STUDYING THE EFFECT OF CHONDROITIN SULFATE ON THE PHYSICOCHEMICAL PROPERTIES OF NOVEL GELATIN/CHITOSAN BIOPOLYMER-BASED CRYOGELS
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Date
2021-10-27
Authors
Kudaibergen, Gulshakhar
Zhunussova, Madina
Mun, Ellina A.
Arinova, Anar
Ogay, Vyacheslav
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI
Abstract
The application of biopolymers in tissue engineering is of a great interest due to of their
inherent properties such as cell adhesion, biodegradation, bioavailability, and viscoelasticity. In this
study, we synthesized cryogels based on biopolymers of gelatin, chitosan, and chondroitin sulfate by
cryopolymerization and studied the effect of chondroitin sulfate on changing the physicochemical
properties of cryogels such as pore size, pore volume, density, gel fraction, and biodegradation. A
macroporous surface of the synthesized polymers has been investigated by SEM. The glass transition
temperatures of the crosslinked cryogels, determined by the DSC method, were higher compared
to that of the non-crosslinked cryogel used as a reference. The results of the MTT test showed that
aqueous extracts of the prepared cryogels had no toxic effect on rat adipose-derived mesenchymal
stem cells. The research in this area is of great importance and provides new insights into novel,
effective methods for obtaining biopolymers that can be used as carriers of cells.
Description
Keywords
cryogels, gelatin, chitosan, chondroitin sulfate, biopolymers, cells, Type of access: Open Access
Citation
Kudaibergen, G.; Zhunussova, M.; Mun, E.A.; Arinova, A.; Ogay, V. Studying the Effect of Chondroitin Sulfate on the Physicochemical Properties of Novel Gelatin/Chitosan Biopolymer-Based Cryogels. Appl. Sci. 2021, 11, 10056. https://doi.org/10.3390/ app112110056