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The in vitro characterization of a gelatin scaffold, prepared by cryogelation and assessed in vivo as a dermal replacement in wound repair

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dc.contributor.author Shevchenko, Rostislav V.
dc.contributor.author Eeman, Marc
dc.contributor.author Rowshanravan, Behzad
dc.contributor.author Allan, Iain U.
dc.contributor.author Savina, Irina N.
dc.contributor.author Illsley, Matt
dc.contributor.author Salmon, Michel
dc.contributor.author James, Stuart L.
dc.contributor.author Mikhalovsky, Sergey V.
dc.contributor.author James, S. Elizabeth
dc.creator Rostislav V., Shevchenko
dc.date.accessioned 2017-12-26T10:01:49Z
dc.date.available 2017-12-26T10:01:49Z
dc.date.issued 2014-07-01
dc.identifier DOI:10.1016/j.actbio.2014.03.027
dc.identifier.citation Rostislav V. Shevchenko, Marc Eeman, Behzad Rowshanravan, Iain U. Allan, Irina N. Savina, Matt Illsley, Michel Salmon, Stuart L. James, Sergey V. Mikhalovsky, S. Elizabeth James, The in vitro characterization of a gelatin scaffold, prepared by cryogelation and assessed in vivo as a dermal replacement in wound repair, In Acta Biomaterialia, Volume 10, Issue 7, 2014, Pages 3156-3166 en_US
dc.identifier.issn 17427061
dc.identifier.uri https://www.sciencedirect.com/science/article/pii/S1742706114001470
dc.identifier.uri http://nur.nu.edu.kz/handle/123456789/3066
dc.description.abstract Abstract A sheet gelatin scaffold with attached silicone pseudoepidermal layer for wound repair purposes was produced by a cryogelation technique. The resulting scaffold possessed an interconnected macroporous structure with a pore size distribution of 131±17μm at one surface decreasing to 30±8μm at the attached silicone surface. The dynamic storage modulus (G′) and mechanical stability were comparable to the clinical gold standard dermal regeneration template, Integra®. The scaffolds were seeded in vitro with human primary dermal fibroblasts. The gelatin based material was not only non-cytotoxic, but over a 28day culture period also demonstrated advantages in cell migration, proliferation and distribution within the matrix when compared with Integra®. When seeded with human keratinocytes, the neoepidermal layer that formed over the cryogel scaffold appeared to be more advanced and mature when compared with that formed over Integra®. The in vivo application of the gelatin scaffold in a porcine wound healing model showed that the material supports wound healing by allowing host cellular infiltration, biointegration and remodelling. The results of our in vitro and in vivo studies suggest that the gelatin based scaffold produced by a cryogelation technique is a promising material for dermal substitution, wound healing and other potential biomedical applications. en_US
dc.language.iso en en_US
dc.publisher Acta Biomaterialia en_US
dc.relation.ispartof Acta Biomaterialia
dc.subject Cryogel en_US
dc.subject Macroporous scaffold en_US
dc.subject Wound healing en_US
dc.subject Dermal skin substitute en_US
dc.subject Pig model en_US
dc.title The in vitro characterization of a gelatin scaffold, prepared by cryogelation and assessed in vivo as a dermal replacement in wound repair en_US
dc.type Article en_US
dc.rights.license Copyright © 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
elsevier.identifier.doi 10.1016/j.actbio.2014.03.027
elsevier.identifier.eid 1-s2.0-S1742706114001470
elsevier.identifier.pii S1742-7061(14)00147-0
elsevier.identifier.scopusid 84901792360
elsevier.identifier.pubmedid 24704695
elsevier.volume 10
elsevier.issue.identifier 7
elsevier.coverdate 2014-07-01
elsevier.coverdisplaydate July 2014
elsevier.startingpage 3156
elsevier.endingpage 3166
elsevier.openaccess 0
elsevier.openaccessarticle false
elsevier.openarchivearticle false
elsevier.teaser A sheet gelatin scaffold with attached silicone pseudoepidermal layer for wound repair purposes was produced by a cryogelation technique. The resulting scaffold possessed an interconnected macroporous...
elsevier.aggregationtype Journal
workflow.import.source science


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