BIOCHEMICAL AND ELECTROCHEMICAL CHARACTERIZATION OF BIOFILMS FORMED ON EVEROLIMUS-ELUTING CORONARY STENTS
Loading...
Date
2022
Authors
Akhmetzhan, Gauhar
Olaifa, Kayode
Kitching, Michael
Cahill, Paul A.
Pham, Tri T.
Ajunwa, Obinna M.
Marsili, Enrico
Journal Title
Journal ISSN
Volume Title
Publisher
Enzyme and Microbial Technology
Abstract
Drug-eluting stents (DES) are mostly used in percutaneous coronary intervention, which is the main treatment for
coronary artery occlusion. This procedure aims to restore the natural lumen, while minimizing the risk of
restenosis. However, stent insertion increases the risk for infections, due to contamination of the device or
insertion hub with normal skin flora. While coronary stent infection is a rare complication, it can be fatal.
Currently, there is little information on biofilm formation on everolimus-eluting stents. Although everolimus is
not designed as an antimicrobial agent, its antimicrobial activity should be investigated. In this study, biofilm
formation on everolimus-eluting and bare metal stents (BMS) is characterized through biochemical and electrochemical
methods. DES and BMS are inoculated with Pseudomonas aeruginosa and Staphylococcus epidermidis,
both independently and in co-culture. Biofilms formed on DES were 49.6 %, 12.9 % and 47.5 % higher than on
BMS for P. aeruginosa, S. epidermidis and their co-culture, respectively. Further, the charge output for DES was
18.9 % and 59.7 % higher than BMS for P. aeruginosa and its co-culture with S. epidermidis, respectively. This
observation is most likely due to higher surface roughness of DES, which favors biofilm formation. This work
shows that bioelectrochemical methods can be used for rapid detection of biofilms on drug-eluting and bare
metal stents, which may find application in quality assessment of stents and in characterization of stents removed
after polymicrobial infections.
Description
Keywords
Type of access: Open Access, Drug-eluting stent, Staphylococcus epidermidis, Pseudomonas aeruginosa, Biofilm, Bioelectrochemistry
Citation
Akhmetzhan, G., Olaifa, K., Kitching, M., Cahill, P. A., Pham, T. T., Ajunwa, O. M., & Marsili, E. (2023). Biochemical and electrochemical characterization of biofilms formed on everolimus-eluting coronary stents. Enzyme and Microbial Technology, 163, 110156. https://doi.org/10.1016/j.enzmictec.2022.110156