Kurmanova, Asiya2024-06-252024-06-252024-04-26Kurmanova, A. (2024). Role of Periostin in Rheumatoid Arthritis. Nazarbayev University School of Medicinehttp://nur.nu.edu.kz/handle/123456789/7996Rheumatoid Arthritis (RA) is defined as an autoimmune disease of chronic character that principally leads to inflammation in synovial joints. Periostin belongs to the extracellular matrix protein family. It plays a role in ontogenesis and is produced in adult tissues subjected to mechanical forces inclusive of bones and joints. It has been shown to have increased expression in the synovium of patients with RA (Kasperovitz et al., 2005). In research by Chijimatsu et al. (2015), human primary chondrocytes expressed interleukin (IL)-6, IL-8, IL-1, matrix metalloproteinase (MMP)-1, MMP-3, MMP-13, as well as nitric oxide synthase-2 (NOS2) in response to periostin. This suggests that periostin may amplify inflammatory events. Per contra, in the results demonstrated by Cheon (2017), mean histologic severity scores were elevated in periostin-deficient mice compared to wild type implying a protective role of periostin in RA. Considering the contradictory results of the abovementioned studies and the lack of information regarding the presence of periostin splice variants, further investigations are needed. This project examines periostin's anti-inflammatory role in a mouse RA model. In this study, 4 wild type and 2 periostin-KO underwent immunization injections to establish a collagen-induced arthritis (CIA) mouse model (Brand et al., 2007). The effect was evaluated observationally, by taking weight and hind paw measurements, histologically and immunohistochemically by staining for periostin and RAR-related orphan receptor gamma (RORγ).enResearch Subject Categories::MEDICINERheumatoid ArthritisMouse ModelCIAPeriostinC57BL/6RAR-related orphan receptor gammaRORγTh17Type of access: EmbargoMaster's thesis