2024-03-28T18:07:17Zhttp://nur.nu.edu.kz/oai/requestoai:nur.nu.edu.kz:123456789/9822018-08-15T03:49:50Zcom_123456789_104com_123456789_67col_123456789_105
Bacterial growth in media mimicking the high salt and alkalinity of extreme kazakhstan environments results in production of antimicrobial compounds in soil actinomycetes isolated from these extremophile locations
Roberts, Jill
Trenozhnikova, Lyudmila
Seaton, Lylah
Patel, Ami
Faza, Colton
Whitaker, Jacqueline
Mayer, Christen
Azizan, Azliyati
Streptomyces
Extremophiles
antagonists
antibacterial
antibiotics
HA-MRSA
Research Subject Categories::MEDICINE
Increasing antibiotic resistance among multidrug resistant pathogens necessitates the search for newer antimicrobials. Streptomyces historically produce the largest number of antibacterials and herein we describe isolation of antagonists from extremophiles using unusual culture media. Antagonists or antimicrobials produced under extremophile environmental conditions demonstrated activity against MRSA from Kazakhstan and the United States.
2015-12-30T04:09:15Z
2015-12-30T04:09:15Z
2013-04-13
Article
2050-120X
http://nur.nu.edu.kz/handle/123456789/982
en
Journal of Pharmaceutical Technology & Drug Research
oai:nur.nu.edu.kz:123456789/9832018-08-15T03:49:41Zcom_123456789_104com_123456789_67col_123456789_105
Lack of Clinical Manifestations in Asymptomatic Dengue Infection Is Attributed to Broad Down-Regulation and Selective Up-Regulation of Host Defence Response Genes
Yeo, Adeline
Azhar, Nur Atiqah
Yeow, Wanyi
Talbot Jr., C. Conover
Asif Khan, Mohammad
Shankar, Esaki M.
Rathakrishnan, Anusyah
Azizan, Azliyati
Wang, Seok Mui
Kim Lee, Siew
Yik Fong, Mun Yik
Manikam, Rishya
Devi Sekaran, Shamala
clinical manifestations
asymptomatic dengue
gene expression
Dengue represents one of the most serious life-threatening vector-borne infectious diseases that afflicts approximately 50 million people across the globe annually. Whilst symptomatic infections are frequently reported, asymptomatic dengue remains largely unnoticed. Therefore, we sought to investigate the immune correlates conferring protection to individuals that remain clinically asymptomatic.
2015-12-30T04:55:45Z
2015-12-30T04:55:45Z
2014-04
Article
Yeo ASL, Azhar NA, Yeow W, Talbot CC Jr, Khan MA, Shankar EM, et al. (2014) Lack of Clinical Manifestations in Asymptomatic Dengue Infection Is Attributed to Broad Down-Regulation and Selective Up-Regulation of Host Defence Response Genes. PLoS ONE 9(4): e92240. doi:10.1371/journal.pone.0092240
http://nur.nu.edu.kz/handle/123456789/983
en
PLOS ONE:Open Access journal
oai:nur.nu.edu.kz:123456789/9842018-08-15T03:49:46Zcom_123456789_104com_123456789_67col_123456789_105
Clinical and Immunological Markers of Dengue Progression in a Study Cohort from a Hyperendemic Area in Malaysia
Rathakrishnan, Anusyah
Klekamp, Benjamin
Seok, Mui Wang
Komarasamy, Thamil Vaani
Natkunam, Santha Kumari
Sathar, Jameela
Azizan, Azliyati
Sanchez-Anguiano, Aurora
Manikam, Rishya
Devi Sekaran, Shamala
Malaysia
immunological markers
dengue
Cohort
Background: With its elusive pathogenesis, dengue imposes serious healthcare, economic and social burden on endemic countries. This study describes the clinical and immunological parameters of a dengue cohort in a Malaysian city, the first
according to the WHO 2009 dengue classification. Methodology and Findings: This longitudinal descriptive study was conducted in two Malaysian hospitals where patients
aged 14 and above with clinical symptoms suggestive of dengue were recruited with informed consent. Among the 504 participants, 9.3% were classified as non-dengue, 12.7% without warning signs, 77.0% with warning signs and 1.0% with
severe dengue based on clinical diagnosis. Of these, 37% were misdiagnosed as non-dengue, highlighting the importance
of both clinical diagnosis and laboratory findings. Thrombocytopenia, prolonged clotting time, liver enzymes, ALT and AST served as good markers for dengue progression but could not distinguish between patients with and without warning signs. HLA-A*24 and -B*57 were positively associated with Chinese and Indians patients with warning signs, respectively, whereas A*03 may be protective in the Malays. HLA-A*33 was also positively associated in patients with warning signs when compared to those without. Dengue NS1, NS2A, NS4A and NS4B were found to be important T cell epitopes; however with no apparent difference between with and without warning signs patients. Distinction between the 2 groups of patients was also not observed in any of the cytokines analyzed; nevertheless, 12 were significantly differentially expressed at the
different phases of illness.
2015-12-30T05:04:49Z
2015-12-30T05:04:49Z
2014-03
Article
Rathakrishnan A, Klekamp B, Wang SM, Komarasamy TV, Natkunam SK, et al. (2014) Clinical and Immunological Markers of Dengue Progression in a Study Cohort from a Hyperendemic Area in Malaysia. PLoS ONE 9(3): e92021. doi:10.1371/journal.pone.0092021
http://nur.nu.edu.kz/handle/123456789/984
en
Clinical Epidemiology of a Malaysian Dengue Cohort
oai:nur.nu.edu.kz:123456789/9852018-08-15T03:49:48Zcom_123456789_104com_123456789_67col_123456789_105
Assessment of Risk Factors of Helicobacter Pylori Infection and Peptic Ulcer Disease
Mhaskar, Rahul S.
Ricardo, Izurieta
Azizan, Azliyati
Laxminarayan, Rajaram
Amol, Bapaye
Santosh, Walujkar
Boo, Kwa
Case control study
Chili peppers
Helicobacter pylori
India
Parasites
Pune
Background: Helicobacter pylori (H. pylori) infection is a risk factor for peptic ulcer. There have been no studies addressing environmental and dietary risk factors in western India. We conducted a case control study enrolling peptic ulcer patients in Pune, India. Materials and Methods: Risk factors for peptic ulcer and H. pylori infection were assessed in a participant interview. H. pylori status was assessed from stool by monoclonal antigen detection. Results: We enrolled 190 peptic ulcer, 35 stomach cancer patients, and 125 controls. Fifty-one percent (180/350) of the participants were infected with H. pylori. Lower socioeconomic status (SES) [odds ratio (OR): 1.10, 95% confidence interval (CI): 1.02-1.39], meat consumption (OR: 2.35, 95% CI: 1.30-4.23), smoking (OR: 2.23, 95% CI: 1.24-4.02), eating restaurant food (OR: 3.77, 95% CI: 1.39-10.23), and drinking nonfiltered or nonboiled water (OR: 1.05, 95% CI: 1.01-1.23) were risk factors for H. pylori infection. H. pylori infection (OR: 1.70, 95% CI:I. 03-2.89), meat (OR: 1.10, 95% CI: 1.02-1.75), fish (OR: 1.05, 95% CI: 1.02-1.89) consumption, and a family history of ulcer
(OR: 1.20, 95% CI: 1.08-1.60) were risk factors for peptic ulcer. Consumption of chili peppers (OR: 0.20, 95% CI: 0.10-0.37) and parasite infestation (OR: 0.44, 95% CI: 0.24-0.80) were protective against H. pylori infection. Conclusion: H. pylori infection is
associated with peptic ulcer. Lower SES, consumption of restaurant food, meat, nonfiltered water, and smoking are risk factors for H. pylori. Consumption of meat, fish, and a family history of peptic ulcer are risk factors for peptic ulcer. Consumption of chili
peppers and concurrent parasite infestation appear to be protective against H. pylori.
2015-12-30T05:19:21Z
2015-12-30T05:19:21Z
2013
Article
http://nur.nu.edu.kz/handle/123456789/985
en
Journal of Global Infectious Diseases
oai:nur.nu.edu.kz:123456789/9872018-08-15T03:49:50Zcom_123456789_104com_123456789_67col_123456789_105
New recreational water testing alternatives
Kesteloot, Kurt
Azizan, Azliyati
Whitman, Richard
Nevers, Meredith
advanced notification
empirical predictive modeling
qPCR
rainfall threshold levels
recreational water testing
water-quality testing
Research Subject Categories::MEDICINE
Each year recreational water users descend on national parks by the
millions. U.S. Environmental Protection Agency (EPA) regulations require monitoring
waters for fecal indicator bacteria in order to safeguard human health, and obtaining
results using the culturing method takes 18 hours or more of analytical time. Thus,
under this surveillance regime swimmers can be exposed to waterborne disease
organisms before health advisories can be issued. To address the need for timelier
notification of recreational water quality, the EPA has evaluated and approved new and
faster testing methods as of November 2012. This article discusses new recreational
water testing methodologies such as qPCR, empirical predictive modeling, rainfall
threshold levels, and advanced notification options for park managers to consider and
tailor to their needs.
2015-12-30T05:29:04Z
2015-12-30T05:29:04Z
2013-03-13
Article
http://nur.nu.edu.kz/handle/123456789/987
en
Park Science is a research and resource management journal
oai:nur.nu.edu.kz:123456789/10752018-08-15T03:49:48Zcom_123456789_104com_123456789_67col_123456789_105
Current global status of dengue diagnostics
D. Smith, Miranda
Azizan, Azliyati
dengue
diagnostics
commercial
rapid
point-of-care
tests
Dengue Virus is a re-emerging infectious disease that is transmitted through mosquitos.
Dengue is a significant health concern because of the number of people it affects globally. Clinical diagnosis of dengue is not possible because the symptoms are similar to other febrile-diseases. Therefore, the only way to truly diagnose dengue is via laboratory methods. Many diagnostics tests exist to accomplish this; however, these tests have disadvantages. Rapid, point-of-care, commercially available diagnostic test kits have come onto the market to bridge the gap for those without high tech laboratories and personnel. This paper extends the knowledge of a meta-analysis conducted in 2011 on the performance of commercially available diagnostic tests. The purpose of
this review was to compare and contrast the accuracy of commercial dengue diagnostic tests.
2016-01-28T11:21:31Z
2016-01-28T11:21:31Z
2014-12-14
Article
http://nur.nu.edu.kz/handle/123456789/1075
en
Journal of Advances in Biology & Biotechonology
oai:nur.nu.edu.kz:123456789/10762018-08-15T03:49:50Zcom_123456789_104com_123456789_67col_123456789_105
Dengue and mosquito control programs: a comparative analysis
Falope, Oluyemisi
Hanson, Katheryn
Azizan, Azliyati
dengue
aedes
mosquito control programs
dengue epidemics
Research Subject Categories::MEDICINE
Aims: This paper aims to compare the effectiveness of current mosquito control programs in the control of dengue in a developed versus a developing country.
Study Design: Systematic Review and Analysis. Place and Duration of Study: Department of Global Health, University of South Florida, Tampa, Florida, USA between August 2013 and July 2014. Methodology: Systematic literature search of published and grey literature was done using the following databases: MEDLINE, AGRICOLA, BMC, DOAJ, Web of Science, the Cochrane Library, WHOLIS and Google Scholar. Relevant data were then extracted and analyzed. Results: Developing countries usually start up vector control activities after an epidemic has started, resulting in a lower impact on the control and prevention of dengue. Some developing countries, despite having sustainable vector control programs, still have a high incidence of dengue. Some studies have shown that factors such as defective urban planning, low socioeconomic status and poor physical housing conditions in some endemic locations within the developing countries may account for the high incidence of dengue cases.
2016-01-28T11:28:37Z
2016-01-28T11:28:37Z
2014-12-17
Article
http://nur.nu.edu.kz/handle/123456789/1076
en
Journal of Applied Life Sciences International
oai:nur.nu.edu.kz:123456789/13502018-08-15T03:50:25Zcom_123456789_104com_123456789_67col_123456789_105
Peripheral nervous system defects in erbB2 mutants following genetic rescue of heart development
Woldeyesus, Masresha T.
Britsch, Stefan
Riethmacher, Dieter
Xu, Lan
Sonnenberg-Riethmacher, Eva
Abou-Rebyeh, Faikah
Harvey, Richard
Caroni, Pico
Birchmeier, Carmen
Motoneuron loss
neuromuscular synapse
coreceptor
neuregulin
AChR
The ErbB2 tyrosine kinase functions as coreceptor for the neuregulin receptors ErbB3 and ErbB4 and can
participate in signaling of EGF receptor (ErbB1), interleukin receptor gp130, and G-protein coupled receptors.
