González-Plaza, Juan JoséGutiérrez-Repiso, CarolinaGarcía-Serrano, SaraRodriguez-Pacheco, FranciscaGarrido-Sánchez, LourdesSantiago-Fernández, ConcepciónGarcía-Arnés, JuanMoreno-Ruiz, Francisco J.Rodríguez-Cañete, AlbertoGarcía-Fuentes, Eduardo2018-01-042018-01-042016-02-01Juan José González-Plaza, Carolina Gutiérrez-Repiso, Sara García-Serrano, Francisca Rodriguez-Pacheco, Lourdes Garrido-Sánchez, Concepción Santiago-Fernández, Juan García-Arnés, Francisco J. Moreno-Ruiz, Alberto Rodríguez-Cañete, Eduardo García-Fuentes, Effect of Roux-en-Y gastric bypass-induced weight loss on the transcriptomic profiling of subcutaneous adipose tissue, In Surgery for Obesity and Related Diseases, Volume 12, Issue 2, 2016, Pages 257-26315507289https://www.sciencedirect.com/science/article/pii/S1550728915002920http://nur.nu.edu.kz/handle/123456789/3108Abstract BackgroundThe changes in the transcriptomic profiling of subcutaneous adipose tissue (SAT) when weight loss stabilizes after a Roux-en-Y gastric bypass (RYGB) are still largely unknown. ObjectivesTo investigate the changes produced in SAT gene expression of morbidly obese women when their weight loss stabilizes 2 years after RYGB. SettingUniversity hospital. MethodsSAT biopsies of the periumbilical area were taken before and 2 years after RYGB. Gene expression levels were assessed by microarray analysis and significant differences in gene expression were validated by real-time quantitative polymerase chain reaction. The findings were also confirmed in an independent population of morbidly obese women. ResultsMicroarray analysis revealed that the overexpressed differentially expressed genes have a prominent role in the pathways involved in biosynthetic processes, especially lipid or carboxylic ones (stearoyl-Coenzyme A desaturase-1, fatty acid desaturase-1, fatty acid elongase-6, ATP citrate lyase, fatty acid synthase, lipin-1, monoacylglycerol O-acyltransferase, patatin-like phospholipase domain containing-3, phosphate cytidylyltransferase-2, cholesteryl ester transfer protein, transmembrane 7 superfamily member 2, pyruvate carboxylase, and glycogen synthase 2). Most of the underexpressed differentially expressed genes are related with immune system and inflammation processes (immune responses, response to stress, cell death, regulation of biological quality, immune effector process, the response to endogenous stimulus, and the response to other types of stimulus). ConclusionAn improvement of the SAT inflammatory and immune profile and an induction of genes involved in the regulation of lipid metabolism are shown when weight loss stabilizes 2 years after RYGB. Most of the genes shown are clearly linked to obesity and other metabolic disorders.TranscriptomicsMicroarrayBariatric surgeryGastric bypassSubcutaneous adipose tissueMorbid obesityFunctional annotationExpression analysisArticleCopyright © 2016 American Society for Bariatric Surgery. Published by Elsevier Inc. All rights reserved.