Protocol optimization for abtaining lyophilized human amniotic membrane extract
Loading...
Date
2016-05
Authors
Shramko, A.
Umbayev, В.
Olzhayev, R.
Idrissova, D.
Masoud, A.
Sadyrbekov, D.
Askarova, Sh.
Journal Title
Journal ISSN
Volume Title
Publisher
National Laboratory Astana, NAZARBAYEV UNIVERSITY
Abstract
The amniotic membrane (AM) is the inner amniotic fluid-filled membrane which
forms a cavity around the embryo. AM has long been used in the treatment of venous ulcers
and burns. Freshly extracted AM has been shown to possess the highest effect, thus making
the extraction method costly and severely limiting its possible usage. To counter this problem,
several extraction methods have been developed aimed at preserving the growth factors
present in AM and maintaining the AM in a sterile condition. The use of lyophilized AM extract
could help preserve its active properties which is associated with the presence of a variety of
cytokines necessary for wound-healing. However, there are no standard methods for obtaining
amniotic-membrane extracts to date. We chose two available methods in which the resulting
extract had the highest regenerative properties. Comparative analyses of these two methods
were not carried out. Hence, in order to determine which method gave AM lyophilisates with the
highest cytokine composition, we determined the cytokine content of the obtained lyophilisates
with reference to that of the native membrane.
Description
Keywords
amniotic membrane
Citation
Shramko A., Umbayev В., Olzhayev R, Idrissova D., Masoud A., Sadyrbekov D, and Askarova Sh. 2016. Protocol optimization for abtaining lyophilized human amniotic membrane extract. Abstract book. 4 th International Scientific Conference “Regenerative medicine & healthy aging”. National Laboratory Astana, Nazarbayev University. http://nur.nu.edu.kz/handle/123456789/1546