ErbB2−/− mice die at midgestation because of heart malformation. Here, we report a genetic rescue of their
heart development by myocardial expression of erbB2 cDNA that allows survival of the mutants to birth. In
rescued erbB2 mutants, Schwann cells are lacking. Motoneurons form and can project to muscle, but nerves
are poorly fasciculated and disorganized. Neuromuscular junctions form, as reflected in clustering of AChR
and postsynaptic expression of the genes encoding the a-AChR, AChE, e-AChR, and the RI subunit of the
cAMP protein kinase. However, a severe loss of motoneurons on cervical and lumbar, but not on thoracic
levels occurs. Our results define the roles of Schwann cells during motoneuron and synapse development, and
reveal different survival requirements for distinct motoneuron populations
2016-03-30T03:18:01Z
2016-03-30T03:18:01Z
1999
Article
Masresha T. Woldeyesus, Stefan Britsch, Dieter Riethmacher, Lan Xu, Eva Sonnenberg-Riethmacher, Faikah Abou-Rebyeh, Richard Harvey, Pico Caroni, Carmen Birchmeier; 1999; Peripheral nervous system defects in erbB2 mutants following genetic rescue of heart development; GENES & DEVELOPMENT
0890-9369/99
http://nur.nu.edu.kz/handle/123456789/1350
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Cold Spring Harbor Laboratory Press
oai:nur.nu.edu.kz:123456789/13512018-08-15T03:49:54Zcom_123456789_104com_123456789_67col_123456789_105
Placental Failure in Mice Lacking the Mammalian Homolog of Glial Cells Missing, GCMa
Riethmacher-Sonnenberg, Eva
Research Subject Categories::NATURAL SCIENCES::Biology::Organism biology::Microbiology
Drosophila melanogaster GCM
Glial Cells Missing
Mammalian Homolog
The GCM family of transcription factors consists of Drosophila melanogaster GCM, an important regulator
of gliogenesis in the fly, and its two mammalian homologs, GCMa and GCMb. To clarify the function of these
mammalian homologs, we deleted GCMa in mice. Genetic ablation of murine GCMa (mGCMa) is embryonic
lethal, with mice dying between 9.5 and 10 days postcoitum. At the time of death, no abnormalities were
apparent in the embryo proper. Nervous system development, in particular, was not impaired, as might have
been expected in analogy to Drosophila GCM. Instead, placental failure was the cause of death. In agreement
with the selective expression of mGCMa in labyrinthine trophoblasts, mutant placentas did not develop a
functional labyrinth layer, which is necessary for nutrient and gas exchange between maternal and fetal blood.
Only a few fetal blood vessels entered the placenta, and these failed to thrive and branch normally. Labyrinthine
trophoblasts did not differentiate. All other layers of the placenta, including spongiotrophoblast and
giant cell layer, formed normally. Our results indicate that mGCMa plays a critical role in trophoblast
differentiation and the signal transduction processes required for normal vascularization of the placenta
2016-03-30T03:36:52Z
2016-03-30T03:36:52Z
2000
Article
Riethmacher-Sonnenberg Eva; 2000; Placental Failure in Mice Lacking the Mammalian Homolog of Glial Cells Missing, GCMa; MOLECULAR AND CELLULAR BIOLOGY
http://nur.nu.edu.kz/handle/123456789/1351
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
American Society for Microbiology
oai:nur.nu.edu.kz:123456789/13522018-08-15T03:49:54Zcom_123456789_104com_123456789_67col_123456789_105
Promotion of periostin expression contributes to the migration of Schwann cells
Sonnenberg-Riethmacher, Eva
Miehe, Michaela
Riethmacher, Dieter
Periostin
Schwann cells
Migration
Neuregulin
Dorsal root ganglia
ECM
Neuregulin ligands and their ErbB receptors are important for the
development of Schwann cells, the glial cells of the peripheral
nervous system (PNS). ErbB3 deficiency is characterized by a
complete loss of Schwann cells along axons of the peripheral nerves,
impaired fasciculation and neuronal cell death. We performed
comparative gene expression analysis of dorsal root ganglia (DRG)
explant cultures from ErbB3-deficient and wild-type mice in order to
identify genes that are involved in Schwann cell development and
migration. The extracellular matrix (ECM) gene periostin was found to
exhibit the most prominent down regulation in ErbB3-deficient DRG.
Expression analysis revealed that the periostin-expressing cell
population in the PNS corresponds to Schwann cell precursors and
Schwann cells, and is particularly high in migratory Schwann cells.
Furthermore, stimulation of Schwann cells with neuregulin-1 (NRG1)
or transforming growth factor β (TGFβ-1) resulted in an upregulation of
periostin expression. Interestingly, DRG explant cultures of periostindeficient
mice revealed a significant reduction of the number of
migrating Schwann cells. These data demonstrate that the
expression of periostin is stimulated by ErbB ligand NRG1 and
influences the migration of Schwann cell precursors
2016-03-30T03:54:07Z
2016-03-30T03:54:07Z
2015
Article
Eva Sonnenberg-Riethmacher, Michaela Miehe, Dieter Riethmacher; 2015; Promotion of periostin expression contributes to the migration of Schwann cells; Journal of Cell Science
3345-3355
http://nur.nu.edu.kz/handle/123456789/1352
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
The Company of Biologists Ltd
oai:nur.nu.edu.kz:123456789/13532018-08-15T03:49:54Zcom_123456789_104com_123456789_67col_123456789_105
The c-ros tvrosine kinase receptor I controls regionalization and differehiation of epithelial cells in the epididymis
Sonnenberg-Riethmacher, Eva
Walter, Barbara
Riethmacher, Dieter
Godecke, Stephanie
Birchmeie, Carmen
c-ros tyrosine kinase receptor
epithelial cells
epididymis
regionalization
terminal differentiation
The c-ros gene was originally identified in mutant form as an oncogene. The proto-oncogene encodes a
tyrosine kinase receptor that is expressed in a small number of epithelial cell types, including those of the
epididymis. Targeted mutations of c-ros in the mouse reveal an essential role of the gene in male fertility.
Male c-ros -1- animals do not reproduce, whereas the fertility of female animals is not affected. We
demonstrate that c-ros is not required in a cell autonomous manner for male germ cell development or
function. The gene, therefore, does not affect sperm generation or function in a direct manner. The primary
defect in the mutant animals was located in the epididymis, showing that c-ros controls appropriate
development of the epithelia, particularly regionalization and terminal differentiation. The epididymal defect
does not interfere with production or storage of sperm but, rather, with sperm maturation and the ability of
sperm to fertilize in vivo. Interestingly, sperm isolated from c-ros - / - animals can fertilize in vitro. Our
results highlight the essential role of the epididymis in male fertility and demonstrate a highly specific
function of the c-ros receptor tyrosine kinase during development of distinct epithelial cells
2016-03-30T04:06:33Z
2016-03-30T04:06:33Z
1996
Article
Eva Sonnenberg-Riethmacher, Barbara Walter, Dieter Riethmacher, Stephanie Godecke, Carmen Birchmeie; 1996; The c-ros tvrosine kinase rece~tor I controls reiionalization and diff erehiation of e~ithelial cells in the epididymis; GENES and DEVELOPMENT
0890-9369196
http://nur.nu.edu.kz/handle/123456789/1353
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Cold Spring Harbor Laboratory Press
oai:nur.nu.edu.kz:123456789/13542018-08-15T03:49:54Zcom_123456789_104com_123456789_67col_123456789_105
Scatter Factor/Hepatocyte Growth Factor and Its Receptor, the c-met Tyrosine Kinase, Can Mediate a Signal Exchange between Mesenchyme and Epithelia during Mouse Development
Sonnenberg-Riethmacher, Eva
Scatter factor/hepatocyte growth factor
Scatter factor/hepatocyte growth factor
(SF/HGF) has potent motogenic, mitogenic, and morphogenetic
activities on epithelial cells in vitro. The cell
surface receptor for this factor was recently identified:
it is the product of the c - m e t protooncogene, a
receptor-type tyrosine kinase. We report here the
novel and distinct expression patterns of SF/HGF and
its receptor during mouse development, which was determined
by a combination of in situ hybridization and
RNase protection experiments. Predominantly, we detect
transcripts of c - m e t in epithelial cells of various
developing organs, whereas the ligand is expressed in
distinct mesenchymal cells in close vicinity. In addition,
transient SF/HGF and c - m e t expression is found
at certain sites of muscle formation; transient expression
of the c - m e t gene is also detected in developing
motoneurons. SF/HGF and the c-met receptor might
thus play multiple developmental roles, most notably,
mediate a signal given by mesenchyme and received
by epithelial. Mesenchymal signals are known to govern
differentiation and morphogenesis of many epithelia,
but the molecular nature of the signals has remained
poorly understood. Therefore, the known
biological activities of SF/HGF in vitro and the embryonal
expression pattern reported here indicate that
this mesenchymal factor can transmit morphogenetic
signals in epithelial development and suggest a molecular
mechanism for mesenchymal epithelial interactions.
2016-03-30T04:21:58Z
2016-03-30T04:21:58Z
1993
Article
Eva Sonnenberg-Riethmacher; 1993; Scatter Factor/Hepatocyte Growth Factor and Its Receptor, the c-met Tyrosine Kinase, Can Mediate a Signal Exchange between Mesenchyme and Epithelia during Mouse Development; The Journal of Cell Biology
http://nur.nu.edu.kz/handle/123456789/1354
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
The Rockefeller University Press
oai:nur.nu.edu.kz:123456789/13552018-08-15T03:49:54Zcom_123456789_104com_123456789_67col_123456789_105
The extracellular-matrix protein matrilin 2 participates in peripheral nerve regeneration
Malin, Dmitry
Sonnenberg-Riethmacher, Eva
Guseva, Daria
Wagener, Raimund
Aszódi, Attila
Irintchev, Audrey
Riethmacher, Dieter
Schwann cells
Axonal outgrowth
Extracellular matrix
Coating substrates
Cell migration
Peripheral nerve regeneration
Matrilins are adaptor proteins of the extracellular matrix
involved in the formation of both collagen-dependent and
collagen-independent filamentous networks. Although their
molecular structure and binding partners have been
characterized, the functional roles of the four matrilin family
members in vivo are still largely unknown. Here, we show that
matrilin 2, expressed in pre-myelinating Schwann cells during
normal development, profoundly influences the behaviour of
glial cells and neurons in vitro. When offered as a uniform
substrate, matrilin 2 increased neurite outgrowth of dorsal root
ganglia (DRG) neurons and enhanced the migration of both cell
line- and embryonic DRG-derived Schwann cells. Vice versa,
axonal outgrowth and cell migration were decreased in DRG
cultures prepared from matrilin-2-deficient mice compared with
wild-type (wt) cultures. In stripe assays, matrilin 2 alone was
sufficient to guide axonal growth and, interestingly, axons
favoured the combination of matrilin 2 and laminin over
laminin alone. In vivo, matrilin 2 was strongly upregulated in
injured peripheral nerves of adult wild-type mice and failure
of protein upregulation in knockout mice resulted in delayed
regrowth of regenerating axons and delayed time-course of
functional recovery. Strikingly, the functional recovery 2 months
after nerve injury was inferior in matrilin-2-deficient mice
compared with wild-type littermates, although motoneuron
survival, quality of axonal regeneration, estimated by analyses
of axonal diameters and degrees of myelination, and Schwann
cell proliferation were not influenced by the mutation. These
results show that matrilin 2 is a permissive substrate for axonal
growth and cell migration, and that it is required for successful
nerve regeneration
2016-03-30T04:33:44Z
2016-03-30T04:33:44Z
2009
Article
Dmitry Malin, Eva Sonnenberg-Riethmacher, Daria Guseva, Raimund Wagener, Attila Aszódi, Audrey Irintchev, Dieter Riethmacher; 2009; The extracellular-matrix protein matrilin 2 participates in peripheral nerve regeneration; Journal of Cell Science
http://nur.nu.edu.kz/handle/123456789/1355
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
The Company of Biologists
oai:nur.nu.edu.kz:123456789/14582018-08-15T03:49:53Zcom_123456789_104com_123456789_67col_123456789_105
Honey: an immunomodulatory agent for disorders of the skin
McLoone, Pauline
Warnock, Mary
Fyfe, Lorna
honey
immunomodulatory
skin disorders
wound healing
Studies have shown that honeys from around the world can inhibit the growth of a range of dermatologically important microbes. As well as reports of the antimicrobial properties of honey, a number of recent in vitro and in vivo studies suggest that honey is able to modulate immunological parameters related to the skin immune system. Paradoxically, both immune-stimulatory and anti-inflammatory effects have been observed. In this review, scientific research investigating the immunomodulatory properties of honeys from around the world, in relation to disorders of the skin, is evaluated. Whilst there is sufficient evidence to suggest that honey does indeed have immunomodulatory properties, which may at least partially explain the ability of honey to promote the healing of wounds, there are still gaps in the scientific knowledge and literature. More research is necessary for a more complete understanding of the immune modulating properties of honey and to enable the utilisation of honey as an immune-modulating agent in dermatology
2016-05-11T03:50:04Z
2016-05-11T03:50:04Z
2016-04
Article
Pauline McLoone, Mary Warnock, Lorna Fyfe; 2016; Honey: an immunomodulatory agent for disorders of the skin; Journal of Microbiology, Immunology and Infection
http://nur.nu.edu.kz/handle/123456789/1458
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
oai:nur.nu.edu.kz:123456789/14592018-08-15T03:50:25Zcom_123456789_104com_123456789_67col_123456789_105
Honey: a realistic antimicrobal for disorders of the skin
McLoone, Pauline
Warnock, Mary
Fyfe, Lorna
Anti-microbial
dermatology
honey
wound infections
Resistance of pathogenic micro-organisms to antibiotics is a serious global health concern. In this review, research investigating the antimicrobial properties of honeys from around the world, against skin relevant microbes is evaluated. A plethora of in vitro studies have revealed that honeys from all over the world have potent microbicidal activity against dermatologically important microbes. Moreover, in vitro studies have shown that honey can reduce microbial pathogenicity as well as reverse anti-microbial resistance. Studies investigating the antimicrobial properties of honey in vivo have been more controversial. It is evident that innovative research is required to exploit the anti-microbial properties of honey for clinical use and to determine the efficacy of honey in the treatment of a range of skin disorders with a microbiological aetiology
2016-05-11T04:03:49Z
2016-05-11T04:03:49Z
2016-04
Article
Pauline McLoone, Mary Warnock, Lorna Fyfe; 2016; Honey: a realistic antimicrobal for disorders of the skin; Journal of Microbiology, Immunology and Infection
http://nur.nu.edu.kz/handle/123456789/1459
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
oai:nur.nu.edu.kz:123456789/19002018-08-15T03:50:05Zcom_123456789_104com_123456789_67col_123456789_105
A novel PKD1 variant demonstrates a disease-modifying role in trans with a truncating PKD1 mutation in patients with Autosomal Dominant Polycystic Kidney Disease
Ali, Hamad
Hussain, Naser
Naim, Medhat
Zayed, Mohamed
Al-Mulla, Fahd
Kehinde, Elijah
Seaburg, Lauren
Sundsbak, Jamie
Harris, Peter
polycystic kidney disease
ADPKD
PKD1
ESRD
Novel variant
Genetic modifier
eGFR
PKD1
Research Subject Categories::MEDICINE
Autosomal Dominant Polycystic Kidney Disease (ADPKD) is the most common form of Polycystic Kidney Disease (PKD) and occurs at a frequency of 1/800 to 1/1000 affecting all ethnic groups worldwide. ADPKD shows significant intrafamilial phenotypic variability in the rate of disease progression and extra-renal manifestations, which suggests the involvement of heritable modifier genes. Here we show that the PKD1 gene can act as a
disease causing and a disease modifier gene in ADPKD patients.
