2024-03-28T16:49:06Zhttp://nur.nu.edu.kz/oai/requestoai:nur.nu.edu.kz:123456789/35152021-02-05T07:15:16Zcom_123456789_434com_123456789_70com_123456789_67col_123456789_514
2018-09-17T04:30:02Z
urn:hdl:123456789/3515
Application of advanced molecular techniques for analyzing phytoplankton population in Burabay National Nature Park Resort
Sarsembekova, Assel
phytoplankton
Burabay National Nature Park Resort
phytoplankton population
In this work, the molecular techniques as DNA extraction, DNA quantification, PCR analysis were optimized and subsequent sequencing was performed on MiSeq platform (Illumina, USA). We aimed to apply molecular methods to analyse phytoplankton population inhabiting Lake Shortan and Lake Burabay
2018-09-17T04:30:02Z
2018-09-17T04:30:02Z
2016
Master's thesis
Assel Sarsembekova. 2016. Application of advanced molecular techniques for analyzing phytoplankton population in Burabay National Nature Park Resort. Nazarbayev University. School of Science and Technology
http://nur.nu.edu.kz/handle/123456789/3515
Nazarbayev University School of Science and Technology
oai:nur.nu.edu.kz:123456789/35162021-02-05T07:15:19Zcom_123456789_434com_123456789_70com_123456789_67col_123456789_514
2018-09-17T05:02:34Z
urn:hdl:123456789/3516
Concentration dependent mitostatic effect and inhibition of cell motibility by different tubulin binding drugs-comparative study
Balabiyev, Arnat
nocodazole
taxol
vinorelbine
mitotic progression
cell motility
Here, we demonstrate the comparative effects of nocodazole, taxol and vinorelbine on mitotic progression and cell motility using mouse fibroblasts and three cancer cell lines
2018-09-17T05:02:34Z
2018-09-17T05:02:34Z
2016
Master's thesis
Arnat Balabiyev. Concentration dependent mitostatic effect and inhibition of cell motibility by different tubulin binding drugs-comparative study. 2016. Nazarbayev University. School of Science and Technology
http://nur.nu.edu.kz/handle/123456789/3516
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Nazarbayev University School of Science and Technology
oai:nur.nu.edu.kz:123456789/35172021-02-05T07:15:22Zcom_123456789_434com_123456789_70com_123456789_67col_123456789_514
2018-09-17T05:16:36Z
urn:hdl:123456789/3517
Morphometric characteristics of cancer cells grown in embryonic microenvironment and under antiviral treatment
Shaimerdenova, Madina
cancer cell lines
cancer
embryonic microenvironment treatment
antiviral treatment
We describe here that both embryonic microenvironment treatment and antiviral treatment have the potential to serve as effective factors in changing morphometric characteristics of two highly aggressive cancer cell lines, with proven capability to decrease viability of cancer cells, reduce amount of colonies formed after treatment, alter intracellular features of cell such as nucleus and cytoskeleton and diminish aldehyde dehydrogenase activity after treatment
2018-09-17T05:16:36Z
2018-09-17T05:16:36Z
2016
Master's thesis
Madina Shaimerdenova. Morphometric characteristics of cancer cells grown in embryonic microenvironment and under antiviral treatment. 2016. Nazarbayev University. School of Science and Technology
http://nur.nu.edu.kz/handle/123456789/3517
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Nazarbayev University School of Science and Technology
oai:nur.nu.edu.kz:123456789/35192021-02-05T07:15:26Zcom_123456789_434com_123456789_70com_123456789_67col_123456789_514
2018-09-17T05:51:11Z
urn:hdl:123456789/3519
Co-expression of FVIII with human IGG FC Fragment or mouse IL-10 in encapsulated G8 myoblast cells as a potential treatment of hemophilia A
Kanketayeva, Zhansaya
FVIII
G8
IL-10
Fc
In this master thesis study we have shown that recombination G8 myoblasts, encapsulated in alginate-PLL microcapsules can successfully secrete detectable levels of both Fc and IL-10 out of the capsules and sustain good viability, showing the potential of co-delivering FVIII by encapsulated cells.
