NEUROPROTECTIVE PROPERTIES OF PLANT EXTRACT FROM LIMONIUM GMELINII
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Date
2020
Authors
Nurkenov, T.T.
Abzhanova, E.
Turgambayeva, A.
Zhusupova, G.E.
Shalakhmetova, T.M.
Tsoy, A.K.
Askarova, Sh.N.
Journal Title
Journal ISSN
Volume Title
Publisher
International conference "MODERN PERSPECTIVES FOR BIOMEDICAL SCIENCES: FROM BENCH TO BEDSIDE”; National Laboratory Astana
Abstract
logic
conditions. When excess ROS are present within the cell, this oxidative stress may have profound
deleterious effects on the cell, including the direct oxidation of biomolecules (e.g., lipid, protein, and
DNA), indirect alteration in cellular structures and functions, and the induction of cell death. The brain
is one of the organs especially vulnerable to the effects of ROS because of its high oxygen demand and
abundance of peroxidation-susceptible lipid cells. However, there are numerous data indicating that
plant polyphenols are able to reduce oxidative stress and inflammatory processes associated with brain
damage. As it was shown in previous studies, the extract of polyphenols isolated from the roots of Limonium
gmelinii (a plant widespread on the territory of Kazakhstan) has a number of therapeutic properties.
Here we investigated antioxidant and anti-inflammatory properties of polyphenols extract from L.gmelinii
in astrocytes and cerebral endothelial cells (CECs) in vitro.
Methods: Human primary astrocytes and mouse bEnd3 line of CECs were pre-treated with polyphenol
extract of L.gmelinii followed by incubation in tumor necrosis factor alpha (TNF-α) or H2O2. The level of
ROS generation, nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activation, P-selectin
expression, and activity of extracellular signal–regulated kinases 1/2(ERK1/2) were evaluated by quantitative
immunofluorescence analysis, confocal microscopy and mitogen-activated protein kinase (MAPK)
assay.
Results: It was shown that the extract of the L.gmelinii at a dosage of 30 μg/ml does not have a cytotoxic
effect. Moreover, it neutralizes the toxic effect of TNF-α by blocking the formation of ROS in astrocytes,
interfering with the activation of NADPH oxidase in astrocytes, mobilization of P-selectin on the surface
of bEnd3 cells, and inhibiting the activity of protein kinase ERK1/2.
Conclusion: It can be concluded that polyphenols extract from L.gmelinii has an antioxidant properties,
neutralizes TNF-α-induced inflammatory processes in the brain cells and could be utilized for further in
vivo examination.
Description
Keywords
endothelial cells, astrocytes, ROS, P-selectin, Limonium gmelinii, Blood-brain barrier, Research Subject Categories::MEDICINE