Daniyarov, AssetMolkenov, AskhatRakhimova, SauleAkhmetova, AinurNurkina, ZhannurYerezhepov, DaurenChingissova, LyailyaBismilda, VeneraToxanbaeva, BekzatAkilzhanova, AinurKozhamkulov, UlanKairov, Ulykbek2021-01-272021-01-272020-12Daniyarov, A., Molkenov, A., Rakhimova, S., Akhmetova, A., Nurkina, Z., Yerezhepov, D., Chingissova, L., Bismilda, V., Toxanbaeva, B., Akilzhanova, A., Kozhamkulov, U., & Kairov, U. (2020). Whole genome sequence data of Mycobacterium tuberculosis XDR strain, isolated from patient in Kazakhstan. Data in Brief, 33, 106416. https://doi.org/10.1016/j.dib.2020.1064162352-3409https://www.sciencedirect.com/science/article/pii/S2352340920312981?via%3Dihubhttps://doi.org/10.1016/j.dib.2020.106416http://nur.nu.edu.kz/handle/123456789/5251Drug-resistant tuberculosis (TB) is a major public health problem. Clinical Mycobacterium tuberculosis (MTB) isolate with Extensively drug-resistant tuberculosis (MTB-XDR) profile was subjected to whole-genome sequencing using a next-generation sequencing platform (NGS) Roche 454 GS FLX+ followed by bioinformatics sequence analysis. Quality of read was checked by FastQC, paired-end reads were trimmed using Trimmomatic. De novo genome assembly was conducted using Velvet v.1.2.10. The assembled genome of XDR-TB-1599 strain was functionally annotated using the PATRIC platform. Analysis of de novo assembled genome was performed using ResFinder, CARD, CASTB and TB-Profiler tools. MIRU_VNTR genotyping on 12 loci and spoligotyping have been performed for XDR-TB-1599 isolate. M. tuberculosis XDR-TB-1599 strain yielded an average read depth of 21-fold with overall 4 199 325 bp. The assembled genome contains 5528 protein-coding genes, including key drug resistance and virulence-associated genes and GC content of 65.4%. We identified that all proteins encoded by this strain contain conserved domains associated with the first-line anti-tuberculosis drugs such as rifampicin, isoniazid, streptomycin and ethionamide. TB-Profiler had higher average concordance results with phenotypic DST (drug susceptibility testing) in comparison with ResFinder, CARD, CASTB profiling to first-line (75% vs 50%) and second-line (25% vs 0%) of anti-TB drugs, correspondingly. To our knowledge, this is the first report of a highly annotated and characterized whole-genome sequence and de novo assembled XDR-TB M.tuberculosis strain isolated from a sputum of new TB case-patient from Kazakhstan performed on Roche 454 GS FLX+ platform. This report highlights an important role of whole-genome sequencing technology and analysis as an advanced approach for drug-resistance investigations of circulated TB isolates.enAttribution-NonCommercial-ShareAlike 3.0 United StatesMycobacteriumWhole genome sequenceDraft genomeExtensively drug-resistanceTuberculosisKazakhstanResearch Subject Categories::NATURAL SCIENCESResearch Subject Categories::NATURAL SCIENCES::BiologyArticle