Issatay, Aigerim2024-11-272024-11-272024Issatay, A. (2024). Characterizing the role of CTHRC 1 as a biomarker for synovial fluid in Rheumatoid arthritis and its potential implications in disease pathogenesis. Nazarbayev University School of Medicinehttps://nur.nu.edu.kz/handle/123456789/8312Rheumatoid arthritis (RA) is a complex autoimmune disease characterized by multifactorial etiology, which includes environmental, genetic, and immunological factors. Early RA detection and assessing its activity remains a challenging issue due to the diverse nature of the disease. Currently, available diagnostic protocols include measurement of rheumatoid factor (RF) and anti-cyclic citrullinated protein( ACCP). However, their effectiveness is hindered due to their moderate sensitivity and specificity. Moreover, they are not detected in the early disease course, leading to complications and substantial health burden. This paper explores the potential of Collagen Triple Helix Repeat Containing 1 (CTHRC1) as a novel biomarker for RA. We measured plasma CTHRC 1 in the plasma of RA (n=30) and OA (n=29) patients and identified statistical differences. Plasma of CTHRC 1 is significantly elevated in RA patients as compared to OA. Immunofluorescence was done to observe the expression of different markers involved in the pathogenesis of RA disease. Additionally, potential biomarkers, such as Periostin and Cartilage oligomeric matrix protein (COMP) were also evaluated in this study, their expression and role in differentiation between RA and OA patients. The results of the study may play a significant role in future diagnosis of RA since CTHRC 1 is an easy-to-measure and available diagnostic tool.enAttribution-NonCommercial-ShareAlike 3.0 United StatesType of access:Rheumatoid arthritisMaster`s thesis