Sun, XiaomingShi, YiAkahoshi, TomohiroFujiwara, MamoruGatanaga, HiroyukiSchönbach, ChristianKuse, NozomiAppay, VictorGao, George F.Oka, ShinichiTakiguchi, Masafumi2018-01-052018-01-052016-06-07Xiaoming Sun, Yi Shi, Tomohiro Akahoshi, Mamoru Fujiwara, Hiroyuki Gatanaga, Christian Schönbach, Nozomi Kuse, Victor Appay, George F. Gao, Shinichi Oka, Masafumi Takiguchi, Effects of a Single Escape Mutation on T Cell and HIV-1 Co-adaptation, In Cell Reports, Volume 15, Issue 10, 2016, Pages 2279-229122111247https://www.sciencedirect.com/science/article/pii/S221112471630585Xhttp://nur.nu.edu.kz/handle/123456789/3113Summary The mechanistic basis for the progressive accumulation of Y135F Nef mutant viruses in the HIV-1-infected population remains poorly understood. Y135F viruses carry the 2F mutation within RW8 and RF10, which are two HLA-A∗24:02-restricted superimposed Nef epitopes recognized by distinct and adaptable CD8+ T cell responses. We combined comprehensive analysis of the T cell receptor repertoire and cross-reactive potential of wild-type or 2F RW8- and RF10-specific CD8+ T cells with peptide-MHC complex stability and crystal structure studies. We find that, by affecting direct and water-mediated hydrogen bond networks within the peptide-MHC complex, the 2F mutation reduces both TCR and HLA binding. This suggests an advantage underlying the evolution of the 2F variant with decreased CD8+ T cell efficacy. Our study provides a refined understanding of HIV-1 and CD8+ T cell co-adaptation at the population level.enArticle© 2016 The Authors.