Abstracts
http://nur.nu.edu.kz:80/handle/123456789/5089
2024-03-29T09:20:05ZMOUSE CYTOKINE-INDUCED KILLER CELLS DEVELOPED FROM DIFFERENT SOURCES
http://nur.nu.edu.kz:80/handle/123456789/5192
MOUSE CYTOKINE-INDUCED KILLER CELLS DEVELOPED FROM DIFFERENT SOURCES
Issabekova, A.; Zhunussova, M.; Zhumabekova, M.; Ogay, V.
Introduction: In Kazakhstan, death from colorectal cancer is on the leading position among cancer-related
deaths in the population, and since 2013 colorectal cancer is one of the three cancer diseases subject
to the National Screening Program. The treatment protocol used for colorectal cancer therapy with
metastases has very low efficacy. Another strategy in cancer therapy is immunotherapy with cytokine-induced
killer cells (CIK cells). Human CIK cells are isolated from peripheral blood mononuclear cell fraction
using IFN-γ, IL-2cytokine and anti-CD3 monoclonal antibodies. As a result, a heterogenous population
which consists mainly of CD3+CD56-, CD3+CD56+ cells and of a small population of CD3-CD56+cells is
obtained. Among the killer cells obtained, CD3+CD56+ have the greatest cytotoxic activity. For developed
preclinical studies of CIK cells in murine model we search the best source of CIK cells within spleen,
lymph nodes, bone marrow.
Methods: CIK cells will be proliferated from mouse spleen, lymph nodes, bone marrow cells. Spleen,
lymph nodes, bone marrow cells without monocytes and erythrocytes expanded with IFN-γ, IL-2cytokines
and anti-CD3 monoclonal antibodies for 14 days. Positive selection of CIK cells against NK1.1and
DX5 will be performed on immune beads (Miltenyi biotech).
Results: CIK cells are characterized by both MHC-restricted and MHC-unrestricted anti-tumor cytotoxicity
against a broad range of cancer cells. Mouse CIK cells have distinct phenotype from human CIK cells.
NK1.1and DX5 are murine natural killer markers. According to literature data after culturing spleen cells
for 21day NK1.1+ and DX5+ of TCRαβ+ CD3+ CD8+ T cells have the greatest cytotoxicity. We evaluated
NK1.1+ and DX5+ cells after culturing cells isolated from spleen, lymph nodes and bone marrow for 14
days. NK1.1positive cells were 53,3% and DX5+ were 5% from bone marrow cells, but bone marrow cells
showed low amounts of expanded cells. 21,8% of spleen cells showed NK1.1+ phenotype, 20% of DX5
(CD49b). Lymph nodes gave rise to 12,3% NK1.1+ cells. According to proliferation potential and portion
of NK1.1+ and DX5+, spleen and lymph nodes are prospective sources of CIK cells.
Conclusion: Spleen and lymph node cells may be sources for expansion of mouse CIK cells.
2020-01-01T00:00:00ZHEREDITARY DISEASES AND CONGENITAL MALFORMATIONS REGISTRATION AND MONITORING INFORMATION SYSTEM
http://nur.nu.edu.kz:80/handle/123456789/5191
HEREDITARY DISEASES AND CONGENITAL MALFORMATIONS REGISTRATION AND MONITORING INFORMATION SYSTEM
Nazarova, L.; Tolegen, N.; Bayanova, M.
Introduction. Database on hereditary diseases is one of the main tasks of healthcare. Register of hereditary
pathology allows to assess accurately the frequency of childbirths with various hereditary diseases
and congenital malformations in the population. The purpose of this work is to present the frequency
and structure of congenital and hereditary pathology based on the use of the original genetic database
for monitoring of congenital malformations and hereditary diseases.
Methods: Clinical-genealogical, cytogenetic, FISH method, molecular-genetic (MLPA, mass spectrometry
and fluorimetry, Sanger sequencing, NGS), statistical.