2016-11-21T05:44:30Z
2016-11-21T05:44:30Z
2015
Article
http://nur.nu.edu.kz/handle/123456789/1900
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Ali et al. BMC Nephrology
oai:nur.nu.edu.kz:123456789/19012018-08-15T03:49:55Zcom_123456789_104com_123456789_67col_123456789_105
Rapid liquid chromatography–tandem mass spectrometry method for the determination of a broad mixture of pharmaceuticals in surface water
Conley, Justin
Symes, Steven
Kindelberger, Scott
Richards, Sean
ultra performance liquid chromatography
solid-phase extraction
tandem mass spectrometry
environmental analysis
surface water
pharmaceutical
Herein, a new method for the detection of 13 different pharmaceuticals and one metabolite in surface water at low ng/L levels is described. The method utilizes ultra performance liquid chromatography–tandem mass spectrometry and a solid-phase extraction sample preparation. Mean method detection limits were low (4.10 ng/L) and overall solid-phase extraction recovery and reproducibility was adequate (mean recovery, 77.9%; mean RSD, 7.3%). The method allows for quick run times and minimal solvent use as compared with other previously reported high performance
liquid chromatography-based methods. Application of this method for the detection of pharmaceuticals in Tennessee River surfacewater determined
that caffeine, sulfamethoxazole, and carbamazepine were frequently detected (100% of samples). Trimethoprim was moderately detected (30% of samples); acetaminophen, atorvastatin, and lovastatin were infrequently detected (10% of samples); and ciprofloxacin, diltiazem, fluoxetine, levofloxacin, norfluoxetine, ranitidine, and sertraline were not detected. This study reports the first detection of lovastatin in surface water.
2016-11-21T05:51:12Z
2016-11-21T05:51:12Z
2008
Article
http://nur.nu.edu.kz/handle/123456789/1901
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Journal of Chromatography A. www.sciencedirect.com
oai:nur.nu.edu.kz:123456789/19022018-08-15T03:50:00Zcom_123456789_104com_123456789_67col_123456789_105
ANURIA SECONDARY TO HOT WEATHER-INDUCED HYPERURICAEMIA: DIAGNOSIS AND MANAGEMENT
Al-Tawheed, Adel R.
Al-Awadi, Khaleel A.
Kehinde, Elijah O.
Abdul-Halim, Hamdy
Al-Hunayan, Adel
Ali, Yousef
Mohammed, Akram H.
hot weather
anuria
hyperuricaemia
uric acid nephropathy
Research Subject Categories::MEDICINE
There is little information on the management of anuria secondary to severe
volume depletion or as a rare manifestation of heat stroke in areas of the world with very hot
summers. We present our experience with hot weather-induced hyperuricaemia in Kuwait.
2016-11-21T05:59:44Z
2016-11-21T05:59:44Z
2003-09
Article
http://nur.nu.edu.kz/handle/123456789/1902
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Annals of Saudi Medicine
oai:nur.nu.edu.kz:123456789/19032018-08-15T03:50:01Zcom_123456789_104com_123456789_67col_123456789_105
Comparison of the sensitivity and specificity of urine cytology, urinary nuclear matrix protein-22 and multitarget fluorescence in situ hybridization assay in the detection of bladder cancer
Kehinde, Elijah
Al-Mulla, Fahd
Kapila, Kusum
Anim, Jehoram T.
Bladder cancer
diagnosis
NMP22
urine cytology
UroVysion FISH
Research Subject Categories::MEDICINE
This study aimed to compare the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of urine cytology, BladderChek nuclear matrix protein-22 (NMP22) and UroVysion fluorescence in situ hybridization (FISH) tests in patients with newly diagnosed bladder cancer, those with recurrent bladder cancer, and those with bladder cancer but in remission during surveillance. Material and methods. Voided urine samples obtained from 178 patients with suspected or known bladder cancer about to undergo diagnostic or surveillance cystoscopy and 25 control
subjects without the disease were divided into four and used for urine culture and cytology, NMP22 BladderChek and UroVysion FISH tests.
2016-11-21T08:41:39Z
2016-11-21T08:41:39Z
2010
Article
http://nur.nu.edu.kz/handle/123456789/1903
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Scandinavian Journal of Urology and Nephrology
oai:nur.nu.edu.kz:123456789/22432019-08-08T06:59:15Zcom_123456789_104com_123456789_67col_123456789_105
A roadmap for offering MOOC from an LMIC institution
Abidi, Syed H
Pasha, Aamna
Moran, Greg
Ali, Syed
distance learning
LMIC
MOOC
MOOCs are massive open online courses that are globally accessible, free of charge. Given their cost-free and open accessibility, it is surprising that only a few institutions have offered MOOCs from low- and middle-income countries (LMICs). Pakistan recently made this short list of LMICs as the first two MOOCs were launched from the country, in 2014 and 2016. Drawing from that experience, the organizers of that course present a roadmap for LMIC institutions for developing a MOOC, focusing especially on the technological and pedagogical limitations that an LMIC institution might find deterring.
2017-01-12T06:12:49Z
2017-01-12T06:12:49Z
2016-07-20
Article
Abidi, S. H., Pasha, A., Moran, G., & Ali, S. (2016). A roadmap for offering MOOC from an LMIC institution. Learning, Media and Technology, 1-6. DOI: 10.1080/17439884.2016.1205601
http://nur.nu.edu.kz/handle/123456789/2243
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Learning, Media and Technology
oai:nur.nu.edu.kz:123456789/22442018-08-15T03:50:02Zcom_123456789_104com_123456789_67col_123456789_105
Cutting edge: IgE plays an active role in tumor immunosurveillance in mice
Nigro, Elisa A.
Brini, Anna T.
Yenagi, Vijay A.
Ferreira, Lorena M.
Achatz-Straussberger, Gertrude
Ambrosi, Alessandro
Sanvito, Francesca
Soprana, Elisa
Van Anken, Eelco
Achatz, Gernot
Siccardi, Antonio G.
Vangelista, Luca
neoplasms
immunoglobulin E
immunologic monitoring
Exogenous IgE acts as an adjuvant in tumor vaccination in mice, and therefore a direct role of endogenous IgE in tumor immunosurveillance was investigated. By using genetically engineered mice, we found that IgE ablation rendered mice more susceptible to the growth of transplantable tumors. Conversely, a strengthened IgE response provided mice with partial or complete resistance to tumor growth, depending on the tumor type. By genetic crosses, we showed that IgE-mediated tumor protection was mostly lost in mice lacking FceRI. Tumor protection was also lost after depletion of CD8+ T cells, highlighting a cross-Talk between IgE and T cell- mediated tumor immunosurveillance. Our findings provide the rationale for clinical observations that relate atopy with a lower risk for developing cancer and open new avenues for the design of immunotherapeutics relevant for clinical oncology. The Journal of Immunology, 2016, 197: 2583-2588.
2017-01-12T06:26:47Z
2017-01-12T06:26:47Z
2016-10-01
Article
Nigro, E. A., Brini, A. T., Yenagi, V. A., Ferreira, L. M., Achatz-Straussberger, G., Ambrosi, A., ... Vangelista, L. (2016). Cutting edge: IgE plays an active role in tumor immunosurveillance in mice. Journal of Immunology, 197(7), 2583-2588. DOI: 10.4049/jimmunol.1601026
http://nur.nu.edu.kz/handle/123456789/2244
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Journal of Immunology
oai:nur.nu.edu.kz:123456789/22452018-08-15T03:50:09Zcom_123456789_104com_123456789_67col_123456789_105
First Case in Kazakhstan of Successful Therapy With 2 Consecutive Direct-Acting Antiviral Regimens in a Patient with Hepatitis C Virus-Induced Decompensated Liver Cirrhosis on a Liver Transplant Wait List
Yesmembetov, Kakharman
Ashimkhanova, Aiymkul
Kaliaskarova, Kulpash
Kazakhstan
hepacivirus
liver cirrhosis
A 40-year-old man, diagnosed with decompensated liver cirrhosis because of hepatitis C virus, was on the wait list for a liver transplant when he began treatment with the direct-acting antivirals simeprevir 150 mg and sofosbuvir 400 mg. The patient demonstrated end of treatment virologic response at week 12, normal bilirubin, and alanine aminotransferase levels, resolution of ascites, with downgrading to subcompensated liver cirrhosis, and was removed from the liver transplant wait list. However, the patient did not comply with the recommended duration of the antiviral treatment of at least 16 weeks, which resulted in hepatitis C virus relapse at posttreatment week 12. Later, the patient started an alternative regimen that included a combination of ombitasvir 12.5 mg, paritaprevir 75 mg, ritonavir 50 mg, and dasabuvir 250 mg for 24 weeks and achieved a sustained virologic response. However, despite undetectable hepatitis C virus, the patient began to deteriorate again and was again put on the liver transplant wait list. This first described clinical case in Kazakhstan of successful antiviral therapy with 2 consecutive directacting agents demonstrates the importance of virus eradication of pretransplant survival extension and delaying the need for liver transplant.
2017-01-12T06:43:05Z
2017-01-12T06:43:05Z
2016-11-14
Article
Yesmembetov, K., Ashimkhanova, A., & Kaliaskarova, K. (2016). First Case in Kazakhstan of Successful Therapy With 2 Consecutive Direct-Acting Antiviral Regimens in a Patient with Hepatitis C Virus-Induced Decompensated Liver Cirrhosis on a Liver Transplant Wait List. Experimental and Clinical Transplantation, 14(Suppl 3), 112-113.
http://nur.nu.edu.kz/handle/123456789/2245
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Experimental and Clinical Transplantation
oai:nur.nu.edu.kz:123456789/22462018-08-15T03:50:02Zcom_123456789_104com_123456789_67col_123456789_105
Food authentication: Techniques, trends & emerging approaches
Danezis, Georgios P.
Tsagkaris, Aristidis S.
Camin, Federica
Brusic, Vladimir
Georgiou, Constantinos A.
adulteration
analytical techniques
authenticity
botanical origin
food authentication
fraud
geographical origin
scientometric evaluation
species
Food authentication is a rapidly growing field due to increasing public awareness concerning food quality and safety. This review presents critically the analytical techniques which are used for authenticity assessment, explaining how and why they give plausible solutions. Classification of different methodologies is based on authenticity indicators providing insight into future developments. Analytical breakthroughs and novel techniques that emerged recently are discussed, along with their applications on food authentication. We have discussed current limits and gaps, related to informatics needs for data analysis of large quantities. Reporting standards and reference database are elaborated indicating urgent needs for the progress of this field. A scientometric evaluation highlighted the research trends and emerging approaches of this evolving field. Popular analytical techniques are commented, while the potential of the field is depicted in the temporal evolution of the research output focusing on geographical distribution of research activity and preferred journals used for dissemination.
