Sultankulov, B.Berillo, D.Kauanova, S.Mikhalovsky, S.Mikhalovska, L.Saparov, A.2020-05-042020-05-042019Sultankulov, B., Berillo, D., Kauanova, S., Mikhalovsky, S., Mikhalovska, L., & Saparov, A. (2019). Composite Cryogel with Polyelectrolyte Complexes for Growth Factor Delivery. Pharmaceutics, 11(12), 650. https://doi.org/10.3390/pharmaceutics111206501999-492310.3390/pharmaceutics11120650https://www.mdpi.com/1999-4923/11/12/650https://doi.org/10.3390/pharmaceutics11120650http://nur.nu.edu.kz/handle/123456789/4596Macroporous scaffolds composed of chitosan (CHI), hydroxyapatite (HA), heparin (Hep), and polyvinyl alcohol (PVA) were prepared with a glutaraldehyde (GA) cross-linker by cryogelation. Addition of PVA to the reaction mixture slowed down the formation of a polyelectrolyte complex (PEC) between CHI and Hep, which allowed more thorough mixing, and resulted in the development of the homogeneous matrix structure. Freezing of the CHI-HA-GA and PVA-Hep-GA mixture led to the formation of a non-stoichiometric PEC between oppositely charged groups of CHI and Hep, which caused further efficient immobilization of bone morphogenic protein 2 (BMP-2) possible due to electrostatic interactions. It was shown that the obtained cryogel matrix released BMP-2 and supported the differentiation of rat bone marrow mesenchymal stem cells (rat BMSCs) into the osteogenic lineage. Rat BMSCs attached to cryogel loaded with BMP-2 and expressed osteocalcin in vitro. Obtained composite cryogel with PEC may have high potential for bone regeneration and tissue engineering applications.enAttribution-NonCommercial-ShareAlike 3.0 United Statesbiomaterialscryogeldrug deliverygrowth factorstissue engineeringbone regenerationResearch Subject Categories::MEDICINEArticle