Results: Up to date, National Research Center for Maternal and Child Health’s genetic register database
has registered 4564 patients and fetuses with congenital and hereditary pathologies. Congenital malformations
prevail in the database with 2317 cases (50,8%), including congenital malfunctions of obligatory
registration according to International Register (EUROCAT). Chromosomal pathology is identified in 1283
(28,1%) patients, monogenic – in 964 (21,1%) cases. In congenital malformations the largest proportion
belongs to congenital malformations of blood circulation – 694 cases (29,9%), followed by congenital
malformations of gastrointestinal tract – 304 cases (13,1%), multiple malformations – 275 cases (12,8%),
congenital malformations of urinary system – 298 patients (12,8%), congenital malformations of nervous
system – 217 cases (9,4%), congenital malformations of facial structures (187 patients, 8%) and of
musculoskeletal system with 175 cases (7,5%). The most common of the chromosomal pathologies was
registered Down syndrome with 616 cases (48,1%), Edwards syndrome – in 122(9,5%). Turner syndrome
– in 101(7,9%) cases, Kleinfelter’s syndrome – in 66 (5,1%), Patau syndrome – in 30 (2,3%), other gender
chromosome pathologies – 19 (1,5%), structural rearrangements – 329 (25,6%). Monogenic pathology
was detected in 964 (21,1%) cases. Among monogenic pathology, the most common were osteogenesis
imperfecta, chondrodystrophy, adrenogenital syndrome, congenital hypothyroidism, mucopolysaccharidosis,
Prader-Willi syndrome.
Conclusion: The formation of a database of congenital and hereditary pathologies with full coverage of
all fetuses, newborns and children at an older age, a comprehensive examination of families and clarification
of the diagnosis allows to establish the frequency, structure and dynamics of congenital malformations
and hereditary pathology.
2020-01-01T00:00:00ZTHE ROLE OF THE STANDARD CYTOGENETIC STUDY OF BONE MARROW CELLS IN THE DIAGNOSIS OF ACUTE LYMPHOBLASTIC LEUKEMIA IN CHILDREN
http://nur.nu.edu.kz:80/handle/123456789/5190
THE ROLE OF THE STANDARD CYTOGENETIC STUDY OF BONE MARROW CELLS IN THE DIAGNOSIS OF ACUTE LYMPHOBLASTIC LEUKEMIA IN CHILDREN
Zauatbayeva, G.; Tolegen, N.; Bayanova, M.
Introduction: Acute lymphoblastic leukemia is a disease of the hematopoietic system caused by a violation
of certain functions of the bone marrow. The disease is characterized by excessive proliferation of
white blood cells. Acute lymphocytic leukemia is one of the most common nosologies among pediatric
oncology, which occupies 80% of the total number of available forms of leukemia. According to the clinical
protocols (ALL IC-BFM 2009) for oncohematology, the standard cytogenetic study of metaphases in
leukemic cells is one of the most important methods for diagnosing the most significant and common
numerical and structural aberrations.
Methods: The department of clinical and genetic diagnostics is accredited according to the ISO
15189-2012«Medical laboratories - Requirements for quality and competence» in this field of accreditation.
The study involved 152children under the age of 18 with a diagnosis of acute lymphoblastic
leukemia whose linearity was determined by cytological examination of the bone marrow and flow cytometry.
Short-term cultivation of bone marrow cells in a medium with bovine serum and colcemide was
performed using a standard method. Analysis of G-banding with a resolution of 550-bands was carried
out using the Imager Z1(ZEISS) karyotyping system.
Results: During the analysis of the obtained material 30 (20%) patients were found to have such aberrations
as t(6;16)(q13;p13.1), t(8;21)(q21.3;q22), t(4;12)(p14;q13), t(1;11)(p36.1;q23), del(16)(p11), i(17)
(q10), t(9;10)(q34;q22), der(9)(q32) and the presence of marker chromosomes. In the studied bone marrow
cells, 16% of patients had a hypodiploid set of chromosomes (≤44) which gives a poor prognosis,
since the survival rate of patients in this case is only 30%. However, 60% of patients had a hyperdiploid
set (≥47) which according to some clinical data allows these cases to be attributed to a more favorable
prognosis. 80% had a normal diploid set of chromosomes.
