dc.contributor.author |
Fanning, Alan S.
|
|
dc.contributor.author |
Little, Brent P.
|
|
dc.contributor.author |
Rahner, Christoph
|
|
dc.contributor.author |
Utepbergenov, Darkhan
|
|
dc.contributor.author |
Walther, Zenta
|
|
dc.contributor.author |
Anderson, James M.
|
|
dc.date.accessioned |
2016-01-22T08:16:28Z |
|
dc.date.available |
2016-01-22T08:16:28Z |
|
dc.date.issued |
2006 |
|
dc.identifier.citation |
Alan S. Fanning, Brent P. Little, Christoph Rahner, Darkhan Utepbergenov, Zenta Walther, and James M. Anderson; 2006; The Unique-5 and -6 Motifs of ZO-1 Regulate Tight Junction Strand Localization and Scaffolding Properties; Molecular Biology of the Cell |
ru_RU |
dc.identifier.uri |
http://nur.nu.edu.kz/handle/123456789/1034 |
|
dc.description.abstract |
The proper cellular location and sealing of tight junctions is assumed to depend on scaffolding properties of ZO-1, a member of the MAGUK protein family. ZO-1 contains a conserved SH3-GUK module that is separated by a variable region (unique-5), which in other MAGUKs has proven regulatory functions. To identify motifs in ZO-1 critical for its
putative scaffolding functions, we focused on the SH3-GUK module including unique-5 (U5) and unique-6 (U6), a motif immediately C-terminal of the GUK domain. In vitro binding studies reveal U5 is sufficient for occludin binding; U6 reduces the affinity of this binding. In cultured cells, U5 is required for targeting ZO-1 to tight junctions and removal of U6 results in ectopically displaced junction strands containing the modified ZO-1, occludin, and claudin on the lateral cell membrane. These results provide evidence that ZO-1 can control the location of tight junction transmembrane proteins and reveals complex protein binding and targeting signals within its SH3-U5-GUK-U6 region. We review these findings in the context of regulated scaffolding functions of other MAGUK proteins |
ru_RU |
dc.language.iso |
en |
ru_RU |
dc.subject |
Research Subject Categories::NATURAL SCIENCES::Chemistry |
ru_RU |
dc.subject |
MAGUK protein |
ru_RU |
dc.title |
The Unique-5 and -6 Motifs of ZO-1 Regulate Tight Junction Strand Localization and Scaffolding Properties |
ru_RU |
dc.type |
Article |
ru_RU |