FUNCTIONAL ANALYSIS OF TYRO3 ISOFORMS IN BLADDER CANCER CELLS

Abstract

Bladder cancer (BC) is the 10th most common cancer type both in men and women and its incidence increases with age. TYRO3 is increased in 50% of muscle invasive bladder cancer (MIBCs), and TYRO3 overexpression confers TYRO3 dependence on bladder tumor cells. The downregulation of TYRO3 was linked to a decrease in bladder cancer cell growth and TYRO3 knockdown caused bladder cancer cell cycle arrest. The utilization of distinct BC cell lines in the investigations may imply that inhibiting TYRO3 has distinct effects. This thesis work hypothesizes that alternatively spliced TYRO3 isoform 2 is present in the nucleus of T24 and RT112 bladder cancer cells and regulates gene expression. Molecular cloning techniques were used to construct the recombinant plasmid, which were further transfected into T24 and RT112 cell lines and expression of protein were analyzed by Western Blotting method. The results of the thesis project are thoroughly presented and future perspectives are described.