Conclusions: Nowadays cytogenetic study of bone marrow cells in the diagnosis of acute lymphoblastic
leukemia is one of the main and mandatory methods of biological characteristics of the disease that allows
identifying chromosomal abnormalities that are of great importance to stratify the risks, treatment
and prognosis of the disease.
2020-01-01T00:00:00ZCOMPARING SYMPTOMS OF KALACHI SLEEPING SYNDROME WITH KNOWN DISEASES AND CONDITIONS TO DETERMINE CLASSIFICATION OF CAUSAL AGENT. INCIDENCE DENSITY SAMPLING CALCULATIONS
http://nur.nu.edu.kz:80/handle/123456789/5189
COMPARING SYMPTOMS OF KALACHI SLEEPING SYNDROME WITH KNOWN DISEASES AND CONDITIONS TO DETERMINE CLASSIFICATION OF CAUSAL AGENT. INCIDENCE DENSITY SAMPLING CALCULATIONS
Zhalmagambetov, B.; Crape, Byron
Introduction. In 2011some of the residents of Kalachi were afflicted with a sudden acute syndrome,
where they would abruptly fall into an immediate abnormal sleep. These episodes of losing consciousness
could happen anywhere and anytime. By the end of 2015, news reports estimated than 1out of
every 4 residents had developed this syndrome. Both children and adults were afflicted. Males and females
were equally affected with no gender preference. The overall purpose of the study is to characterize
signs and symptoms of the sleeping syndrome to confirm or reject the likely classification of causal
agents, based on the various hypotheses. Those include viruses, fungi, bacteria, chemicals, radiation,
mass sociogenic illness, and gases such as carbon monoxide and carbon dioxide intoxications. Second
purpose will be to assess the risks of developing the syndrome 1st,2nd and 3rd times through incidence
density sampling calculations.
Methods: Analyses on comparisons and contrasts with signs and symptoms and frequencies of signs
and symptoms of the Kalachi sleeping syndrome with those produced by various potential causal agents,
as reported in the published scientific literature. The statistical package SPSS was utilized for data management
and statistical applications. Analyses on the risks (overall hazard) of developing disease the first
time versus the second or third time, to determine if the sleeping syndrome conveyed any “immunity”,
utilized a probabilistic theoretical method sometimes utilized in survival analysis related to time to episode,
called incidence density sampling.
Results: Among the leading reported symptoms were spinning head, headache followed by fatique and
memory loss, significantly less number of people reported fever. The probability of getting 1st episode
equals to 0.2014 and 2nd episode equals to 0.3314. There is a statistically significant high chances to get
2nd episode if one already had a previous case (p=0.005). Finally, for the 3rd episode equals to 0.2615.
Conclusion: Symptomatically and in line with the previous household investigation current hypothesis
of incapacitant water transmission seems reasonable. Infectious nature of the cause is less likely due to
absence of immunity after the first exposure as it is evidenced from density sampling calculations.
2020-01-01T00:00:00ZDETERMINATION OF ANTIBACTERIAL SENSITIVITY OF BACTEROIDES FRAGILIS ISOLATED FROM INTRAABDOMINAL INFECTIONS
http://nur.nu.edu.kz:80/handle/123456789/5188
DETERMINATION OF ANTIBACTERIAL SENSITIVITY OF BACTEROIDES FRAGILIS ISOLATED FROM INTRAABDOMINAL INFECTIONS
Kozhakhmetova, S.; Zholdybayeva, E.; Atavliyeva, S.; Tarlykov, P.; Mukhtarova, K.; Syzdykov, T.; Khasenov, R.; Ramankulov, E.
Introduction: Modern intra-abdominal infections are characterized by rapidly growing antibiotic resistance.
Gram-negative bacillus B.fragilis is one of the dominating anaerobic pathogens. Thus this research
aims to study sensitivity of B.fragilis, isolated from patients’ peritoneal exudate , to antibacterial drugs.
