Sultankulov, Bolat2020-11-162020-11-162020-09-28http://nur.nu.edu.kz/handle/123456789/5097Chitosan has been a successful choice for tissue-engineering applications over the last few decades. Chitosan is a natural polysaccharide with excellent properties for tissue engineering applications, such as biodegradability, biocompatibility, and antimicrobial activity. Available free amine groups in its structure allows further chemical modifications, so new properties could be added for specific tissue engineering application. This dissertation highlights the advances made in biomaterial production and describes novel polyelectrolyte-based (PEC) cryogel that contains chitosan (CHI) and heparin (Hep). We will discuss the preparation of new cryogel material and its physico-chemical properties. Additionally, the measurement of biological activity would be addressed in vitro and in vivo. In particular, the cryogels obtained will be tested to induce differentiation of mesenchymal stem cells (rat BMSCs) derived from rat bone marrow into the osteogenic lineage. Additionally, this study will show potential uses of novel PEC-based cryogel for skin regeneration in vitro and in vivo, demonstrating the broad application of established scaffolding. The research in this dissertation is important because it demonstrates the efficacy of PEC cryogels for tissue engineering applications. This is the first PEC cryogel scaffold based on CHI-Hep made from a one-step reaction with effective loading of growth factors and cytokines.enAttribution-NonCommercial-ShareAlike 3.0 United StateschitosanCHIheparinHEPpolyelectrolyte-basedPECPhD thesis