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Histological and biochemical analysis of DNA damage after BNCT in rat model

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dc.contributor.author Masutani, Mitsuko
dc.contributor.author Baiseitov, Diaz
dc.contributor.author Itoh, Tasuku
dc.contributor.author Hirai, Takahisa
dc.contributor.author Berikkhanova, Kulzhan
dc.contributor.author Murakami, Yasufumi
dc.contributor.author Zhumadilov, Zhaxybay
dc.contributor.author Imahori, Yoshio
dc.contributor.author Hoshi, Masaharu
dc.contributor.author Itami, Jun
dc.creator Mitsuko, Masutani
dc.date.accessioned 2018-01-04T08:57:35Z
dc.date.available 2018-01-04T08:57:35Z
dc.date.issued 2014-06-01
dc.identifier DOI:10.1016/j.apradiso.2014.03.003
dc.identifier.citation Mitsuko Masutani, Diaz Baiseitov, Tasuku Itoh, Takahisa Hirai, Kulzhan Berikkhanova, Yasufumi Murakami, Zhaxybay Zhumadilov, Yoshio Imahori, Masaharu Hoshi, Jun Itami, Histological and biochemical analysis of DNA damage after BNCT in rat model, In Applied Radiation and Isotopes, Volume 88, 2014, Pages 104-108 en_US
dc.identifier.issn 09698043
dc.identifier.uri https://www.sciencedirect.com/science/article/pii/S0969804314000803
dc.identifier.uri http://nur.nu.edu.kz/handle/123456789/3094
dc.description.abstract Abstract To understand the mechanism of tumor cell death induced by boron neutron capture therapy (BNCT) and to optimize BNCT condition, we used rat tumor graft models and histological and biochemical analyses were carried out focusing on DNA damage response. Rat lymphosarcoma cells were grafted subcutaneously into male Wister rats. The rats with developed tumors were then treated with neutron beam irradiation 45min after injection of 330mg/kg bodyweight boronophenylalanine (10BPA) (+BPA) or saline control (–BPA). BNCT was carried out in the National Nuclear Center of the Republic of Kazakhstan (neutron flux: 1×109nvt/s, fluence: 6×1011nvt) with the presence of background γ-irradiation of 33Gy. 6 and 20h after BNCT treatment, tumors were resected, fixed and subjected to immunohistochemistry and biochemical analyses. Immunostaining of nuclei showed that double strand break (DSB) marker gamma H2AX staining was high in 20h/+BPA sample but not in 20h/–BPA samples. Poly(ADP-ribose), DSB and single strand break markers of DNA, also demonstrated this tendency. These two markers were observed at low levels in unirradiated tissues or 6h after BNCT either under −BPA and +BPA conditions. HMGB1 level increased in 6h/+BPA but not in 6h/−BPA or 20h/+BPA samples. The persistent staining of γH2AX and poly(ADP-ribose) in +BPA group suggests accumulated DSB damage after BNCT. The early HMGB1 upregulation and γH2AX and poly(ADP-ribose) observed later might be the markers for monitoring the DNA damage induced by BNCT. en_US
dc.language.iso en en_US
dc.publisher Applied Radiation and Isotopes en_US
dc.relation.ispartof Applied Radiation and Isotopes
dc.subject BNCT en_US
dc.subject DNA damage response en_US
dc.subject BPA en_US
dc.subject γH2AX en_US
dc.subject PAR en_US
dc.subject HMGB1 en_US
dc.title Histological and biochemical analysis of DNA damage after BNCT in rat model en_US
dc.type Article en_US
dc.rights.license Copyright © 2014 Elsevier Ltd. All rights reserved.
elsevier.identifier.doi 10.1016/j.apradiso.2014.03.003
elsevier.identifier.eid 1-s2.0-S0969804314000803
elsevier.identifier.pii S0969-8043(14)00080-3
elsevier.identifier.scopusid 84901836830
elsevier.identifier.pubmedid 24690552
elsevier.volume 88
elsevier.issue.name 15th International Congress on Neutron Capture Therapy Impact of a new radiotherapy against cancer
elsevier.coverdate 2014-06-01
elsevier.coverdisplaydate June 2014
elsevier.startingpage 104
elsevier.endingpage 108
elsevier.openaccess 0
elsevier.openaccessarticle false
elsevier.openarchivearticle false
elsevier.teaser To understand the mechanism of tumor cell death induced by boron neutron capture therapy (BNCT) and to optimize BNCT condition, we used rat tumor graft models and histological and biochemical analyses...
elsevier.aggregationtype Journal
workflow.import.source science


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