Research Institute of Bioengineering, Regenerative Medicine and Neurophysiologyhttp://nur.nu.edu.kz:80/handle/123456789/11252024-03-28T19:34:34Z2024-03-28T19:34:34ZMESENCHYMAL STEM CELLS COATED WITH SYNTHETIC BONE-TARGETING POLYMERS ENHANCE OSTEOPOROTIC BONE FRACTURE REGENERATIONSafarova, YuliyaOlzhayev, FarkhadUmbayev, BauyrzhanTsoy, AndreyHortelano, GonzaloTokay, TursonjanMurata, HironobuRussell, AlanAskarova, Sholpanhttp://nur.nu.edu.kz:80/handle/123456789/60182022-02-04T06:35:38Z2020-10-12T00:00:00ZMESENCHYMAL STEM CELLS COATED WITH SYNTHETIC BONE-TARGETING POLYMERS ENHANCE OSTEOPOROTIC BONE FRACTURE REGENERATION
Safarova, Yuliya; Olzhayev, Farkhad; Umbayev, Bauyrzhan; Tsoy, Andrey; Hortelano, Gonzalo; Tokay, Tursonjan; Murata, Hironobu; Russell, Alan; Askarova, Sholpan
Osteoporosis is a progressive skeletal disease characterized by reduced bone density leading to bone fragility and an elevated risk of bone fractures. In osteoporotic conditions, decrease in bone density happens due to the augmented osteoclastic activity and the reduced number of osteoblast progenitor cells (mesenchymal stem cells, MSCs). We investigated a new method of cell therapy with membrane-engineered MSCs to restore the osteoblast progenitor pool and to inhibit osteoclastic activity in the fractured osteoporotic bones. The primary active sites of the polymer are the N-hydroxysuccinimide and bisphosphonate groups that allow the polymer to covalently bind to the MSCs’ plasma membrane, target hydroxyapatite molecules on the bone surface and inhibit osteolysis. The therapeutic utility of the membrane-engineered MSCs was investigated in female rats with induced estrogen-dependent osteoporosis and ulnar fractures. The analysis of the bone density dynamics showed a 27.4% and 21.5% increase in bone density at 4 and 24 weeks after the osteotomy of the ulna in animals that received four transplantations of polymer-modified MSCs. The results of the intravital observations were confirmed by the post-mortem analysis of histological slices of the fracture zones. Therefore, this combined approach that involves polymer and cell transplantation shows promise and warrants further bio-safety and clinical exploration.
2020-10-12T00:00:00ZGALACTOSE-INDUCED SKIN AGING: THE ROLE OF OXIDATIVE STRESSUmbayev, BauyrzhanAskarova, SholpanAlmabayeva, AigulSaliev, TimurMasoud, Abdul-RazakBulanin, Denishttp://nur.nu.edu.kz:80/handle/123456789/55312021-07-08T21:00:31Z2020-06-17T00:00:00ZGALACTOSE-INDUCED SKIN AGING: THE ROLE OF OXIDATIVE STRESS
Umbayev, Bauyrzhan; Askarova, Sholpan; Almabayeva, Aigul; Saliev, Timur; Masoud, Abdul-Razak; Bulanin, Denis
Skin aging has been associated with a higher dietary intake of carbohydrates, particularly glucose and galactose. In fact, the carbohydrates are capable of damaging the skin’s vital components through nonenzymatic glycation, the covalent attachment of sugar to a protein, and subsequent production of advanced glycation end products (AGEs). This review is focused on the role of D-galactose in the development of skin aging and its relation to oxidative stress. The interest in this problem was dictated by recent findings that used in vitro and in vivo models. The review highlights the recent advances in the underlying molecular mechanisms of D-galactose-mediated cell senescence and cytotoxicity. We have also proposed the possible impact of galactosemia on skin aging and its clinical relevance. The understanding of molecular mechanisms of skin aging mediated by D-galactose can help dermatologists optimize methods for prevention and treatment of skin senescence and aging-related skin diseases.