2017-01-12T08:03:18Z
2017-01-12T08:03:18Z
2016
Article
Danezis, G. P., Tsagkaris, A. S., Camin, F., Brusic, V., & Georgiou, C. A. (2016). Food authentication: Techniques, trends & emerging approaches. TrAC - Trends in Analytical Chemistry. DOI: 10.1016/j.trac.2016.02.026
http://nur.nu.edu.kz/handle/123456789/2246
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
TrAC - Trends in Analytical Chemistry
oai:nur.nu.edu.kz:123456789/22472018-08-15T03:50:04Zcom_123456789_104com_123456789_67col_123456789_105
Risk factors for children's blood lead levels in metal mining and smelting communities in Armenia: A cross-sectional study
Grigoryan, Ruzanna
Petrosyan, Varduhi
Melkom Melkomian, Dzovinar
Khachadourian, Vahe
McCartor, Andrew
Crape, Byron
blood lead level
children
lead contamination
lead exposure
metal mining
smelting
Background: Children's exposure to lead poses a significant risk for neurobehavioral consequences. Existing studies documented lead contamination in residential soil in mining and smelting communities in Armenia. This study aimed to assess blood lead levels (BLL) in children living in three communities in Armenia adjacent to metal mining and smelting industries, and related risk factors. Methods: This cross-sectional study included 159 children born from 2007 to 2009 and living in Alaverdi and Akhtala communities and Erebuni district in Yerevan - the capital city. The BLL was measured with a portable LeadCare II Blood Lead Analyzer; a survey was conducted with primary caregivers. Results: Overall Geometric Mean (GM) of BLL was 6.0 μg/dl: 6.8 for Akhtala, 6.4 for Alaverdi and 5.1 for Yerevan. In the sample 68.6 % of children had BLL above CDC defined reference level of 5 μg/dl: 83.8 % in Akhtala, 72.5 % in Alaverdi, and 52.8 % in Yerevan. Caregiver's lower education, dusting furniture less than daily, and housing distance from toxic source(s) were risk factors for higher BLL. Additional analysis for separate communities demonstrated interaction between housing distance from toxic source(s) and type of window in Erebuni district of Yerevan. Conclusions: The study demonstrated that children in three communities adjacent to metal mining and smelting industries were exposed to lead. Investigation of the risk factors suggested that in addition to promoting safe industrial practices at the national level, community-specific interventions could be implemented in low- and middle-income countries to reduce BLL among children.
2017-01-12T08:12:55Z
2017-01-12T08:12:55Z
2016-09-07
Article
Grigoryan, R., Petrosyan, V., Melkom Melkomian, D., Khachadourian, V., McCartor, A., & Crape, B. (2016). Risk factors for children's blood lead levels in metal mining and smelting communities in Armenia: A cross-sectional study. BMC Public Health, 16(1), [945]. DOI: 10.1186/s12889-016-3613-9
http://nur.nu.edu.kz/handle/123456789/2247
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
BMC Public Health
oai:nur.nu.edu.kz:123456789/22482018-08-15T03:50:02Zcom_123456789_104com_123456789_67col_123456789_105
Risk factors of postoperative complications after radical cystectomy with continent or conduit urinary diversion in Armenia
Tsaturyan, Arman
Petrosyan, Varduhi
Crape, Byron
Sahakyan, Yeva
Abrahamyan, Lusine
bladder cancer
complications
mortality
radical cystectomy
risk factors
To estimate the surgical volume and the incidence of in-hospital complications of RC in Armenia from 2005 to 2012, and to investigate potential risk factors of complications. The study utilized a retrospective chart review in a cohort of patients who had RC followed by either continent or conduit urinary diversion in all hospitals of Armenia from 2005 to 2012. A detailed chart review was conducted abstracting information on baseline demographic and clinical characteristics, surgical procedural details, postoperative management and in-hospital complications. Multivariable logistic regression analysis was applied to estimate the independent risk factors for developing ‘any postoperative complication’. The total study sample included 273 patients (mean age = 58.5 years, 93.4 % men). Overall, 28.9 % (n = 79) of patients had at least one in-hospital complication. The hospital mortality rate was 4.8 % (n = 13). The most frequent types of complications were wound-related (10.3 %), gastrointestinal (9.2 %) and infectious (7.0 %). The ischemic heart disease (OR = 3.3, 95 % CI 1.5–7.4), perioperative transfusion (OR = 2.0, 1.1–3.6), glucose level [OR = 0.71 (0.63–0.95)], and hospital type (OR = 2.3, 95 % CI 1.1–4.7) were independent predictors of postoperative complications. The rate of RC complications in Armenia was similar to those observed in other countries. Future prospective studies should evaluate the effect of RC complications on long-term outcomes and costs in Armenia. Policy recommendations should address the issues regarding surgeon training and hospital volume to decrease the risk of RC complications.
2017-01-12T08:22:58Z
2017-01-12T08:22:58Z
2016-12-01
Article
Tsaturyan, A., Petrosyan, V., Crape, B., Sahakyan, Y., & Abrahamyan, L. (2016). Risk factors of postoperative complications after radical cystectomy with continent or conduit urinary diversion in Armenia. SpringerPlus, 5(1), 1-9. [134]. DOI: 10.1186/s40064-016-1757-9
http://nur.nu.edu.kz/handle/123456789/2248
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
SpringerPlus
oai:nur.nu.edu.kz:123456789/22492018-08-15T03:50:09Zcom_123456789_104com_123456789_67col_123456789_105
Sebaceous lipids are essential for water repulsion, protection against UVB-induced apoptosis and ocular integrity in mice
Dahlhoff, Maik
Camera, Emanuela
Schäfer, Matthias
Emrich, Daniela
Riethmacher, Dieter
Foster, April
Paus, Ralf
Schneider, Marlon R.
gene targeting
meibomian gland disease
mouse
sebaceous glands
water repulsion
Sebocytes, which are characterized by lipid accumulation that leads to cell disruption, can be found in hair follicle-associated sebaceous glands (SGs) or in free SGs such as the Meibomian glands in the eyelids. Because genetic tools that allow targeting of sebocytes while maintaining intact epidermal lipids are lacking, the relevance of sebaceous lipids in health and disease remains poorly understood. Using Scd3, which is expressed exclusively in mature sebocytes, we established a mouse line with sebocyte-specific expression of Cre recombinase. Both RT-PCR analysis and crossing into Rosa26-lacZ reporter mice and KrasG12D mice confirmed Cre activity specifically in SGs, with no activity in other skin compartments. Importantly, loss of SCD3 function did not cause detectable phenotypical alterations, endorsing the usefulness of Scd3-Cre mice for further functional studies. Scd3-Cre-induced, diphtheria chain A toxin-mediated depletion of sebaceous lipids resulted in impaired water repulsion and thermoregulation, increased rates of UVB-induced epidermal apoptosis and caused a severe pathology of the ocular surface resembling Meibomian gland dysfunction. This novel mouse line will be useful for further investigating the roles of sebaceous lipids in skin and eye integrity.
2017-01-12T08:33:10Z
2017-01-12T08:33:10Z
2016-05-15
Article
Dahlhoff, M., Camera, E., Schäfer, M., Emrich, D., Riethmacher, D., Foster, A., ... Schneider, M. R. (2016). Sebaceous lipids are essential for water repulsion, protection against UVB-induced apoptosis and ocular integrity in mice. Development (Cambridge), 143(10), 1823-1831. DOI: 10.1242/dev.132753
http://nur.nu.edu.kz/handle/123456789/2249
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Development (Cambridge)
oai:nur.nu.edu.kz:123456789/22502018-08-15T03:50:07Zcom_123456789_104com_123456789_67col_123456789_105
An adaptive genetic algorithm for selection of blood-based biomarkers for prediction of Alzheimer's disease progression
Vandewater, Luke
Brusic, Vladimir
Wilson, William
Macaulay, Lance
Zhang, Ping
adaptive genetic algorithm
Alzheimer's
biomarkers
logistic regression
prediction
Background: Alzheimer's disease is a multifactorial disorder that may be diagnosed earlier using a combination of tests rather than any single test. Search algorithms and optimization techniques in combination with model evaluation techniques have been used previously to perform the selection of suitable feature sets. Previously we successfully applied GA with LR to neuropsychological data contained within the The Australian Imaging, Biomarkers and Lifestyle (AIBL) study of aging, to select cognitive tests for prediction of progression of AD. This research addresses an Adaptive Genetic Algorithm (AGA) in combination with LR for identifying the best biomarker combination for prediction of the progression to AD. Results: The model has been explored in terms of parameter optimization to predict conversion from healthy stage to AD with high accuracy. Several feature sets were selected - the resulting prediction moddels showed higher area under the ROC values (0.83-0.89). The results has shown consistency with some of the medical research reported in literature. Conclusion: The AGA has proven useful in selecting the best combination of biomarkers for prediction of AD progression. The algorithm presented here is generic and can be extended to other data sets generated in projects that seek to identify combination of biomarkers or other features that are predictive of disease onset or progression.
2017-01-12T08:43:23Z
2017-01-12T08:43:23Z
2015-12-09
Article
Vandewater, L., Brusic, V., Wilson, W., Macaulay, L., & Zhang, P. (2015). An adaptive genetic algorithm for selection of blood-based biomarkers for prediction of Alzheimer's disease progression. BMC Bioinformatics, 16(18), [S1]. DOI: 10.1186/1471-2105-16-S18-S1
http://nur.nu.edu.kz/handle/123456789/2250
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
BMC Bioinformatics
oai:nur.nu.edu.kz:123456789/22512018-08-15T03:50:27Zcom_123456789_104com_123456789_67col_123456789_105
A systematic analysis of a broadly neutralizing antibody AR3C epitopes on Hepatitis C virus E2 envelope glycoprotein and their cross-reactivity
Sun, Jing
Brusic, Vladimir
neutralizing antibodies
hepacivirus
epitopes
proteins
Background: Hepatitis C virus (HCV) belongs to Flaviviridae family of viruses. HCV represents a major challenge to public health since its estimated global prevalence is 2.8% of the world's human population. The design and development of HCV vaccine has been hampered by rapid evolution of viral quasispecies resulting in antibody escape variants. HCV envelope glycoprotein E1 and E2 that mediate fusion and entry of the virus into host cells are primary targets of the host immune responses. Results: Structural characterization of E2 core protein and a broadly neutralizing antibody AR3C together with E1E2 sequence information enabled the analysis of B-cell epitope variability. The E2 binding site by AR3C and its surrounding area were identified from the crystal structure of E2c-AR3C complex. We clustered HCV strains using the concept of 'discontinuous motif/peptide' and classified B-cell epitopes based on their similarity. Conclusions: The assessment of antibody neutralizing coverage provides insights into potential cross-reactivity of the AR3C neutralizing antibody across a large number of HCV variants.
2017-01-12T08:58:19Z
2017-01-12T08:58:19Z
2015-12-09
Article
Sun, J., & Brusic, V. (2015). A systematic analysis of a broadly neutralizing antibody AR3C epitopes on Hepatitis C virus E2 envelope glycoprotein and their cross-reactivity. BMC Medical Genomics, 8(4), [571]. DOI: 10.1186/1755-8794-8-S4-S6
http://nur.nu.edu.kz/handle/123456789/2251
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
BMC Medical Genomics
oai:nur.nu.edu.kz:123456789/22522018-08-15T03:50:08Zcom_123456789_104com_123456789_67col_123456789_105
Biomarkers and signaling pathways of colorectal cancer stem cells
Abetov, Danysh
Mustapova, Zhanar
Saliev, Timur
Bulanin, Denis
biomarkers
carcinogenesis
colorectal cancer
signaling pathways
stem cells
The progression of colorectal cancer is commonly characterized by accumulation of genetic or epigenetic abnormalities, altering regulation of gene expression as well as normal protein structures and functions. Nonetheless, there are some questions that remain to be elucidated, such as the origin of cancer cells and populations of cells initiating and propagating tumor development. Currently, there are two rival theories describing the process of carcinogenesis. One is the stochastic model, arguing that any cell is capable of initiating and triggering the development of cancer. Meanwhile, the cancer stem cell model hypothesizes that only a small fraction of stem cells possesses cancer-promoting properties. Typically, colorectal cancer stem cells (CSCs) share the same molecular signaling profiles with normal stem cells or embryonic stem cells, such as Wnt, Notch, TGF-β, and Hedgehog. Nevertheless, CSCs differ from normal stem cells and the bulk of tumor cells in their tumorigenic potential and susceptibility to chemotherapeutic drugs. This may be a possible explanation of the high percentage of cancer recurrence in patients who underwent chemotherapeutic treatment and surgery. This review article focuses on the colorectal cancer stem cell biomarkers and the role of upregulated signaling pathways implicated in the initiation and progression of colorectal cancer.
2017-01-12T09:07:12Z
2017-01-12T09:07:12Z
2015-03-01
Article
Abetov, D., Mustapova, Z., Saliev, T., & Bulanin, D. (2015). Biomarkers and signaling pathways of colorectal cancer stem cells. Tumor Biology, 36(3), 1339-1353. DOI: 10.1007/s13277-015-3198-4
http://nur.nu.edu.kz/handle/123456789/2252
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Tumor Biology
oai:nur.nu.edu.kz:123456789/22532018-08-15T03:50:06Zcom_123456789_104com_123456789_67col_123456789_105
Epidermal growth factor receptor immunohistochemistry: New opportunities in metastatic colorectal cancer
Hutchinson, Ryan A.
Adams, Richard A.
McArt, Darragh G.
Salto-Tellez, Manuel
Jasani, Bharat
Hamilton, Peter W.
epidermal growth factor receptor
heterogeneity
image analysis
immunohistochemistry
localisation
metastatic colorectal cancer
personalised medicine
The treatment of cancer is becoming more precise, targeting specific oncogenic drivers with targeted molecular therapies. The epidermal growth factor receptor has been found to be over-expressed in a multitude of solid tumours. Immunohistochemistry is widely used in the fields of diagnostic and personalised medicine to localise and visualise disease specific proteins. To date the clinical utility of epidermal growth factor receptor immunohistochemistry in determining monoclonal antibody efficacy has remained somewhat inconclusive. The lack of an agreed reproducible scoring criteria for epidermal growth factor receptor immunohistochemistry has, in various clinical trials yielded conflicting results as to the use of epidermal growth factor receptor immunohistochemistry assay as a companion diagnostic. This has resulted in this test being removed from the licence for the drug panitumumab and not performed in clinical practice for cetuximab. In this review we explore the reasons behind this with a particular emphasis on colorectal cancer, and to suggest a way of resolving the situation through improving the precision of epidermal growth factor receptor immunohistochemistry with quantitative image analysis of digitised images complemented with companion molecular morphological techniques such as in situ hybridisation and section based gene mutation analysis.