Methods: Clinical samples from 72patients from the surgical department of the City Hospital No. 1and
the Multidisciplinary Regional Hospital No. 2(Nur-Sultan) from 2018 to 2019 were collected to form B.fragilis
collection. All isolated bacteroid strains were identified using MALDI-TOF MS (with a score of ≥2) and
via identification of a direct nucleotide sequence of the 16SrRNA gene fragment. Minimum inhibitory
concentration (MIC) of antibiotics was determined using M.I.C. strips (Oxoid, England) with antibiotic
concentration gradients plotted. Four antimicrobial agents were tested: ciprofloxacin, metronidazole,
meropenem and clindamycin.
Results: Overall, 9 strains of anaerobic bacteria B.fragilis were isolated from 72clinical samples of various
intra-abdominal infections. It was found that in intraabdominal infections after B.fragilis extraction,
often B. thetaiotaomicron, Parabacteroides distasonis, B. ovatus, less often B. clarus, Prevotella heparinolytica
and B.salyersiae are extracted. After antibiotic sensitivity test of B. fragilis, it is found that most
of the studied cultures (67%) are ciprofloxacin resistant and smaller part (14%) is resistant to metronidazole.
25% of the strains showed moderate sensitivity to meropenem, 33% to ciprofloxacin, 50% to clindamycin
and 57% to metronidazole. 29% of the strains demonstrated high sensitivity to metronidazole and
50% to clindamycin. The highest sensitivity of B. fragilis (75%) was to meropenem (carbapenem) among
all antimicrobial agents tested.
Conclusions: Thus, obtained results help to elucidate inclusion of drugs that are the most active against
the B.fragilis. Namely, inclusion of meropenem as monotherapy and metronidazole in combination with
other antibiotics for the treatment of intra-abdominal infections.
2020-01-01T00:00:00ZETIOLOGICAL STRUCTURE OF ACUTE BRONCHIOLITIS IN YOUNG CHILDREN
http://nur.nu.edu.kz:80/handle/123456789/5187
ETIOLOGICAL STRUCTURE OF ACUTE BRONCHIOLITIS IN YOUNG CHILDREN
Turdalina, B.R.; Bayesheva, D.A.; Seydullaeva, A.Zh.; Omarova, A.K.; Esimkhanova, G.O.; Zhumabek, P.
Introduction: Bronchiolitis is most common in children under the age of 9 months in (90% of cases).
Every year, 150 million cases of bronchiolitis are recorded in the world, 7-13% of which require inpatient
treatment and 1-3% of hospitalization in the intensive care unit. We conducted a prospective study to
analyze the etiological structure of acute bronchiolitis in young children.
Methods: medical histories of 50 patients treated in the viral department with suspected acute bronchiolitis
from the age of 1month to 1year for the period between September 2019 to December 2019 were
analyzed. To determine the etiological structure, a PCR study of ARVI-screen FL was used on the basis of
the clinical laboratory of Multidisciplinary City Children’s Hospital No. 3.
Results: From 50 analyzed medical histories with the clinical criterion of acute bronchiolitis, but with a
preliminary diagnosis of obstructive bronchitis, the subsequent diagnosis was confirmed as acute bronchiolitis
in 42% of patients, 46% of obstructive bronchitis and 12% were confirmed as community-acquired
pneumonia. 60% Of the analyzed patients were paratrophic by type of physique, which may serve
as a factor affecting the preservation of prolonged bronchial obstructive syndrome in young children.
The presence of shortness of breath is the main clinical symptom that determines the severity of the
disease. In all analyzed patients bronchial obstructive syndrome (BOS) persisted for three days and only
on the 4th day of hospitalization BOS was stopped in 84% of patients. In the remaining 16% of patients
this syndrome persisted specifically in the second group of patients who were given a clinical diagnosis:
Acute bronchiolitis. Using a PCR study of ARVI-screen FL, it was found that in the group of children with
a clinical diagnosis of “Acute bronchiolitis”, in 64% of patients respiratory syncytial virus was found, and
the virus was not isolated in 14% of patients, on third place was bocavirus (8 %).
Conclusions: there is no preliminary diagnosis at the receiving ward level: Acute bronchiolitis. In the
etiological structure of acute bronchiolitis the respiratory syncytial virus was dominant, which was confirmed
in 64% of patients using PCR studies.
2020-01-01T00:00:00Z