Siddiqui, SammerAnwar, Muhammad F.Naeem, SadafAbidi, Syed HaniZarina, ShamshadAli, Syed2020-05-192020-05-192018-02-28Siddiqui, S., Anwar, M. F., Naeem, S., Abidi, S. H., Zarina, S., & Ali, S. (2018). Simian Virus 40 Large T Antigen as a Model to Test the Efficacy of Flouroquinolones against Viral Helicases. Bioinformation, 14(2), 75–79. https://doi.org/10.6026/973206300140750973-206310.6026/97320630014075http://www.bioinformation.net/014/97320630014075.htmhttps://doi.org/10.6026/97320630014075http://nur.nu.edu.kz/handle/123456789/4735Simian virus 40 large T-antigen (SV40 LT-Ag) is a 708 amino acid nuclear phosphoprotein. Among many functions of LT-Ag is its ability to perform as an ATPase-helicase, catalyzing the unwinding of viral genome during replication. The LT-Ag has been employed in the studies of helicase structure and function, and has served as a model helicase for the screening of antiviral drugs that target viral helicase. In this study, using in vitro enzyme assays and in silico computer modeling, we screened a batch of 18 fluoroquinolones to assess their potential as antivirals by virtue of their inhibition of the LT-Ag helicase. We found all fluoroquinolones to be inhibitory to the helicase activity of LT-Ag. In our docking analysis, most of these tested drugs showed similarity in their interactions with LT-Ag. Our study shows the potential of fluoroquinolones as antiviral drugs and of SV40 LT-Ag as a model protein for screening drugs against viral helicases.enAttribution-NonCommercial-ShareAlike 3.0 United StatesSV40 LT-AgFluoroquinolonesAntiviral drugsViral HelicasesResearch Subject Categories::MEDICINEArticle