dc.contributor.author |
Shevchenko, Rostislav V.
|
|
dc.contributor.author |
Eeman, Marc
|
|
dc.contributor.author |
Rowshanravan, Behzad
|
|
dc.contributor.author |
Allan, Iain U.
|
|
dc.contributor.author |
Savina, Irina N.
|
|
dc.contributor.author |
Illsley, Matt
|
|
dc.contributor.author |
Salmon, Michel
|
|
dc.contributor.author |
James, Stuart L.
|
|
dc.contributor.author |
Mikhalovsky, Sergey V.
|
|
dc.contributor.author |
James, S. Elizabeth
|
|
dc.creator |
Rostislav V., Shevchenko |
|
dc.date.accessioned |
2017-12-26T10:01:49Z |
|
dc.date.available |
2017-12-26T10:01:49Z |
|
dc.date.issued |
2014-07-01 |
|
dc.identifier |
DOI:10.1016/j.actbio.2014.03.027 |
|
dc.identifier.citation |
Rostislav V. Shevchenko, Marc Eeman, Behzad Rowshanravan, Iain U. Allan, Irina N. Savina, Matt Illsley, Michel Salmon, Stuart L. James, Sergey V. Mikhalovsky, S. Elizabeth James, The in vitro characterization of a gelatin scaffold, prepared by cryogelation and assessed in vivo as a dermal replacement in wound repair, In Acta Biomaterialia, Volume 10, Issue 7, 2014, Pages 3156-3166 |
en_US |
dc.identifier.issn |
17427061 |
|
dc.identifier.uri |
https://www.sciencedirect.com/science/article/pii/S1742706114001470 |
|
dc.identifier.uri |
http://nur.nu.edu.kz/handle/123456789/3066 |
|
dc.description.abstract |
Abstract A sheet gelatin scaffold with attached silicone pseudoepidermal layer for wound repair purposes was produced by a cryogelation technique. The resulting scaffold possessed an interconnected macroporous structure with a pore size distribution of 131±17μm at one surface decreasing to 30±8μm at the attached silicone surface. The dynamic storage modulus (G′) and mechanical stability were comparable to the clinical gold standard dermal regeneration template, Integra®. The scaffolds were seeded in vitro with human primary dermal fibroblasts. The gelatin based material was not only non-cytotoxic, but over a 28day culture period also demonstrated advantages in cell migration, proliferation and distribution within the matrix when compared with Integra®. When seeded with human keratinocytes, the neoepidermal layer that formed over the cryogel scaffold appeared to be more advanced and mature when compared with that formed over Integra®. The in vivo application of the gelatin scaffold in a porcine wound healing model showed that the material supports wound healing by allowing host cellular infiltration, biointegration and remodelling. The results of our in vitro and in vivo studies suggest that the gelatin based scaffold produced by a cryogelation technique is a promising material for dermal substitution, wound healing and other potential biomedical applications. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Acta Biomaterialia |
en_US |
dc.relation.ispartof |
Acta Biomaterialia |
|
dc.subject |
Cryogel |
en_US |
dc.subject |
Macroporous scaffold |
en_US |
dc.subject |
Wound healing |
en_US |
dc.subject |
Dermal skin substitute |
en_US |
dc.subject |
Pig model |
en_US |
dc.title |
The in vitro characterization of a gelatin scaffold, prepared by cryogelation and assessed in vivo as a dermal replacement in wound repair |
en_US |
dc.type |
Article |
en_US |
dc.rights.license |
Copyright © 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. |
|
elsevier.identifier.doi |
10.1016/j.actbio.2014.03.027 |
|
elsevier.identifier.eid |
1-s2.0-S1742706114001470 |
|
elsevier.identifier.pii |
S1742-7061(14)00147-0 |
|
elsevier.identifier.scopusid |
84901792360 |
|
elsevier.identifier.pubmedid |
24704695 |
|
elsevier.volume |
10 |
|
elsevier.issue.identifier |
7 |
|
elsevier.coverdate |
2014-07-01 |
|
elsevier.coverdisplaydate |
July 2014 |
|
elsevier.startingpage |
3156 |
|
elsevier.endingpage |
3166 |
|
elsevier.openaccess |
0 |
|
elsevier.openaccessarticle |
false |
|
elsevier.openarchivearticle |
false |
|
elsevier.teaser |
A sheet gelatin scaffold with attached silicone pseudoepidermal layer for wound repair purposes was produced by a cryogelation technique. The resulting scaffold possessed an interconnected macroporous... |
|
elsevier.aggregationtype |
Journal |
|
workflow.import.source |
science |
|