NEW TREATMENT STRATEGY AGAINST ISCHEMIC BRAIN DAMAGE BASED ON COMBINED APPLICATION OF STEM CELL THERAPY AND PLANT POLYPHENOLS
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Date
2020
Authors
Nurkenov, T.T.
Tsoy, A.K.
Zhusupova, G.E.
Olzhayev, F.S.
Ogay, V.B.
Umbayev, B.A.
Shalakhmetova, T.M.
Askarova, Sh.N.
Journal Title
Journal ISSN
Volume Title
Publisher
International conference "MODERN PERSPECTIVES FOR BIOMEDICAL SCIENCES: FROM BENCH TO BEDSIDEā; National Laboratory Astana
Abstract
Introduction: There is a number of data indicating that plant polyphenols can provide protection
against neurodegenerative changes associated with cerebral ischemia. On the other hand, it has been
shown that application of bone marrow-derived mesenchymal stem cells (BM-MSCs) resulted in an improved
functional recovery in animal models of cerebral ischemia and stroke by modulating the inflammatory
response, and stimulating endogenous neurogenesis and angiogenesis. Thus, we hypothesized
that neuroregenerative potential of mesenchymal stem cell therapy could be significantly improved if
applied in combination with plant polyphenols. We applied extract of Limonium Gmelinii (a plant widespread
in Kazakhstan) as a source of plant polyphenols.
Methods: 25 female Wistar rats were used in this study (weight 280-300g). Ischemic stroke was induced
in 20 animals by occlusion of the middle cerebral artery (MCAO); 15 animals received either Limonium
Gmelini, or BM-MSCs, and both; untreated and intact animals served as controls. The day before the
induction of stroke, the next day, on the 14th and 28th days after MCAO the sensorimotor functions of
the animals were evaluated. Before transplantation, BM-MSCs were transfected with lentiviral particles.
Results: The distribution of the transfected MSCs inside the body of laboratory animal with MCAO
was shown to be different from the distribution of MSCs inside the body of intact rats (control group).
In control rats all the MSCs were localized in visceral organs, while in rats with surgically induced MCAO
some amount of cells was found in the brains. Daily treatment of rat with 200 mg/kg of Limonium Gmelinii
extract for 28 days or single transplantation of MSC (5x10e6 cells) showed partial restoration of the
locomotor function of the animals while combined therapy with Limonium Gmelinii and MSCs completely
restores it by 28th day.
Conclusion: Combined therapy with extract of Limonium Gmelinii and mesenchymal stem cells is a
more effective approach in comparison to monotherapy.
Description
Keywords
mesenchymal stem cells, stroke, Limonium Gmelinii, polyphenols, rat, Research Subject Categories::MEDICINE