SCREENING FOR INHIBITORS OF DIFFERENT KRAS MUTANTS, A KEY ONCOGENE IN NONSMALL-CELL LUNG CANCER

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dc.contributor.authorTurmagambetova, Madina
dc.date.accessioned2024-10-07T09:21:17Z
dc.date.available2024-10-07T09:21:17Z
dc.date.issued2024
dc.description.abstractNonsmall-cell lung cancer is the most prevalent type of lung cancer accounting for 85% of all lung cancer cases. Ki-ras2 Kirsten rat sarcoma viral oncogene homolog(KRAS) belongs to the RAS family. KRAS is a common oncogenic signal transducer protein predominantly mutated in cancers at positions G12, G13, and Q61. In this study, we successfully knocked out the coding region(Exon 2) of KRAS in the H1299 cell line by CRISPR/Cas9 system. Overall 8 positive clones were detected from 128 individual colonies. The clonal subpopulations have shown various growth rates. Some clones showed higher growth rates, while others grew more slowly, due to the off-targeted effects of the CRISPR/Cas9 system. The deletion efficiency was proved by the Sanger sequencing analysis.
dc.identifier.citationTurmagambetova, M. (2024). Screening for inhibitors of different KRAS mutants, a key oncogene in nonsmall-cell lung Cancer. Nazarbayev University School of Medicine
dc.identifier.urihttps://nur.nu.edu.kz/handle/123456789/8269
dc.language.isoen
dc.publisherNazarbayev University School of Medicine
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United Statesen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/
dc.subjectKRAS mutants
dc.subjectType of access: Embargo
dc.titleSCREENING FOR INHIBITORS OF DIFFERENT KRAS MUTANTS, A KEY ONCOGENE IN NONSMALL-CELL LUNG CANCER
dc.typeMaster`s thesis

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