THE EFFECT OF ARTEMISIA EXTRACTS ON HUMAN ASTROCYTOMA CELL VIABILITY IN VITRO
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Date
2022-05
Authors
Sailike, Bayan
Journal Title
Journal ISSN
Volume Title
Publisher
Nazarbayev University School of Sciences and Humanities
Abstract
Gliomas are one of the most aggressive and prevalent primary tumors of the central nervous
system originating from neuroglial cells. Although the current treatment methods such as
surgery, chemotherapy, and radiotherapy play a crucial role, the side effects and drug
resistance limit the efficiency and further affect patients’ health. The use of herbal medicine
for the prevention and treatment potential of tumor has drawing attention due to their fewer
toxic side effects than conventional treatments. The Genus Artemisia L. is one of the largest
genera in the Asteraceae family, widely distributed in Central Asia. Many of the Artemisia
species have been used in various treatments since ancient times as folk remedies. Some of
them have antitumor potential. However, it is unknown whether Artemisia terrae-albae
Krasch (AT), a common species endemic to Kazakhstan, has an antitumor effect on human
astrocytoma. In this study, we investigated the effect of AT on H4 astrocytoma cell line and
CTX-TNA2 astrocyte cell line (control) by determining its cytotoxic effect using alamarBlue
cell viability assay and quantifying cell apoptosis and reactive oxygen species (ROS) levels
using annexin V-FITC/PI staining and MitoSOX red staining, respectively. The results of cell
viability assay with alamarBlue™ reagent showed that the treatment with aqueous extract of
AT (AT-W) for 48 hours exhibited a dose-dependent cytotoxic effect on both H4 and CTX TNA2, but with lower effect on CTX-TNA2 cell viability. In consistent with cell viability
results, the proportion of apoptotic cells and ROS accumulation in H4 increased after
treatment with AT-W for 48 hours compared with untreated group. In conclusion, our
VII
findings clearly demonstrated that the AT-induced decrease in cell viability and cell
apoptosis follow the accumulation of ROS in H4 cells thus confirm the crucial role of
oxidative stress in AT-induced apoptosis. The mechanisms underlying this process remain to
be elucidated.
Description
Keywords
Type of access: Restricted, Artemisia.terrae-albae, glioma, apoptosis
Citation
Bayan Sailike (2022). The effect of Artemisia extracts on human astrocytoma cell viability in vitro. Nazarbayev University, Nur-sultan, Kazakhstan