Irgaliyev, Abdyrakhman2024-11-272024-11-272024Irgaliyev, A. (2024). LSP project report Development of HepG cell line with human NTCP gene. Nazarbayev University School of Medicinehttps://nur.nu.edu.kz/handle/123456789/8322Hepatitis B virus (HBV) is one of the most common viral infections of the liver. There are 296 million people around the world with chronic hepatitis B and 1.5 million new cases emerge each year (WHO on Hepatitis B, 2021). Also, complications of hepatitis B virus such as liver cirrhosis and hepatocellular carcinoma were the cause of 820 000 deaths in 2019. It creates a significant economic and social burden, especially in developing countries. Until the 2010s the proper mechanism of viral internalization of hepatitis B virus was not properly understood. However, discovery of Na+-taurocholate cotransporting polypeptide (NTCP) protein allowed better understanding of the process as well as new possibilities in treatment. Common anti-HBV treatment is usually antiretroviral utilizing its usage of reverse transcriptase. The discovery of a specific viral entry point may allow development of new treatment options. However, expression of NTCP is downregulated in in vitro hepatocytes. This poses an obstacle in creating a testing field for medication options. Sadly, during this research the results were not achieved due to shortage of time and difficulties with competent cells.enAttribution-NonCommercial-ShareAlike 3.0 United StatesType of access:Hepatitis B virusMaster`s thesis