Amyloid-b peptide on sialyl-LewisX-selectin-mediated membrane tether mechanics at the cerebral endothelial cell surface

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dc.contributor.authorAskarova, Sholpan
dc.contributor.authorSun, Zhe
dc.contributor.authorSun, Grace Y.
dc.contributor.authorMeininger, Gerald A.
dc.contributor.authorLee, James C-M.
dc.date.accessioned2016-02-08T05:56:50Z
dc.date.available2016-02-08T05:56:50Z
dc.date.issued2013-04-12
dc.description.abstractIncreased deposition of amyloid-b peptide (Ab) at the cerebral endothelial cell (CEC) surface has been implicated in enhancement of transmigration of monocytes across the brain blood barrier (BBB) in Alzheimer’s disease (AD). In this study, quantitative immunofluorescence microscopy (QIM) and atomic force microscopy (AFM) with cantilevers biofunctionalized by sialyl-Lewisx (sLex) were employed to investigate Ab-altered mechanics of membrane tethers formed by bonding between sLex and p-selectin at the CEC surface, the initial mechanical step governing the transmigration of monocytes. QIM results indicated the ability for Ab to increase p-selectin expression at the cell surface and promote actin polymerization in both bEND3 cells (immortalized mouse CECs) and human primary CECs. AFM data also showed the ability for Ab to increase cell stiffness and adhesion probability in bEND3 cells. On the contrary, Ab lowered the overall force of membrane tether formation (Fmtf), and produced a bimodal population of Fmtf, suggesting subcellular mechanical alterations in membrane tethering. The lower Fmtf population was similar to the results obtained from cells treated with an F-actin-disrupting drug, latrunculin A. Indeed, AFM results also showed that both Ab and latrunculin A decreased membrane stiffness, suggesting a lower membrane-cytoskeleton adhesion, a factor resulting in lower Fmtf. In addition, these cerebral endothelial alterations induced by Ab were abrogated by lovastatin, consistent with its anti-inflammatory effects. In sum, these results demonstrated the ability for Ab to enhance p-selectin expression at the CEC surface and induce cytoskeleton reorganization, which in turn, resulted in changes in membrane-cytoskeleton adhesion and membrane tethering, mechanical factors important in transmigration of monocytes through the BBB.ru_RU
dc.description.sponsorshipThis work was supported by Alzheimer Association Grant NIRG-06-24448; NIH Grant 1P01 AG18357, R21NS052385, 5R21AG032579 and in part by 1P01HL095486 and AHA 0835676N; ‘‘Bolashak’’ scholarship and Ministry of Education and Science of the Republic of Kazakhstan 1029/GF2. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.ru_RU
dc.identifier.citationAskarova S, Sun Z, Sun GY, Meininger GA, Lee JC-M (2013) Amyloid-b Peptide on Sialyl-LewisX-Selectin-Mediated Membrane Tether Mechanics at the Cerebral Endothelial Cell Surface. PLoS ONE 8(4): e60972. doi:10.1371/journal.pone.0060972ru_RU
dc.identifier.urihttp://nur.nu.edu.kz/handle/123456789/1172
dc.language.isoenru_RU
dc.publisherPLOS ONE:Open Access journalru_RU
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.subjectAmyloid-bru_RU
dc.subjectsialyl-lewisX-selectin-mediated membraneru_RU
dc.subjectcerebral endothelial cell surfaceru_RU
dc.subjectAlzheimer’s diseaseru_RU
dc.subjectneurodegenerative diseaseru_RU
dc.titleAmyloid-b peptide on sialyl-LewisX-selectin-mediated membrane tether mechanics at the cerebral endothelial cell surfaceru_RU
dc.typeArticleru_RU

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