GUT MICROBIOME ALTERATIONS IN SENIORS SUFFERING FROM ALZHEIMER’S DISEASE
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Date
2020
Authors
Askarova, Sholpan
Kaiyrlykyzy, Aiym
Kozhakhmetov, Samat S.
Nurgaziyev, Madiyar
Kushugulova, Almagul R.
Journal Title
Journal ISSN
Volume Title
Publisher
International conference "MODERN PERSPECTIVES FOR BIOMEDICAL SCIENCES: FROM BENCH TO BEDSIDE”; National Laboratory Astana
Abstract
Introduction: One of the important factors influencing human health and attracting increasing attention
of scientists during the last two decades is gut microbiome. It has been demonstrated that the
links exist between gut microbiome density and composition and a number of pathological conditions
including diabetes, obesity and cardiovascular diseases. These diseases, in turn, are the established risk
factors for the development of Alzheimer’s disease (AD). Moreover, there is data indicating that gut microbiome
can directly affect brain functions. However, only few studies have characterized the human
gut microbiome communities associated with AD. Therefore, more research is needed in order to reveal
the relationships existing between gut microbiome and brain functions and their influence on the development
and progression of AD.
Material and methods: Stool samples were obtained from patients with AD (n =11) and cognitively
normal age- and sex-matched participants (n =13). The composition of gut microbiome was characterized
by 16S ribosomal RNA MiSeq sequencing. Data analysis was performed using an independent computational
pipeline, less OTUs scripts (LotuS) [Hildebrand, F., 2013], SILVA reference database were used
as reference for 16S rRNA alignment. Statistical analyses were performed using R version 3.0.2.
Results: Our preliminary results demonstrated that gut microbiota of AD individuals had overall higher
α-diversity compared to healthy controls, although this difference was not significant, while β-diversity
analysis has revealed statistical significance (R-squared: 0.075975; p-value <0.033). Among bacterial
genera, microbiome of AD participants was characterized by a preponderance of Eubacterium copros,
Lachnospiraceae NK, Rikenellaceae RC9, Christensenellacea, Prevotella, Ruminococcus torgue, Parabacterides,
Coprococcus and Corynebacterium (LDA score [log10] > 3), whereas the healthy microbiome was characterized
by a preponderance of Lactobacillus, Holdemania, Holdemanella, Granucatella (LDA score [log10] >
3). The relative abundances of Lachnospiraceae, Lactobacillus, Eubacterium, and Odoribacter were significantly
different in AD patients compared to healthy participants (p < 0.01). Our data are consistent with
the results of Vogt et al. (2017) showing that in patients with AD the dominant families were Lachnospiraceae
and Ruminococcaceae.
Conclusion: Distinct microbial communities were associated with patients with AD when compared
with cognitively healthy seniors. However, more data is needed to ascertain our findings.
Description
Keywords
gut microbiome, Alzheimer’s disease, Research Subject Categories::MEDICINE