FUNCTIONAL CHARACTERIZATION OF OSMOTIC STRESS RESPONSE IN HALOARCHAEA ISOLATED FROM THE KOBEITUZ LAKE

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dc.contributor.authorBissenbayev, Kudaybergen
dc.date.accessioned2024-06-05T09:10:49Z
dc.date.available2024-06-05T09:10:49Z
dc.date.issued2024-04-11
dc.description.abstractHalobacterium KBTZ01, a halophile archaea species from Kobeituz Lake, Kazakhstan, thrives in hypersaline environments. Therefore, these serve as good models for studying molecular mechanisms underlying the tolerance to high salt concentrations. This study investigates the impact of osmotic stress on these cells, induced by exposure to varying NaCl concentrations (0.6/1/1.5M) in a hypotonic growth medium at 3 and 9 hours. The experimental approach involves studying stress response by incubating cells in hypotonic media, followed by protein extraction and analysis using SDS-PAGE and mass spectrometry. The morphology and viability parameters were analyzed with confocal microscopy. Low NaCl concentrations (600) are deadly for the haloarchaea, while 1M and 1.5M NaCl can be tolerated for a certain amount of time. Proteomic findings include upregulation of certain proteins related to metabolism, biogenesis and signal processing. Significant decrease in motility was observed and was validated by downregulation of motility proteins. Some proteins indicate varying abundance levels with progression of hypotonic stress. This research contributes valuable insights into the molecular mechanisms enabling halophilic archaea to thrive in challenging environments.en_US
dc.identifier.citationBissenbayev, K. (2024). Functional Characterization Of Osmotic Stress Response In Haloarchaea Isolated From The Kobeituz Lake. Nazarbayev University School of Sciences and Humanitiesen_US
dc.identifier.urihttp://nur.nu.edu.kz/handle/123456789/7748
dc.language.isoen_USen_US
dc.publisherNazarbayev University School of Sciences and Humanitiesen_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.subjecthaloarchaea, proteomics, Kobeituz, archaeaen_US
dc.subjectType of access: Restricteden_US
dc.subjecthaloarchaeaen_US
dc.subjectproteomicsen_US
dc.subjectKobeituzen_US
dc.subjectarchaeaen_US
dc.titleFUNCTIONAL CHARACTERIZATION OF OSMOTIC STRESS RESPONSE IN HALOARCHAEA ISOLATED FROM THE KOBEITUZ LAKEen_US
dc.typeBachelor's thesisen_US
workflow.import.sourcescience

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