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Analysis of protector mechanism of probiotics using DNA comet assay

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dc.contributor.author Zhulamanova, A.
dc.contributor.author Shakhabayeva, G.
dc.contributor.author Saduakhasova, S.
dc.contributor.author Kozhakhmetov, S.
dc.contributor.author Kushugulova, A.
dc.date.accessioned 2015-10-28T08:50:26Z
dc.date.available 2015-10-28T08:50:26Z
dc.date.issued 2014
dc.identifier.isbn 9786018046728
dc.identifier.uri http://nur.nu.edu.kz/handle/123456789/553
dc.description.abstract Ecology affects human organism by causing breaks in DNA and, therefore, induce mutations and malignant cell formation. Comet assay is a quantitative method that allows to measure DNA damage in eukaryotic cells (Liao, McNutt, & Zhu, 2009). This method is widely used in areas, such as human biomonitoring, genotoxicology, and ecology. Comet assay is also called a single-cell gel electrophoresis. Thus, the products of electrophoresis are analyzed under the fluorescent light, which makes it similar to comets. DNA strand breaks are detected by comparing the intensity of the comet tail relative to the head (Collins, 2004). Probiotics containing Lactobacillus rhamnosus were used, as Lactobacilli probiotics were found to be safe and effective against urogenital diseases, food hypersensitivity, and dental caries (Lebeer, Vanderleyden, & De Keersmaeckler, 2008). The aim of the research was to study DNA protector mechanism for three probiotic products, containing L. rhamnosus. ru_RU
dc.language.iso en ru_RU
dc.publisher Nazarbayev University ru_RU
dc.subject ecology ru_RU
dc.subject human organism ru_RU
dc.subject DNA ru_RU
dc.subject comet assay ru_RU
dc.subject eukaryotic cells ru_RU
dc.subject biomonitoring ru_RU
dc.subject genotoxicology ru_RU
dc.subject gel electrophoresis ru_RU
dc.subject electrophoresis ru_RU
dc.subject fluorescent light ru_RU
dc.subject probiotics ru_RU
dc.title Analysis of protector mechanism of probiotics using DNA comet assay ru_RU
dc.type Abstract ru_RU


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