2017-01-12T09:18:13Z
2017-01-12T09:18:13Z
2015-07-07
Article
Hutchinson, R. A., Adams, R. A., McArt, D. G., Salto-Tellez, M., Jasani, B., & Hamilton, P. W. (2015). Epidermal growth factor receptor immunohistochemistry: New opportunities in metastatic colorectal cancer. Journal of Translational Medicine, 13(1), [217]. DOI: 10.1186/s12967-015-0531-z
http://nur.nu.edu.kz/handle/123456789/2253
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Journal of Translational Medicine
oai:nur.nu.edu.kz:123456789/22542018-08-15T03:50:07Zcom_123456789_104com_123456789_67col_123456789_105
Impact of updated HER2 testing guidelines in breast cancer - Re-evaluation of HERA trial fluorescence in situ hybridization data
Stoss, Oliver C.
Scheel, Andreas
Nagelmeier, Iris
Schildhaus, Hans Ulrich
Henkel, Thomas
Viale, Giuseppe
Jasani, Bharat
Untch, Michael
Rüschoff, Josef
practice guidelines
breast neoplasms
decision making
Recently the American Society of Clinical Oncology and the College of American Pathologists have updated their clinical practice guidelines for HER2 testing in breast cancer. In order to evaluate these new recommendations, we have re-assessed the HER2 status of 6018 breast cancer cases of the screening population for the HERceptin adjuvant (HERA) trial that were originally centrally tested by fluorescence in situ hybridization based on the FDA-released test guidelines. According to the most recent 2013 ASCO/CAP recommendations, 3380 (56.2%) cases were classified as HER2 positive compared with 3359 (55.8%) applying the HERA/FDA scheme and 3339 (55.5%) applying the 2007 ASCO/CAP guidelines. Twenty-one cases switched from negative (HERA/FDA scheme) to positive (2013 ASCO/CAP guidelines). This group is characterized by a mean HER2 gene copy number of ≥6.0, polysomy or co-amplification of CEP17 with an average CEP17 count of 5, and with HER2 receptor overexpression in 75% of cases. On the basis of the HER2 gene copy number alone, we observe 494 cases (8.2%) that are in the equivocal range. Most of these cases (>80%) were also nondecisive by immunohistochemistry (score 2+) irrespective of whether ratio was . The number of equivocal cases that would require HER2 reflex testing decreases to 113 (1.9%) if in addition to the HER2 gene copy number also the ratio of HER2 and CEP17 copy numbers is considered via dual-color in situ hybridization. The combination of applying the HER2 mean gene copy number as well as the HER2/CEP17 ratio to define equivocal test decisions by fluorescence in situ hybridization as proposed by the current ASCO/CAP guidelines appears to be a more optimum approach to adopt in order to avoid or minimize reporting of false negative results. Using the mean HER2 gene copy number alone for decision making results in a significant increase of equivocal cases.
2017-01-12T09:30:55Z
2017-01-12T09:30:55Z
2015-12-01
Article
Stoss, O. C., Scheel, A., Nagelmeier, I., Schildhaus, H. U., Henkel, T., Viale, G., ... Rüschoff, J. (2015). Impact of updated HER2 testing guidelines in breast cancer - Re-evaluation of HERA trial fluorescence in situ hybridization data. Modern Pathology, 28(12), 1528-1534. DOI: 10.1038/modpathol.2015.112
http://nur.nu.edu.kz/handle/123456789/2254
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Modern Pathology
oai:nur.nu.edu.kz:123456789/22552018-08-15T03:50:08Zcom_123456789_104com_123456789_67col_123456789_105
Management of super-refractory status epilepticus with isoflurane and hypothermia
Zhumadilov, Agzam
Gilman, Charles P.
Viderman, Dmitriy
epilepsy
hypothermia
isoflurane
neurocritical care
neurotrauma
SRSE
Super-refractory status epilepticus (SRSE) is defined as status epilepticus that continues 24 h or more after the onset of anesthesia, and includes those cases in which epilepsy is recurrent upon treatment reduction. We describe the presentation and successful management of a male patient with SRSE using the inhaled anesthetic isoflurane, and mild hypothermia (HT). The potential utility of combined HT and volatile anesthesia is discussed.
2017-01-12T09:38:56Z
2017-01-12T09:38:56Z
2015
Article
Zhumadilov, A., Gilman, C. P., & Viderman, D. (2015). Management of super-refractory status epilepticus with isoflurane and hypothermia. Frontiers in Neurology, 6(JAN), [286]. DOI: 10.3389/fneur.2014.00286
http://nur.nu.edu.kz/handle/123456789/2255
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Frontiers in Neurology
oai:nur.nu.edu.kz:123456789/22562018-08-15T03:50:07Zcom_123456789_104com_123456789_67col_123456789_105
Novel Small Molecule Inhibitors of Cancer Stem Cell Signaling Pathways
Abetov, Danysh
Mustapova, Zhanar
Saliev, Timur
Bulanin, Denis
Batyrbekov, Kanat
Gilman, Charles P.
cancer
hedgehog
inhibitor
notch
signaling pathway
stem cells
wnt
The main aim of oncologists worldwide is to understand and then intervene in the primary tumor initiation and propagation mechanisms. This is essential to allow targeted elimination of cancer cells without altering normal mitotic cells. Currently, there are two main rival theories describing the process of tumorigenesis. According to the Stochastic Model, potentially any cell, once defunct, is capable of initiating carcinogenesis. Alternatively the Cancer Stem Cell (CSC) Model posits that only a small fraction of undifferentiated tumor cells are capable of triggering carcinogenesis. Like healthy stem cells, CSCs are also characterized by a capacity for self-renewal and the ability to generate differentiated progeny, possibly mediating treatment resistance, thus leading to tumor recurrence and metastasis. Moreover, molecular signaling profiles are similar between CSCs and normal stem cells, including Wnt, Notch and Hedgehog pathways. Therefore, development of novel chemotherapeutic agents and proteins (e.g., enzymes and antibodies) specifically targeting CSCs are attractive pharmaceutical candidates. This article describes small molecule inhibitors of stem cell pathways Wnt, Notch and Hedgehog, and their recent chemotherapy clinical trials.
2017-01-12T09:49:37Z
2017-01-12T09:49:37Z
2015-12-01
Article
Abetov, D., Mustapova, Z., Saliev, T., Bulanin, D., Batyrbekov, K., & Gilman, C. P. (2015). Novel Small Molecule Inhibitors of Cancer Stem Cell Signaling Pathways. Stem Cell Reviews and Reports, 11(6), 909-918. DOI: 10.1007/s12015-015-9612-x
http://nur.nu.edu.kz/handle/123456789/2256
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Stem Cell Reviews and Reports
oai:nur.nu.edu.kz:123456789/22572018-08-15T03:50:07Zcom_123456789_104com_123456789_67col_123456789_105
Bioinformatics for cancer immunotherapy target discovery
Olsen, Lars Rønn
Campos, Benito
Barnkob, Mike Stein
Winther, Ole
Brusic, Vladimir
Andersen, Mads Hald
biological databases
cancer vaccines
computational biology
T cell epitopes
tumor antigens
The mechanisms of immune response to cancer have been studied extensively and great effort has been invested into harnessing the therapeutic potential of the immune system. Immunotherapies have seen significant advances in the past 20 years, but the full potential of protective and therapeutic cancer immunotherapies has yet to be fulfilled. The insufficient efficacy of existing treatments can be attributed to a number of biological and technical issues. In this review, we detail the current limitations of immunotherapy target selection and design, and review computational methods to streamline therapy target discovery in a bioinformatics analysis pipeline. We describe specialized bioinformatics tools and databases for three main bottlenecks in immunotherapy target discovery: the cataloging of potentially antigenic proteins, the identification of potential HLA binders, and the selection epitopes and co-targets for single-epitope and multi-epitope strategies. We provide examples of application to the well-known tumor antigen HER2 and suggest bioinformatics methods to ameliorate therapy resistance and ensure efficient and lasting control of tumors.
2017-01-12T10:01:14Z
2017-01-12T10:01:14Z
2014-12-04
Article
Olsen, L. R., Campos, B., Barnkob, M. S., Winther, O., Brusic, V., & Andersen, M. H. (2014). Bioinformatics for cancer immunotherapy target discovery. Cancer Immunology, Immunotherapy, 63(12), 1235-1249. DOI: 10.1007/s00262-014-1627-7
http://nur.nu.edu.kz/handle/123456789/2257
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Cancer Immunology, Immunotherapy
oai:nur.nu.edu.kz:123456789/22582018-08-15T03:50:09Zcom_123456789_104com_123456789_67col_123456789_105
Pathway analysis and transcriptomics improve protein identification by shotgun proteomics from samples comprising small number of cells - a benchmarking study
Sun, Jing
Zhang, Guang Lan
Li, Siyang
Ivanov, Alexander R.
Fenyo, David
Lisacek, Frederique
Murthy, Shashi K.
Karger, Barry L.
Brusic, Vladimir
benchmarking
firearms
proteomics
Background: Proteomics research is enabled with the high-throughput technologies, but our ability to identify expressed proteome is limited in small samples. The coverage and consistency of proteome expression are critical problems in proteomics. Here, we propose pathway analysis and combination of microproteomics and transcriptomics analyses to improve mass-spectrometry protein identification from small size samples. Results: Multiple proteomics runs using MCF-7 cell line detected 4,957 expressed proteins. About 80% of expressed proteins were present in MCF-7 transcripts data; highly expressed transcripts are more likely to have expressed proteins. Approximately 1,000 proteins were detected in each run of the small sample proteomics. These proteins were mapped to gene symbols and compared with gene sets representing canonical pathways, more than 4,000 genes were extracted from the enriched gene sets. The identified canonical pathways were largely overlapping between individual runs. Of identified pathways 182 were shared between three individual small sample runs. Conclusions: Current technologies enable us to directly 10% of expressed proteomes from small sample comprising as few as 50 cells. We used knowledge-based approaches to elucidate the missing proteome that can be verified by targeted proteomics. This knowledge-based approach includes pathway analysis and combination of gene expression and protein expression data for target prioritization. Genes present in both the enriched gene sets (canonical pathways collection) and in small sample proteomics data correspond to approximately 50% of expressed proteomes in larger sample proteomics data. In addition, 90% of targets from canonical pathways were estimated to be expressed. The comparison of proteomics and transcriptomics data, suggests that highly expressed transcripts have high probability of protein expression. However, approximately 10% of expressed proteins could not be matched with the expressed transcripts.
2017-01-12T10:12:54Z
2017-01-12T10:12:54Z
2014-12-08
Article
Sun, J., Zhang, G. L., Li, S., Ivanov, A. R., Fenyo, D., Lisacek, F., ... Brusic, V. (2014). Pathway analysis and transcriptomics improve protein identification by shotgun proteomics from samples comprising small number of cells - a benchmarking study. BMC Genomics, 15, [S1]. DOI: 10.1186/1471-2164-15-S9-S1
http://nur.nu.edu.kz/handle/123456789/2258
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
BMC Genomics
oai:nur.nu.edu.kz:123456789/22592018-08-15T03:50:02Zcom_123456789_104com_123456789_67col_123456789_105
Therapeutic potential of electromagnetic fields for tissue engineering and wound healing
Saliev, T.
Mustapova, Z.
Kulsharova, G.
Bulanin, Denis
Mikhalovsky, S.
electromagnetic fields
tissue engineering
wound healing
Ability of electromagnetic fields (EMF) to stimulate cell proliferation and differentiation has attracted the attention of many laboratories specialized in regenerative medicine over the past number of decades. Recent studies have shed light on bio-effects induced by the EMF and how they might be harnessed to help control tissue regeneration and wound healing. Number of recent reports suggests that EMF has a positive impact at different stages of healing. Processes impacted by EMF include, but are not limited to, cell migration and proliferation, expression of growth factors, nitric oxide signalling, cytokine modulation, and more. These effects have been detected even during application of low frequencies (range: 30-300 kHz) and extremely low frequencies (range: 3-30 Hz). In this regard, special emphasis of this review is the applications of extremely low-frequency EMFs due to their bio-safety and therapeutic efficacy. The article also discusses combinatorial effect of EMF and mesenchymal stem cells for treatment of neurodegenerative diseases and bone tissue engineering. In addition, we discuss future perspectives of application of EMF for tissue engineering and use of metal nanoparticles activated by EMF for drug delivery and wound dressing.
2017-01-12T11:12:58Z
2017-01-12T11:12:58Z
2014-12-01
Article
Saliev, T., Mustapova, Z., Kulsharova, G., Bulanin, D., & Mikhalovsky, S. (2014). Therapeutic potential of electromagnetic fields for tissue engineering and wound healing. Cell Proliferation, 47(6), 485-493. DOI: 10.1111/cpr.12142
http://nur.nu.edu.kz/handle/123456789/2259
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Cell Proliferation
oai:nur.nu.edu.kz:123456789/22602018-08-15T03:50:09Zcom_123456789_104com_123456789_67col_123456789_105
Tumor antigens as proteogenomic biomarkers in invasive ductal carcinomas
Olsen, Lars Rønn
Campos, Benito
Winther, Ole
Sgroi, Dennis C.
