of the LT-Ag helicase. We found all fluoroquinolones to be inhibitory to the helicase activity of LT-Ag. In our docking analysis, most of these tested drugs showed similarity in their interactions with LT-Ag. Our study shows the potential of fluoroquinolones as antiviral drugs and of SV40 LT-Ag as a model protein for screening drugs against viral helicases. | en_US |
dc.identifier.citation | Siddiqui, S., Anwar, M. F., Naeem, S., Abidi, S. H., Zarina, S., & Ali, S. (2018). Simian Virus 40 Large T Antigen as a Model to Test the Efficacy of Flouroquinolones against Viral Helicases. Bioinformation, 14(2), 75–79. https://doi.org/10.6026/97320630014075 | en_US |
dc.identifier.issn | 0973-2063 | |
dc.identifier.other | 10.6026/97320630014075 | |
dc.identifier.uri | http://www.bioinformation.net/014/97320630014075.htm | |
dc.identifier.uri | https://doi.org/10.6026/97320630014075 | |
dc.identifier.uri | http://nur.nu.edu.kz/handle/123456789/4735 | |
dc.language.iso | en | en_US |
dc.publisher | Biomedical Informatics | en_US |
dc.rights | Attribution-NonCommercial-ShareAlike 3.0 United States | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/3.0/us/ | * |
dc.subject | SV40 LT-Ag | en_US |
dc.subject | Fluoroquinolones | en_US |
dc.subject | Antiviral drugs | en_US |
dc.subject | Viral Helicases | en_US |
dc.subject | Research Subject Categories::MEDICINE | en_US |
dc.title | Simian Virus 40 Large T Antigen as a Model to Test the Efficacy of Flouroquinolones against Viral Helicases | en_US |
dc.type | Article | en_US |
workflow.import.source | science |
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