2020-06-17T00:00:00ZIMPROVEMENT OF NEUROLOGICAL FUNCTION IN RATS WITH ISCHEMIC STROKE BY ADIPOSEDERIVED PERICYTESOgay, VyacheslavKumasheva, VeneraLi, YelenaMukhlis, SholpanSekenova, AliyaOlzhayev, FarkhadTsoy, AndreyUmbayev, BaurzhanAskarova, SholpanShpekov, AzatKaliyev, AssylbekZhetpisbayev, BerikMakhambetov, YerbolAkshulakov, SerikSaparov, ArmanRamankulov, Yerlanhttp://nur.nu.edu.kz:80/handle/123456789/55172021-07-02T21:00:39Z2020-09-04T00:00:00ZIMPROVEMENT OF NEUROLOGICAL FUNCTION IN RATS WITH ISCHEMIC STROKE BY ADIPOSEDERIVED PERICYTES
Ogay, Vyacheslav; Kumasheva, Venera; Li, Yelena; Mukhlis, Sholpan; Sekenova, Aliya; Olzhayev, Farkhad; Tsoy, Andrey; Umbayev, Baurzhan; Askarova, Sholpan; Shpekov, Azat; Kaliyev, Assylbek; Zhetpisbayev, Berik; Makhambetov, Yerbol; Akshulakov, Serik; Saparov, Arman; Ramankulov, Yerlan
Pericytes possess high multipotent features and cell plasticity, and produce angiogenic and neurotrophic factors that indicate their
high regenerative potential. The aim of this study was to investigate whether transplantation of adipose-derived pericytes can
improve functional recovery and neurovascular plasticity after ischemic stroke in rats. Rat adipose-derived pericytes were isolated from subcutaneous adipose tissue by fluorescence-activated cell sorting. Adult male Wistar rats were subjected to 90 min of
middle cerebral artery occlusion followed by intravenous injection of rat adipose-derived pericytes 24 h later. Functional recovery
evaluations were performed at 1, 7, 14, and 28 days after injection of rat adipose-derived pericytes. Angiogenesis and neurogenesis were examined in rat brains using immunohistochemistry. It was observed that intravenous injection of adipose-derived
pericytes significantly improved recovery of neurological function in rats with stroke compared to phosphate-buffered saline–
treated controls. Immunohistochemical analysis revealed that the number of blood capillaries was significantly increased along the
ischemic boundary zone of the cortex and striatum in stroke rats treated with adipose-derived pericytes. In addition, treatment
with adipose-derived pericytes increased the number of doublecortin positive neuroblasts. Our data suggest that transplantation
of adipose-derived pericytes can significantly improve the neurologic status and contribute to neurovascular remodeling in rats
after ischemic stroke. These data provide a new insight for future cell therapies that aim to treat ischemic stroke patients.
2020-09-04T00:00:00ZMesenchymal stem cells modifications for enhanced bone targeting and bone regenerationSafarova, YuliyaUmbayev, BauyrzhanHortelano, GonzaloAskarova, Sholpanhttp://nur.nu.edu.kz:80/handle/123456789/49242020-08-21T21:00:19Z2020-04-16T00:00:00ZMesenchymal stem cells modifications for enhanced bone targeting and bone regeneration
Safarova, Yuliya; Umbayev, Bauyrzhan; Hortelano, Gonzalo; Askarova, Sholpan
In pathological bone conditions (e.g., osteoporotic fractures or critical size bone defects), increasing the pool of osteoblast progenitor cells is a promising therapeutic approach to facilitate bone healing. Since mesenchymal stem cells (MSCs) give rise to the osteogenic lineage, a number of clinical trials investigated the potential of MSCs transplantation for bone regeneration. However, the engraftment of transplanted cells is often hindered by insufficient oxygen and nutrients supply and the tendency of MSCs to home to different sites of the body. In this review, we discuss various approaches of MSCs transplantation for bone regeneration including scaffold and hydrogel constructs, genetic modifications and surface engineering of the cell membrane aimed to improve homing and increase cell viability, proliferation and differentiation.