Karger, Barry L.
Brusic, Vladimir
biomarker
cancer
gene expression
protein expression
protein-protein interaction
proteogenomics
tumor antigens
Background: The majority of genetic biomarkers for human cancers are defined by statistical screening of high-throughput genomics data. While a large number of genetic biomarkers have been proposed for diagnostic and prognostic applications, only a small number have been applied in the clinic. Similarly, the use of proteomics methods for the discovery of cancer biomarkers is increasing. The emerging field of proteogenomics seeks to enrich the value of genomics and proteomics approaches by studying the intersection of genomics and proteomics data. This task is challenging due to the complex nature of transcriptional and translation regulatory mechanisms and the disparities between genomic and proteomic data from the same samples. In this study, we have examined tumor antigens as potential biomarkers for breast cancer using genomics and proteomics data from previously reported laser capture microdissected ER+ tumor samples. Results: We applied proteogenomic analyses to study the genetic aberrations of 32 tumor antigens determined in the proteomic data. We found that tumor antigens that are aberrantly expressed at the genetic level and expressed at the protein level, are likely involved in perturbing pathways directly linked to the hallmarks of cancer. The results found by proteogenomic analysis of the 32 tumor antigens studied here, capture largely the same pathway irregularities as those elucidated from large-scale screening of genomics analyses, where several thousands of genes are often found to be perturbed. Conclusion: Tumor antigens are a group of proteins recognized by the cells of the immune system. Specifically, they are recognized in tumor cells where they are present in larger than usual amounts, or are physiochemically altered to a degree at which they no longer resemble native human proteins. This proteogenomic analysis of 32 tumor antigens suggests that tumor antigens have the potential to be highly specific biomarkers for different cancers.
2017-01-12T11:21:50Z
2017-01-12T11:21:50Z
2014-12-08
Article
Olsen, L. R., Campos, B., Winther, O., Sgroi, D. C., Karger, B. L., & Brusic, V. (2014). Tumor antigens as proteogenomic biomarkers in invasive ductal carcinomas. BMC Medical Genomics, 7(3), [523]. DOI: 10.1186/1755-8794-7-S3-S2
http://nur.nu.edu.kz/handle/123456789/2260
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
BMC Medical Genomics
oai:nur.nu.edu.kz:123456789/22612018-08-15T03:50:02Zcom_123456789_104com_123456789_67col_123456789_105
BlockLogo: visualization of peptide and sequence motif conservation
Olsen, Lars Rønn
Kudahl, Ulrich Johan
Simon, Christian
Sun, Jing
Schönbach, Christian
Reinherz, Ellis L.
Zhang, Guang Lan
Brusic, Vladimir
algorithms
Amino Acid Motifs
Amino Acid Sequence
Antigens, Plant
BlockLogo is a web-server application for the visualization of protein and nucleotide fragments, continuous protein sequence motifs, and discontinuous sequence motifs using calculation of block entropy from multiple sequence alignments. The user input consists of a multiple sequence alignment, selection of motif positions, type of sequence, and output format definition. The output has BlockLogo along with the sequence logo, and a table of motif frequencies. We deployed BlockLogo as an online application and have demonstrated its utility through examples that show visualization of T-cell epitopes and B-cell epitopes (both continuous and discontinuous). Our additional example shows a visualization and analysis of structural motifs that determine the specificity of peptide binding to HLA-DR molecules. The BlockLogo server also employs selected experimentally validated prediction algorithms to enable on-the-fly prediction of MHC binding affinity to 15 common HLA class I and class II alleles as well as visual analysis of discontinuous epitopes from multiple sequence alignments. It enables the visualization and analysis of structural and functional motifs that are usually described as regular expressions. It provides a compact view of discontinuous motifs composed of distant positions within biological sequences. BlockLogo is available at: http://research4.dfci.harvard.edu/cvc/blocklogo/ and http://met-hilab.bu.edu/blocklogo/.
2017-01-12T11:31:20Z
2017-01-12T11:31:20Z
2013-12-31
Article
Olsen, L. R., Kudahl, U. J., Simon, C., Sun, J., Schönbach, C., Reinherz, E. L., ... Brusic, V. (2013). BlockLogo: visualization of peptide and sequence motif conservation. Journal of Immunological Methods, 400-401, 37-44. DOI: 10.1016/j.jim.2013.08.014
http://nur.nu.edu.kz/handle/123456789/2261
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Journal of Immunological Methods
oai:nur.nu.edu.kz:123456789/22622018-08-15T03:50:09Zcom_123456789_104com_123456789_67col_123456789_105
The role of antioxidation and immunomodulation in postnatal multipotent stem cell-mediated cardiac repair
Saparov, Arman
Chen, Chien Wen
Beckman, Sarah A.
Wang, Yadong
Huard, Johnny
stem cells
repair
oxidative stress
tissue
blood vessels
cells
muscle
multipotent stem
Oxidative stress and inflammation play major roles in the pathogenesis of coronary heart disease including myocardial infarction (MI). The pathological progression following MI is very complex and involves a number of cell populations including cells localized within the heart, as well as cells recruited from the circulation and other tissues that participate in inflammatory and reparative processes. These cells, with their secretory factors, have pleiotropic effects that depend on the stage of inflammation and regeneration. Excessive inflammation leads to enlargement of the infarction site, pathological remodeling and eventually, heart dysfunction. Stem cell therapy represents a unique and innovative approach to ameliorate oxidative stress and inflammation caused by ischemic heart disease. Consequently, it is crucial to understand the crosstalk between stem cells and other cells involved in post-MI cardiac tissue repair, especially immune cells, in order to harness the beneficial effects of the immune response following MI and further improve stem cell-mediated cardiac regeneration. This paper reviews the recent findings on the role of antioxidation and immunomodulation in postnatal multipotent stem cell-mediated cardiac repair following ischemic heart disease, particularly acute MI and focuses specifically on mesenchymal, muscle and blood-vessel-derived stem cells due to their antioxidant and immunomodulatory properties.
2017-01-13T02:19:51Z
2017-01-13T02:19:51Z
2013
Article
Saparov, A., Chen, C. W., Beckman, S. A., Wang, Y., & Huard, J. (2013). The role of antioxidation and immunomodulation in postnatal multipotent stem cell-mediated cardiac repair. International Journal of Molecular Sciences, 14(8), 16258-16279.
http://nur.nu.edu.kz/handle/123456789/2262
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
International Journal of Molecular Sciences
oai:nur.nu.edu.kz:123456789/27492018-08-15T03:50:14Zcom_123456789_104com_123456789_67col_123456789_105
Dengue Virus NS1 Protein as a Diagnostic Marker: Commercially Available ELISA and Comparison to qRT-PCR and Serological Diagnostic Assays Currently Used by the State of Florida
Ambrose, Jason H.
Sekaran, Shamala Devi
Azizan, Azliyati
dengue Virus NS1 Protein
antibodies
DENV NS1 ELISA
DENV NS1
DENV
NS1 ELISA
The proper management of patients infected with dengue virus requires early detection. Here, real-time molecular assays have proven useful but have limitations, whereas ELISAs that detect antibodies are still favored but results are obtained too late to be of clinical value. The production of DENV NS1 peaks early during infection and its detection can combine the advantages of both diagnostic approaches. Methods. This study compared assays currently used for detecting DENV infection at the Florida Department of Health including anti-DENV IgM and IgG ELISAs as well as qRT-PCR, against a commercially available DENV NS1 ELISA. These comparisons weremade among a group of 21 human sera. Results. Nine of 14 (64.3%) DENV qRT-PCR+ samples were also DENV NS1+. Interestingly, the 5 NS1− samples that were qRT-PCR+ were additionally IgM− and IgG+ suggesting a
nonprimary infection. Compared to qRT-PCR, the NS1 assay had a sensitivity of 64.3%, specificity 100%, PPV of 100%, and NPV of 58.3%. Conclusions.The NS1 ELISA performed as expected in known DENV qRT-PCR+ samples; however negative NS1 results for qRT-PCR+ and IgG+ sera seemingly reduced the usefulness of the NS1 ELISA for nonprimary cases. We therefore conclude thatdiagnosis obtained via DENV NS1 ELISA deserves further investigation.
2017-11-08T04:44:27Z
2017-11-08T04:44:27Z
2017
Article
Ambrose, Jason H. et al.(>2), 2017, Dengue Virus NS1 Protein as a Diagnostic Marker: Commercially Available ELISA and Comparison to qRT-PCR and Serological Diagnostic Assays Currently Used by the State of Florida, Journal of Tropical Medicine, Vol. 2017, 6 pages
https://doi.org/10.1155/2017/8072491
http://nur.nu.edu.kz/handle/123456789/2749
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Open Access - the content is available to the general public
Attribution-NonCommercial-ShareAlike 3.0 United States
Journal of Tropical Medicine
oai:nur.nu.edu.kz:123456789/27472018-08-15T03:50:14Zcom_123456789_104com_123456789_67col_123456789_105
Stress-Induced Anxiety- and Depressive-Like Phenotype Associated with Transient Reduction in Neurogenesis in Adult Nestin- CreERT2/Diphtheria Toxin Fragment A Transgenic Mice
Yun, Sanghee
Donovan, Michael H.
Ross, Michele N.
Richardson, Devon R.
Reister, Robin
Farnbauch, Laure A.
Fischer, Stephanie J.
Riethmacher, Dieter
Gershenfeld, Howard K.
Lagace, Diane C.
Eisch, Amelia J.
hippocampal dysfunction
hippocampal dentate gyrus neurogenesis
neurogenesis
floxed diphtheria toxin fragment
Depression and anxiety involve hippocampal dysfunction, but the specific relationship
between these mood disorders and adult hippocampal dentate gyrus neurogenesis remains unclear. In both humans with MDD and rodent models of depression, administration of antidepressants increases DG progenitor and granule cell number, yet rodents with induced ablation of DG neurogenesis typically do not demonstrate depressive- or anxiety-like behaviors. The conflicting data may be explained by the varied duration and degree to which adult neurogenesis is reduced in different rodent neurogenesis ablation models. In order to test this hypothesis we examined how a transient–rather than permanent–inducible reduction in neurogenesis would alter depressive- and anxiety-like behaviors. Transgenic Nestin-CreERT2/floxed diphtheria toxin fragment A (DTA) mice (Cre+DTA+) and littermates (Cre+DTA-; control) were given tamoxifen (TAM) to induce recombination and decrease nestin-expressing stem cells and their progeny. The decreased neurogenesis was transient: 12 days post-TAM Cre+DTA+ mice had fewer DG proliferating Ki67+ cells and fewer DCX+ neuroblasts/immature neurons relative to control, but 30 days post-TAM Cre+DTA+mice had the same DCX+ cell number as control. This ability of DG neurogenesis to recover after partial ablation also correlated with changes in behavior. Relative to control, Cre+DTA+ mice tested between 12–30 days post-TAM displayed indices of a stress-induced anxiety phenotype–longer latency to consume highly palatable food in the unfamiliar cage in the novelty-induced hypophagia test, and a depression phenotype–longer time of immobility in the tail suspension test, but Cre+DTA+ mice tested after 30 days post-TAM did not. These findings suggest a functional association between adult neurogenesis and stress induced anxiety- and depressive-like behaviors, where induced reduction in DCX+ cells at the time of behavioral testing is coupled with stress-induced anxiety and a depressive phenotype, and recovery of DCX+ cell number corresponds to normalization of these behaviors.
2017-11-08T04:43:11Z
2017-11-08T04:43:11Z
2016-01-21
Article
Yun Sanghee et al.(>10), 2016, Stress-Induced Anxiety- and Depressive-Like Phenotype Associated with Transient Reduction in Neurogenesis in Adult Nestin- CreERT2/Diphtheria Toxin Fragment A Transgenic Mice, PLoS ONE,
doi:10.1371/journal.pone.0147256
http://nur.nu.edu.kz/handle/123456789/2747
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Open Access - the content is available to the general public
Attribution-NonCommercial-ShareAlike 3.0 United States
PLOS ONE
oai:nur.nu.edu.kz:123456789/27592018-08-15T03:50:14Zcom_123456789_104com_123456789_67col_123456789_105
Application of molecular genotyping to determine prevalence of HPV strains in Pap smears of Kazakhstan women
Niyazmetova, Luiza
Aimagambetova, Gulzhanat
Stambekova, Nazigul
Abugalieva, Zaurech
Seksembayeva, Korlukain
Syed, Ali
Azizan, Azliyati
HPV
CIN
cervical
cancer
prevalence
Kazakhstan
Objectives: Human papillomavirus is themain causative agent for cervical cancer. However, few data are available about HPV prevalence in Kazakhstan. The aims of this study were to genotype HPV DNA in Pap smear samples of women to determine prevalence of carcinogenic HPV types in Astana, Kazakhstan and to analyze the association between HPV positivity and the cytology results of patient samples.
