Yoo, Jung SunKim, Hong BaeOgay, VyacheslavLee, Byung-CheonAhn, SaeyoungSoh, Kwang-sup2016-02-292016-02-292009-04-01http://nur.nu.edu.kz/handle/123456789/1330Objective: The present study has been designed to find a possible new route for the metastasis of cancer cells on the fascia surrounding tumor tissue using a novel technique of trypan blue staining. Materials and Methods: Tumor tissues were grown in the skin of nude mice after subcutaneous inoculation with human lung cancer cells. Trypan blue was recently identified as a dye with specificity for Bonghan ducts (BHDs) and not other tissues, such as blood or lymph vessels or nerves. Results: We demonstrate that the trypan blue staining technique allows the first visualization of BHDs which are connected to tumor tissues.enAttribution-NonCommercial-ShareAlike 3.0 United StatesBonghan ductcancermetastasistrypan blueResearch Subject Categories::MEDICINE::Microbiology, immunology, infectious diseases::MicrobiologyArticle