Browsing by Subject "In Vitro Techniques"

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Bacterial expression, purification and in Vitro phosphorylation of full-length ribosomal S6 kinase 2 (RSK2)

Utepbergenov, Darkhan; Hennig, Paulina M.; Derewenda, Urszula; Artamonov, Mykhaylo V.; Somlyo, Avril V.; Derewenda, Zygmunt S. (PLoS One, 2016-10-01)

Ribosomal S6 kinases (RSK) play important roles in cell signaling through the mitogen-activated protein kinase (MAPK) pathway. Each of the four RSK isoforms (RSK1-4) is a single polypeptide chain containing two kinase ...