Methods: Pap smear materials were obtained from 140 patients aged 18-59, who visited the outpatient gynecological clinic. Microscopic examination was done to detect dysplasia, and HPV genotyping was done using real-time multiplex PCR. Results: HPV testing showed that among 61 HPV positive patients, the most prevalent types were 16 and 18. Microscopic examination showed that 79% of the samples had normal cytology, while 13% had CIN grade I, 5% had CIN grade II, and 3% had CIN grade III. The analysis revealed that 12% of the samples had CIN cytology and presence of HPV. Approximately 31% had HPV without cervical dysplasia, while 8% of samples were CIN positive without HPV infection. A statistically significant relationship between HPV 16 and HPV 33 positive samples and CIN grade II and III was found. Conclusions: Overall, this study will help to strengthen and guide health policy implementation of primary and secondary cervical cancer prevention strategies in Kazakhstan.2016 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync nd/4.0/).
2017-11-08T10:57:13Z
2017-11-08T10:57:13Z
2016-11-20
Article
Niyazmetova Luiza et al.(>6), 2016(November 20), Application of molecular genotyping to determine prevalence of HPV strains in Pap smears of Kazakhstan women, International Journal of Infectious Diseases, 85-88pp
1201-9712
http://dx.doi.org/10.1016/j.ijid.2016.11.410
http://nur.nu.edu.kz/handle/123456789/2759
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Open Access - the content is available to the general public
Attribution-NonCommercial-ShareAlike 3.0 United States
International Journal of Infectious Diseases
oai:nur.nu.edu.kz:123456789/27702018-08-15T03:50:15Zcom_123456789_104com_123456789_67col_123456789_105
Cerebral palsy: a multidisciplinary, integrated approach is essential
Bulekbayeva, Sholpan
Daribayev, Zholtay
Ospanova, Sholpan
Vento, Sandro
cerebral palsy
2017-11-09T06:32:22Z
2017-11-09T06:32:22Z
2017-04
Article
Bulekbayeva Sholpan et al.(>3), 2017(April), Cerebral palsy: a multidisciplinary, integrated approach is essential, Elsevier
http://nur.nu.edu.kz/handle/123456789/2770
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Open Access - the content is available to the general public
Attribution-NonCommercial-ShareAlike 3.0 United States
Elsevier
oai:nur.nu.edu.kz:123456789/27712018-08-15T03:50:15Zcom_123456789_104com_123456789_67col_123456789_105
Macroporous Composite Cryogels with Embedded Polystyrene Divinylbenzene Microparticles for the Adsorption of Toxic Metabolites from Blood
Eichhorn, Tanja
Ivanov, Alexander E.
Dainiak, Maria B.
Leistner, André
Linsberger, Ingrid
Jungvid, Hans
Mikhalovsky, Sergey V.
Weber, Viktoria
monolithic adsorbents
PS-DVB
poly
blood
Composite monolithic adsorbents were prepared by the incorporation of neutral polystyrene divinylbenzene (PS-DVB) microparticles into macroporous polymer structures produced by cryogelation of agarose or poly(vinyl alcohol). The composite
materials exhibited excellent flow-through properties. Scanning electron microscopy of the composite cryogels revealed that the microparticles were covered by thin films of poly(vinyl alcohol) or agarose and thus were withheld in the monolith structure. Plain PS-DVB microparticles showed efficient adsorption of albumin-bound toxins related to liver failure (bilirubin and cholic acid) and of cytokines (tumor necrosis factor-alpha and interleukin-6). The rates of adsorption and the amount of adsorbed factors were lower for the embedded microparticles as compared to the parent PS-DVB microparticles, indicating the importance of the accessibility of the adsorbent pores. Still, the macroporous composite materials showed efficient adsorption of albuminbound
toxins related to liver failure as well as efficient binding of cytokines, combined with good blood compatibility. Thus, the incorporation of microparticles into macroporous polymer structures may provide an option for the development of adsorption modules for extracorporeal blood purification.
2017-11-09T06:32:41Z
2017-11-09T06:32:41Z
2013
Article
Eichhorn Tanja et al.(>7), 2013, Macroporous Composite Cryogels with Embedded Polystyrene Divinylbenzene Microparticles for the Adsorption of Toxic Metabolites from Blood, Journal of Chemistry, vol.2013, 8 pages
http://dx.doi.org/10.1155/2013/348412
http://nur.nu.edu.kz/handle/123456789/2771
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Open Access - the content is available to the general public
Attribution-NonCommercial-ShareAlike 3.0 United States
Journal of Chemistry
oai:nur.nu.edu.kz:123456789/28152018-08-15T03:50:18Zcom_123456789_104com_123456789_67col_123456789_105
Stress-Induced Anxiety- and Depressive-Like Phenotype Associated with Transient Reduction in Neurogenesis in Adult Nestin- CreERT2/Diphtheria Toxin Fragment A Transgenic Mice
Yun, Sanghee
Donovan, Michael H.
Ross, Michele N.
Richardson, Devon R.
Reister, Robin
Farnbauch, Laure A.
Fischer, Stephanie J.
Riethmacher, Dieter
Gershenfeld, Howard K.
Lagace, Diane C.
Eisch, Amelia J.
depression
anxiety
neurogenesis
MDD
transgenic
mice test
Research Subject Categories::MEDICINE
Depression and anxiety involve hippocampal dysfunction, but the specific relationship
between these mood disorders and adult hippocampal dentate gyrus neurogenesis remains unclear. In both humans with MDD and rodent models of depression, administration of antidepressants increases DG progenitor and granule cell number, yet rodents with induced ablation of DG neurogenesis typically do not demonstrate depressive- or anxiety-like behaviors. The conflicting data may be explained by the varied duration and degree to which adult neurogenesis is reduced in different rodent neurogenesis ablation models. In order to test this hypothesis we examined how a transient–rather than permanent–inducible reduction in neurogenesis would alter depressive- and anxiety-like behaviors. Transgenic Nestin-CreERT2/floxed diphtheria toxin fragment A (DTA) mice (Cre+DTA+) and littermates (Cre+DTA-; control) were given tamoxifen (TAM) to induce recombination and decrease nestin-expressing stem cells and their progeny. The decreased neurogenesis was transient: 12 days post-TAM Cre+DTA+ mice had fewer DG proliferating Ki67+ cells and fewer DCX+ neuroblasts/immature neurons relative to control, but 30 days post-TAM Cre+DTA+ mice had the same DCX+ cell number as control. This ability of DG neurogenesis to recover after partial ablation also correlated with changes in behavior. Relative to control, Cre+DTA+ mice tested between 12–30 days post-TAM displayed indices of a stress-induced anxiety phenotype–longer latency to consume highly palatable food in the unfamiliar cage in the novelty-induced hypophagia test, and a depression phenotype–longer time of immobility in
the tail suspension test, but Cre+DTA+ mice tested after 30 days post-TAM did not. These
findings suggest a functional association between adult neurogenesis and stress induced anxiety- and depressive-like behaviors, where induced reduction in DCX+ cells at the time
of behavioral testing is coupled with stress-induced anxiety and a depressive phenotype,
and recovery of DCX+ cell number corresponds to normalization of these behaviors.
2017-11-14T11:40:44Z
2017-11-14T11:40:44Z
2016-01-21
Article
Yun Sanghee et al(>10), 2016(January 21), Stress-Induced Anxiety- and Depressive-Like Phenotype Associated with Transient Reduction in Neurogenesis in Adult Nestin- CreERT2/Diphtheria Toxin Fragment A Transgenic Mice, Plos One,
doi:10.1371/journal.pone.0147256
http://nur.nu.edu.kz/handle/123456789/2815
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Open Access - the content is available to the general public
Attribution-NonCommercial-ShareAlike 3.0 United States
Plos One
oai:nur.nu.edu.kz:123456789/28552018-08-15T03:50:17Zcom_123456789_104com_123456789_67col_123456789_105
PHP127 Development of Hta In Kazakhstan
Bektur, C
HTA was introduced in Kazakhstan in 2009 as a result of an agreement of Kazakhstani Government with World Bank. As a consequence of this project, an HTA unit was created as part of Republican Centre of Healthcare Development(RCHD). There is lack of evidence on HTA concept in Kazakhstan, therefore, this research aims to gather information on the current state of development, processes and resources used in HTA in the country.
2017-12-12T11:07:11Z
2017-12-12T11:07:11Z
2016-11-01
Article
Bektur, C. (2016) PHP127 Development of Hta In Kazakhstan. Value in Health. Volume 19, Issue 7, Page A835
10983015
https://www.sciencedirect.com/science/article/pii/S1098301516311421
http://nur.nu.edu.kz/handle/123456789/2855
en
Value in Health
Copyright © 2016 Published by Elsevier Inc.
Open Access - the content is available to the general public
Value in Health
oai:nur.nu.edu.kz:123456789/28582018-08-15T03:50:17Zcom_123456789_104com_123456789_67col_123456789_105
Tuberculosis diagnosis using immunodominant, secreted antigens of Mycobacterium tuberculosis
Bekmurzayeva, Aliya
Sypabekova, Marzhan
Kanayeva, Damira
Tuberculosis diagnosis
Mpt64
Esat6
Cfp10
Ag85
Biosensor
Summary Tuberculosis (TB) remains a major public health concern in most low-income countries. Hence, rapid and sensitive TB diagnostics play an important role in detecting and preventing the disease. In addition to established diagnostic methods, several new approaches have been reported. Some techniques are simple but time-consuming, while others require complex instrumentation. One prominent and readily available approach is to detect proteins that Mycobacterium tuberculosis secretes, such as Mpt64, the 6-kDa early secreted antigenic target (Esat6), the 10-kDa culture filtrate protein (Cfp10), and the antigen 85 (Ag85) complex. Although their functions are not fully understood, a growing body of molecular evidence implicates them in M. tuberculosis virulence. Currently these biomarkers are either being used or investigated for use in skin patch tests, biosensor analyses, and immunochromatographic, immunohistochemical, polymerase chain reaction-based, and enzyme-linked immunosorbent assays. This review provides a comprehensive discussion of the roles these immunodominant antigens play in M. tuberculosis pathogenesis and compares diagnostic methods based on the detection of these proteins with more established tests for TB.
2017-12-13T04:19:34Z
2017-12-13T04:19:34Z
2013-07-01
Book chapter
Aliya Bekmurzayeva, Marzhan Sypabekova, Damira Kanayeva, Tuberculosis diagnosis using immunodominant, secreted antigens of Mycobacterium tuberculosis, In Tuberculosis, Volume 93, Issue 4, 2013, Pages 381-388
14729792
https://www.sciencedirect.com/science/article/pii/S1472979213000826
http://nur.nu.edu.kz/handle/123456789/2858
en
Tuberculosis
Copyright © 2013 Elsevier Ltd. All rights reserved.
Open Access - the content is available to the general public
Tuberculosis
oai:nur.nu.edu.kz:123456789/28602018-08-15T03:50:17Zcom_123456789_104com_123456789_67col_123456789_105
PMS37 Cost-Effectiveness Analysis of Tocilizumab Verse Infliximab for the Patients With Rheumatoid Arthritis in Kazakhstan
Bektur, C.
Kayir, F.
Nurgozhin, T.
The purpose of present study was to evaluate the cost-effectiveness of tocilizumab (TCZ) vs. infliximab (IFX) for the treatment of patients with rheumatoid arthritis (RA) from the perspective of Ministry of Health (MoH) over a patient lifetime horizon in Kazakhstan.
2017-12-13T04:46:26Z
2017-12-13T04:46:26Z
2014-11-01
Article
C. Bektur, F. Kayir, T. Nurgozhin, PMS37 - Cost-Effectiveness Analysis of Tocilizumab Verse Infliximab for the Patients With Rheumatoid Arthritis in Kazakhstan, In Value in Health, Volume 17, Issue 7, 2014, Page A379
10983015
https://www.sciencedirect.com/science/article/pii/S1098301514045562
http://nur.nu.edu.kz/handle/123456789/2860
en
Value in Health
Copyright © 2014 Published by Elsevier Inc.
Open Access - the content is available to the general public
Value in Health
oai:nur.nu.edu.kz:123456789/28612018-08-15T03:50:17Zcom_123456789_104com_123456789_67col_123456789_105
PGI28 Cost-Effectiveness of Simeprevir Vs. Telaprevir for The Triple Therapy of Hepatitis C In Kazakhstan
Bektur, C
Nurgozhin, T
Abdukhakimova, D
Hepatitis C Virus(HCV) is a growing health problem in the world. The aim of this study is to estimate a cost-effectiveness of a triple therapy(TT) with simeprevir compared to a TT with telaprevir for the previously treated with double therapy HCV patients in Kazakhstan.
2017-12-13T05:02:22Z
2017-12-13T05:02:22Z
2015-11-01
Article
C Bektur, T Nurgozhin, D Abdukhakimova, PGI28 - Cost-Effectiveness of Simeprevir Vs. Telaprevir for The Triple Therapy of Hepatitis C In Kazakhstan, In Value in Health, Volume 18, Issue 7, 2015, Page A627
10983015
https://www.sciencedirect.com/science/article/pii/S1098301515042850
http://nur.nu.edu.kz/handle/123456789/2861
en
Value in Health
Copyright © 2015 Published by Elsevier Inc.