2020-04-16T00:00:00ZИЗУЧЕНИЕ ФЕНОЛЬНЫХ СОЕДИНЕНИЙ ЯГОД ТРЕХ ВИДОВ РАСТЕНИЙ РОДА VACCINIUM, ПРОИЗРАСТАЮЩИХ В ХАНТЫМАНСИЙСКОМ АВТОНОМНОМ ОКРУГЕGulyayev, AlexandrГуляев, АлександрБелова, ЕкатеринаТритэк, ВладиславШульгау, ЗаринаКривых, ЕленаКоваленко, ЛюдмилаДренин, АлексейБотиров, Эркинhttp://nur.nu.edu.kz:80/handle/123456789/46462020-05-12T21:00:39Z2020-03-05T00:00:00ZИЗУЧЕНИЕ ФЕНОЛЬНЫХ СОЕДИНЕНИЙ ЯГОД ТРЕХ ВИДОВ РАСТЕНИЙ РОДА VACCINIUM, ПРОИЗРАСТАЮЩИХ В ХАНТЫМАНСИЙСКОМ АВТОНОМНОМ ОКРУГЕ
Gulyayev, Alexandr; Гуляев, Александр; Белова, Екатерина; Тритэк, Владислав; Шульгау, Зарина; Кривых, Елена; Коваленко, Людмила; Дренин, Алексей; Ботиров, Эркин
Впервые проведено исследование химического состава полифенольных соединений плодов клюквы (Vaccinium oxycoccus L.), черники (Vaccinium myrtillus L.) и брусники (Vaccinium vitis-idaea L.), произрастающих на территории Сургутского района Ханты-Мансийского автономного округа – Югры. Свежие ягоды пятикратно экстрагировали 80%-ной водно-спиртовой смесью при комнатной температуре. Полученные экстракты объединяли, фильтровали, растворитель из фильтрата отгоняли под вакуумом на ротационном испарителе. В составе полученных экстрактов методом ВЭЖХ идентифицировано 15 гликозидов антоцианидинов, а также 30 фенольных соединений, относящихся к гидроксибензойным и гидроксикоричным кислотам, флавонолам, катехинам и процианидинам. Идентификация фенольных соединений проводилась в соответствии с временами удерживания и спектрами поглощения соответствующих аналитических стандартов. Основным фенольным соединением экстракта ягод клюквы является хлорогеновая кислота. Установлен рост содержания полифенолов в ряду: клюква–брусника–черника. Полученные данные в целом по содержанию полифенолов сопоставимы с результатами исследования клюквы и черники из региона Аляски, несущественно отличаясь по содержанию антоцианов и отдельных флавонов. Значительные отличия по фенольным компонентам выявляются при сравнении наших данных с литературными данными по бруснике и клюкве из районов севера и востока Европы (Польша, Белоруссия, Норвегия). Обнаруженные количественные различия по отдельным полифенолам, вероятно, связаны с географическими и местными природными условиями. Высокое содержание полифенолов в изученных нами ягодах позволяет предполагать возможность использования их в качестве фармацевтического сырья.
2020-03-05T00:00:00ZThe Links Between the Gut Microbiome, Aging, Modern Lifestyle and Alzheimer's DiseaseAskarova, SholpanUmbayev, BauyrzhanMasoud, Abdul-RazakKaiyrlykyzy, AiymSafarova, YuliyaTsoy, AndreyOlzhayev, FarkhadKushugulova, Almagul R.http://nur.nu.edu.kz:80/handle/123456789/46042020-05-06T21:00:30Z2020-03-18T00:00:00ZThe Links Between the Gut Microbiome, Aging, Modern Lifestyle and Alzheimer's Disease
Askarova, Sholpan; Umbayev, Bauyrzhan; Masoud, Abdul-Razak; Kaiyrlykyzy, Aiym; Safarova, Yuliya; Tsoy, Andrey; Olzhayev, Farkhad; Kushugulova, Almagul R.
Gut microbiome is a community of microorganisms in the gastrointestinal tract. These bacteria have a tremendous impact on the human physiology in healthy individuals and during an illness. Intestinal microbiome can influence one's health either directly by secreting biologically active substances such as vitamins, essential amino acids, lipids et cetera or indirectly by modulating metabolic processes and the immune system. In recent years considerable information has been accumulated on the relationship between gut microbiome and brain functions. Moreover, significant quantitative and qualitative changes of gut microbiome have been reported in patients with Alzheimer's disease. On the other hand, gut microbiome is highly sensitive to negative external lifestyle aspects, such as diet, sleep deprivation, circadian rhythm disturbance, chronic noise, and sedentary behavior, which are also considered as important risk factors for the development of sporadic Alzheimer's disease. In this regard, this review is focused on analyzing the links between gut microbiome, modern lifestyle, aging, and Alzheimer's disease.
2020-03-18T00:00:00Z