Role of ROS in AB42 mediated cell surface P-selectin expression and actin polymerization
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Date
2014
Authors
Tsoy, A.
Askarova, Sholpan
Shalakhmetova, T.
Umbayev, B.
Adambekov, S.
Zhumadilov, Z.
Journal Title
Journal ISSN
Volume Title
Publisher
Nazarbayev University
Abstract
Blood-brain barrier dysfunction plays an important role in the onset and progression
of Alzheimer's disease (AD). In the AD brains an increased deposition of A(3 in the cerebral vasculature
has been found to correlate with increased transmigration of blood-born inflammatory cells and
neurovascular inflammation [1]. Transmigration of leukocytes into brain parenchyma is a sequential
process starting with primary capture to the endothelium and rolling adhesion mediated by tethering
on selectins and selectin ligands. P-selectin is a type I transmembrane cell adhesion molecule which is
stored in cytoplasmic Weibel-Palade bodies (WPb) and can be mobilized on the endothelial cell surface
within minutes upon exposure to different pro-inflammatory agents; then it's rapidly cleared through
endocytosis in 30 min. It has become evident that A(3 activates cerebral endothelial cells (CECs) and
induces mobilization of P-selectin to the cell surface [2]. However, expression mechanisms of this receptor
on the surface of brain endothelial cells under administration with A(342 remain unclear. Since P-selectin
is stored in WPb, and there is an evidence of active role of ROS (reactive oxygen species) and actin
filaments in the different stages of WPb exocytosis, in this study we examined the dynamic of P-selectin
expression on the endothelial cell surface activated by A(342 in relation to ROS and actin polymerization.
Description
Keywords
blood-brain, leukocytes, cytoplasmic, endothelial cells, physiological trigger, actin polymerization, blood