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ZEB1 and IL-6/11-STAT3 signalling cooperate to define invasive potential of pancreatic cancer cells via differential regulation of the expression of S100 proteins

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dc.contributor.author Al-Ismaeel, Qais
dc.contributor.author Neal, Christopher P.
dc.contributor.author Al-Mahmoodi, Hanaa
dc.contributor.author Almutairi, Zamzam
dc.contributor.author Al-Shamarti, Ibtihal
dc.contributor.author Straatman, Kees
dc.contributor.author Jaunbocus, Nabil
dc.contributor.author Irvine, Andrew
dc.contributor.author Issa, Eyad
dc.contributor.author Moreman, Catherine
dc.contributor.author Dennison, Ashley R.
dc.contributor.author Sayan, A. Emre
dc.contributor.author McDearmid, Jonathan
dc.contributor.author Greaves, Peter
dc.contributor.author Tulchinsky, Eugene
dc.contributor.author Kriajevska, Marina
dc.date.accessioned 2019-12-11T05:46:01Z
dc.date.available 2019-12-11T05:46:01Z
dc.date.issued 2019-07-02
dc.identifier.citation Al-Ismaeel, Q., Neal, C. P., Al-Mahmoodi, H., Almutairi, Z., Al-Shamarti, I., Straatman, K., … Kriajevska, M. (2019). ZEB1 and IL-6/11-STAT3 signalling cooperate to define invasive potential of pancreatic cancer cells via differential regulation of the expression of S100 proteins. British Journal of Cancer, 121(1), 65–75. https://doi.org/10.1038/s41416-019-0483-9 en_US
dc.identifier.other 000473525700009
dc.identifier.uri http://nur.nu.edu.kz/handle/123456789/4356
dc.description https://www.nature.com/articles/s41416-019-0483-9#Abs1 en_US
dc.description.abstract Background S100 proteins have been implicated in various aspects of cancer, including epithelial-mesenchymal transitions (EMT), invasion and metastasis, and also in inflammatory disorders. Here we examined the impact of individual members of this family on the invasion of pancreatic ductal adenocarcinoma (PDAC) cells, and their regulation by EMT and inflammation. Methods Invasion of PDAC cells was analysed in zebrafish embryo xenografts and in transwell invasion assays. Expression and regulation of S100 proteins was studied in vitro by immunoblotting, quantitative PCR and immunofluorescence, and in pancreatic lesions by immunohistochemistry. Results Whereas the expression of most S100 proteins is characteristic for epithelial PDAC cell lines, S100A4 and S100A6 are strongly expressed in mesenchymal cells and upregulated by ZEB1. S100A4/A6 and epithelial protein S100A14 respectively promote and represses cell invasion. IL-6/11-STAT3 pathway stimulates expression of most S100 proteins. ZEB1 synergises with IL-6/11-STAT3 to upregulate S100A4/A6, but nullifies the effect of inflammation on S100A14 expression. Conclusion EMT/ZEB1 and IL-6/11-STAT3 signalling act independently and congregate to establish the expression pattern of S100 proteins, which drives invasion. Although ZEB1 regulates expression of S100 family members, these effects are masked by IL-6/11-STAT3 signalling, and S100 proteins cannot be considered as bona fide EMT markers in PDAC. en_US
dc.language.iso en en_US
dc.publisher NATURE PUBLISHING GROUP en_US
dc.rights Attribution-NonCommercial-ShareAlike 3.0 United States *
dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/3.0/us/ *
dc.subject pancreatic ductal adenocarcinoma en_US
dc.subject PDAC en_US
dc.subject ZEB1 en_US
dc.subject IL-6/11-STAT3 en_US
dc.subject pancreatic cancer cells en_US
dc.subject S100 proteins en_US
dc.title ZEB1 and IL-6/11-STAT3 signalling cooperate to define invasive potential of pancreatic cancer cells via differential regulation of the expression of S100 proteins en_US
dc.type Article en_US
workflow.import.source science


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