2018-09-17T05:51:11Z
2018-09-17T05:51:11Z
2016
Master's thesis
Zhansaya Kanketayeva. Co-expression of FVIII with human IGG FC Fragment or mouse IL-10 in encapsulated G8 myoblast cells as a potential treatment of hemophilia A. 2016. Nazarbayev University. School of Science and Technology
http://nur.nu.edu.kz/handle/123456789/3519
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Nazarbayev University School of Science and Technology
oai:nur.nu.edu.kz:123456789/35182021-02-05T07:15:28Zcom_123456789_434com_123456789_70com_123456789_67col_123456789_514
2018-09-17T05:50:56Z
urn:hdl:123456789/3518
Role of astrocyte aging in the pathogenesis of Alzheimer`s disease
Imangali, Nurgul
Alzheimer`s disease
In this study we presented in vitro model for replicative senescence of primary human astrocytes isolated from fetal brains
2018-09-17T05:50:56Z
2018-09-17T05:50:56Z
2016
Master's thesis
Nurgul Imangali. Role of astrocyte aging in the pathogenesis of Alzheimer`s disease. 2016. Nazarbayev University. School of Science and Technology
http://nur.nu.edu.kz/handle/123456789/3518
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Nazarbayev University School of Science and Technology
oai:nur.nu.edu.kz:123456789/35202021-02-05T07:15:31Zcom_123456789_434com_123456789_70com_123456789_67col_123456789_514
2018-09-17T06:08:01Z
urn:hdl:123456789/3520
Development of flow cytometry and microscopy techniques to monitor freshwater phytoplankton communities in Kazakhstani lakes
Dashkova, Veronika
freshwater phytoplankton communities
In this project, we tested an approach based on separating fluorescent viability and metabolic dyes between different excitation lasers in order to reach minimal spectral overlap with the autofluorescent signal using flow and imaging cytometry
2018-09-17T06:08:01Z
2018-09-17T06:08:01Z
2016
Master's thesis
Veronika Dashkova. Development of flow cytometry and microscopy techniques to monitor freshwater phytoplankton communities in Kazakhstani lakes. 2016. Nazarbayev University. School of Science and Technology
http://nur.nu.edu.kz/handle/123456789/3520
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Nazarbayev University School of Science and Technology
oai:nur.nu.edu.kz:123456789/35212021-02-05T07:15:34Zcom_123456789_434com_123456789_70com_123456789_67col_123456789_514
2018-09-17T08:55:08Z
urn:hdl:123456789/3521
Migration characteristics of cancer cells grown in embryonic microenvironment and under antiviral agents
Karapina, Orynbassar
cancer
cancer cells
embryonic microenvironment
This study describes a method for investigating reprogramming of cancer cells using chicken embryo extract and dimishing cancer cell progressing with acyclovir and it was used as innovative approach to address whether aggressive cancer cells with high potential to proliferate and migrate to distant organs could respond to the treatments, which could control cell fate determination
2018-09-17T08:55:08Z
2018-09-17T08:55:08Z
2016
Master's thesis
Orynbassar Karapina. Migration characteristics of cancer cells grown in embryonic microenvironment and under antiviral agents. 2016. Nazarbayev University. School of Science and Technology
http://nur.nu.edu.kz/handle/123456789/3521
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Nazarbayev University School of Science and Technology
oai:nur.nu.edu.kz:123456789/35232021-02-05T07:15:36Zcom_123456789_434com_123456789_70com_123456789_67col_123456789_514
2018-09-21T09:44:26Z
urn:hdl:123456789/3523
Oral administration of chitosan/DNA nanoparticles containing DNA coding for FVIII and FC IgG fragment or IL-10 for the modulation of immune responses to FVIII in hemophilia mice
Yerkesh, Zhadyra
Hemophilia A
FVIII
DNA
Hemophilia A is an X-linked bleeding disorder, which occurs due to deficiency of
clotting protein FVIII. Current treatment involves regular lifelong infusion of
recombinant or plasma-derived FVIII protein, which is suboptimal, invasive and very
expensive. One of the biggest challenges of the current therapy is the recognition of
FVIII by immune system as a foreign substance, leading to the development of
neutralizing antibodies, which makes further administration of FVIII ineffective.
Therefore, an alternative cost effective and safe treatment to Hemophilia A is highly
desirable. We propose chitosan-mediated oral non-viral gene therapy as a safe and costeffective
strategy for immune modulation in severe hemophilia A cases. Having a
strong affinity for DNA and forming nanoparticles, chitosan-DNA complexes protect
plasmid DNA fi-om degradation by the low pH envu-onment of the stomach and from
nucleases in the GI tract; further, safe re-administration of nanoparticles is possible. We
also propose to include in the plasmid DNA coding for the Fc fragment of IgG heavy
chain region, which contains Tregitope sequences, T cell epitopes that specifically
activate regulatory T cells or anti-inflammatory cytokine IL-10 that may modulate the
immune response against FVIII protein. Therefore, it is hypothesized that orally
administered chitosan/DNA nanoparticles will lead to clinically relevant amount of
FVIII in the host without an immune response, providing long term, cost-effective and
safe treatment for hemophilia A.
For this study we aimed to: I) optimize nanoparticles in terms of effective gene
expression in vitro; 2) treat hemophilic mice orally with chitosan nanoparticles
containing DNA coding for FVIII and for immunomodulatory elements (Fc fragment of
IgG and IL-10) to induce tolerance to FVIII. We found that the key factors contributing
to the effectiveness of gene expression in chitosan/DNA nanoparticles are the type of
chitosan, the charge ratio (N/P) and the DNA concentration. In vivo, the optimized
nanoparticles containing FVIII+Fc DNA significantly decreased antibody formation
specific to F V I I I in prophylactic group of mice, and in 4/6 mice receiving nanoparticles
containing FVIII+IL-10. It is tempting to hypothesize that this strategy might also be
extended to modulate immune responses to other antigens.
2018-09-21T09:44:26Z
2018-09-21T09:44:26Z
2016
Master's thesis
Zhadyra Yerkesh. 2016. Oral administration of chitosan/DNA nanoparticles containing DNA coding for FVIII and FC IgG fragment or IL-10 for the modulation of immune responses to FVIII in hemophilia mice. Nazarbayev University. School of Science and Technology
http://nur.nu.edu.kz/handle/123456789/3523
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Nazarbayev University School of Science and Technology