Open Access - the content is available to the general public
Value in Health
oai:nur.nu.edu.kz:123456789/28622018-08-15T03:50:17Zcom_123456789_104com_123456789_67col_123456789_105
Effect of azelnidipine on beta-amyloid peptide induced dysfunction of cerebral endothelial cells in vitro
Askarova, Sholpan
Tsoy, Andrey
Teng, Tao
Lee, James
Zhumadilov, Zhaxybay
There is evidence that the receptor for advanced glycation endproducts (RAGE) is a binding site for amyloid β peptide (Aβ). RAGE is a multiligand cell surface receptor which is normally expressed in brain endothelium and, at low levels, in microglia and neurons. However, in Alzheimer's disease (AD) brains RAGE expression is increased by several-fold in cerebral endothelial cells (CECs), astrocytes, microglia, and neurons. Recent studies have demonstrated that RAGE is an important therapeutic target in AD. Therefore, there is a need to investigate new promising Aβ/RAGE axis blockers. It has been shown that azelnidipine (a highly lipid-soluble dihydropyridine-based calcium channel blocker) had a strong positive effect in RAGE-associated blood vessel damage in nondiabetic patients with stage I or II chronic kidney disease. In this study, we examined the effects of azelnidipine on A β 42 -induced activation of NADPH oxidase and consequent reactive oxygen species (ROS) generation, activation of NF-κB, ERK1/2 phosphorylation in CECs, and expression of P-selectin on the surface of the cells.
2017-12-13T05:14:51Z
2017-12-13T05:14:51Z
2014-07-01
Article
Sholpan Askarova, Andrey Tsoy, Tao Teng, James Lee, Zhaxybay Zhumadilov, Effect of azelnidipine on beta-amyloid peptide induced dysfunction of cerebral endothelial cells in vitro, In Alzheimer's & Dementia, Volume 10, Issue 4, Supplement, 2014, Pages P874-P875
15525260
https://www.sciencedirect.com/science/article/pii/S1552526014025114
http://nur.nu.edu.kz/handle/123456789/2862
en
Alzheimer's & Dementia
Copyright © 2014 Published by Elsevier B.V.
Open Access - the content is available to the general public
Alzheimer's & Dementia
oai:nur.nu.edu.kz:123456789/28632018-08-15T03:50:17Zcom_123456789_104com_123456789_67col_123456789_105
Role of ros in ab42-mediated activation of cerebral endothelial cells
Tsoy, Andrey
Shalakhmetova, Tamara
Umbayev, Bauyrzhan
Askarova, Sholpan
There is evidence that the deposition of amyloid-beta peptide (Aβ) in brain parenchyma and brain vessels is the main cause of neuronal dysfunction in Alzheimer's disease (AD). Aβ exhibits multiple cytotoxic effects to neurons and causes dysfunction of the blood brain barrier (BBB). In the AD brains an increased deposition of Aβ in the cerebral vasculature is correlated with increased transmigration of blood-born inflammatory cells and neurovascular inflammation. However, regulatory mediators of these processes remain to be elucidated. In this study, we examined a role of ROS in actin polymerization and expression of adhesion molecules on the surface of the cerebral endothelial cells (CECs) that are activated by Aβ42.
2017-12-13T05:21:50Z
2017-12-13T05:21:50Z
2014-07-01
Article
Andrey Tsoy, Tamara Shalakhmetova, Bauyrzhan Umbayev, Sholpan Askarova, ROLE OF ROS IN AB42-MEDIATED ACTIVATION OF CEREBRAL ENDOTHELIAL CELLS, In Alzheimer's & Dementia, Volume 10, Issue 4, Supplement, 2014, Page P875
15525260
https://www.sciencedirect.com/science/article/pii/S1552526014025126
http://nur.nu.edu.kz/handle/123456789/2863
en
Alzheimer's & Dementia
Copyright © 2014 Published by Elsevier B.V.
Open Access - the content is available to the general public
Alzheimer's & Dementia
oai:nur.nu.edu.kz:123456789/28642018-08-15T03:50:17Zcom_123456789_104com_123456789_67col_123456789_105
Cerebral palsy: a multidisciplinary, integrated approach is essential
Bulekbayeva, Sholpan
Daribayev, Zholtay
Ospanova, Sholpan
Vento, Sandro
Cerebral palsy, a syndrome of motor impairment resulting from a lesion in the developing brain, has a worldwide prevalence of 1·0–3·5 per 1000 livebirths.A life-course perspective needs to be adopted as more children live into their adolescence and adulthood. Individuals' participation in life and availability of family-centred services are very important and differ between countries. In low-income countries, most treatments are provided by families and multidisciplinary assessment is done in rural clinics.
2017-12-13T05:30:57Z
2017-12-13T05:30:57Z
2017-04-01
Article
Sholpan Bulekbayeva, Zholtay Daribayev, Sholpan Ospanova, Sandro Vento, Cerebral palsy: a multidisciplinary, integrated approach is essential, In The Lancet Global Health, Volume 5, Issue 4, 2017, Page e401
2214109X
https://www.sciencedirect.com/science/article/pii/S2214109X17300827
http://nur.nu.edu.kz/handle/123456789/2864
en
The Lancet Global Health
© 2017 The Author(s). Published by Elsevier Ltd.
Open Access - the content is available to the general public
The Lancet Global Health
oai:nur.nu.edu.kz:123456789/28652018-08-15T03:50:17Zcom_123456789_104com_123456789_67col_123456789_105
Developing a new medical school at a new university in Kazakhstan
McDonald, M.
Kanter, S.L.
Pignatelli, M.
Khamzina, N.
Mahoney, J.F.
Woodward, J.
Nazarbayev University (NU) was dedicated in June 2010 by Kazakhstan President Nursultan Nazarbayev, with the mission of making the republic's 15-year-old capital, Astana, Eurasia's leading research and educational center. Each NU academic unit is paired with an international partner; instruction is in English. The University of Pittsburgh School of Medicine (UPSOM) was selected as NU's partner to develop the NU School of Medicine (NUSOM) based on a U.S. model. Combined with the six hospitals of National Medical Holding (NMH), also part of NU, and NU's Center for Life Sciences, NU plans to create Kazakhstan's first integrated academic health system.
2017-12-13T08:28:21Z
2017-12-13T08:28:21Z
2014-05-01
Article
M. McDonald, S.L. Kanter, M. Pignatelli, N. Khamzina, J.F. Mahoney, J. Woodward, Developing a new medical school at a new university in Kazakhstan, In Annals of Global Health, Volume 80, Issue 3, 2014, Page 189
22149996
https://www.sciencedirect.com/science/article/pii/S2214999614001775
http://nur.nu.edu.kz/handle/123456789/2865
en
Annals of Global Health
Copyright © 2014 Published by Elsevier Inc.
Annals of Global Health
oai:nur.nu.edu.kz:123456789/28862018-08-15T03:50:17Zcom_123456789_104com_123456789_67col_123456789_105
CYP1A2 polymorphism does not modulate caffeine's effects on sprint performance or its metabolites during a soccer match simulation
Kingsley, M.
Devlin, B.
Belski, R.
Leveritt, M.
Chan, C.K.
The effectiveness of caffeine supplementation on exercise performance varies between individuals. Cytochrome P450 1A2 (CYP1A2) is an important enzyme that controls the primary catabolism of caffeine to paraxanthine, theobromine and theophylline, which are potent adenosine receptor antagonists. The cause of variation in responsiveness to caffeine might be associated with a (C/A) single nucleotide polymorphism of CYP1A2. This study assessed the influence of CYP1A2 polymorphism on caffeine metabolites and sprint performance during a soccer simulation protocol.
2017-12-14T04:47:43Z
2017-12-14T04:47:43Z
2017-01-01
Article
M. Kingsley, B. Devlin, R. Belski, M. Leveritt, C.K. Chan, CYP1A2 polymorphism does not modulate caffeine's effects on sprint performance or its metabolites during a soccer match simulation, In Journal of Science and Medicine in Sport, Volume 20, Supplement 1, 2017, Page e10
14402440
https://www.sciencedirect.com/science/article/pii/S1440244016302973
http://nur.nu.edu.kz/handle/123456789/2886
en
Journal of Science and Medicine in Sport
Copyright © 2016 Published by Elsevier Ltd.
Journal of Science and Medicine in Sport
oai:nur.nu.edu.kz:123456789/29012018-08-15T03:50:17Zcom_123456789_104com_123456789_67col_123456789_105
CDP-choline (ceraxon) treatment with hypoxia in the newborn infant
Issayeva, R.
Pushkarev, K.
2017-12-14T09:08:32Z
2017-12-14T09:08:32Z
2013-10-15
Article
R. Issayeva, K. Pushkarev, CDP-choline (ceraxon) treatment with hypoxia in the newborn infant, In Journal of the Neurological Sciences, Volume 333, Supplement 1, 2013, Page e561
0022510X
https://www.sciencedirect.com/science/article/pii/S0022510X13022776
http://nur.nu.edu.kz/handle/123456789/2901
en
Journal of the Neurological Sciences
Copyright © 2013 Published by Elsevier B.V.
Journal of the Neurological Sciences
oai:nur.nu.edu.kz:123456789/29032018-08-15T03:50:17Zcom_123456789_104com_123456789_67col_123456789_105
End-stage liver disease in eastern Europe and central Asia: action is needed
Vento, Sandro
Cainelli, Francesca
Ashimkhanova, Aiymkul
2017-12-14T09:18:23Z
2017-12-14T09:18:23Z
2016-09-01
Article
Sandro Vento, Francesca Cainelli, Aiymkul Ashimkhanova, End-stage liver disease in eastern Europe and central Asia: action is needed, In The Lancet Gastroenterology & Hepatology, Volume 1, Issue 1, 2016, Pages 8-9
24681253
https://www.sciencedirect.com/science/article/pii/S246812531630005X
http://nur.nu.edu.kz/handle/123456789/2903
en
The Lancet Gastroenterology & Hepatology
© 2016 Elsevier Ltd. All rights reserved.
The Lancet Gastroenterology & Hepatology
oai:nur.nu.edu.kz:123456789/29122018-08-15T03:50:18Zcom_123456789_104com_123456789_67col_123456789_105
Viral co-infections in high-risk communities of Pakistan
Shah, Adil A
Abidi, Syed H
Kalish, Marcia L
Vermund, Sten H
Ali, Syed
2017-12-15T03:24:52Z
2017-12-15T03:24:52Z
2015-04-01
Article
Adil A Shah, Syed H Abidi, Marcia L Kalish, Sten H Vermund, Syed Ali, Viral co-infections in high-risk communities of Pakistan, In The Lancet HIV, Volume 2, Issue 4, 2015, Pages e124-e125
23523018
https://www.sciencedirect.com/science/article/pii/S2352301815000399
http://nur.nu.edu.kz/handle/123456789/2912
en
The Lancet HIV
Copyright © 2015 Elsevier Ltd. All rights reserved.
The Lancet HIV
oai:nur.nu.edu.kz:123456789/29202018-08-15T03:50:19Zcom_123456789_104com_123456789_67col_123456789_105
Tuberculosis-immune reconstitution inflammatory syndrome
Lanzafame, Massimiliano
Vento, Sandro
Tuberculosis
IRIS
Immune reconstitution
HIV
Antiretrovirals
Abstract Tuberculosis-immune reconstitution inflammatory syndrome is an excessive immune response against Mycobacterium tuberculosis that may occur in either HIV-infected or uninfected patients, during or after completion of anti-TB therapy. In HIV-infected patients it occurs after initiation of antiretroviral therapy independently from an effective suppression of HIV viremia. There are two forms of IRIS: paradoxical or unmasking. Paradoxical IRIS is characterized by recurrent, new, or worsening symptoms of a treated case. Unmasking IRIS is an antiretroviral-associated inflammatory manifestation of a subclinical infection with a hastened presentation. The pathogenesis is incompletely understood and the epidemiology partially described. No specific tests can establish or rule out the diagnosis. Treatment is based on the use of anti-tuberculosis drugs sometime with adjunctive corticosteroids. Mortality is generally low.
2017-12-15T03:57:32Z
2017-12-15T03:57:32Z
2016-05-01
Article
Massimiliano Lanzafame, Sandro Vento, Tuberculosis-immune reconstitution inflammatory syndrome, In Journal of Clinical Tuberculosis and Other Mycobacterial Diseases, Volume 3, 2016, Pages 6-9
24055794
https://www.sciencedirect.com/science/article/pii/S2405579415300097
http://nur.nu.edu.kz/handle/123456789/2920
en
Journal of Clinical Tuberculosis and Other Mycobacterial Diseases
© 2016 Published by Elsevier Ltd.
Journal of Clinical Tuberculosis and Other Mycobacterial Diseases
oai:nur.nu.edu.kz:123456789/29272018-08-15T03:50:20Zcom_123456789_104com_123456789_67col_123456789_105
Febrile rhabdomyolysis of unknown origin in refugees coming from West Africa through the Mediterranean to Calabria, Italy
Vallone, Alfredo
Marino, Roberto
Vento, Sandro
2017-12-15T04:34:32Z
2017-12-15T04:34:32Z
2017-10-01
Article
Alfredo Vallone, Roberto Marino, Sandro Vento, Febrile rhabdomyolysis of unknown origin in refugees coming from West Africa through the Mediterranean to Calabria, Italy, In International Journal of Infectious Diseases, Volume 63, 2017, Pages 99-100
12019712
https://www.sciencedirect.com/science/article/pii/S120197121730214X
http://nur.nu.edu.kz/handle/123456789/2927
en
International Journal of Infectious Diseases
© 2017 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.
International Journal of Infectious Diseases
oai:nur.nu.edu.kz:123456789/29332018-08-15T03:50:18Zcom_123456789_104com_123456789_67col_123456789_105
Chapter 21 Biomedical Applications of Carbon Adsorbents
Mikhalovsky, Sergey V.
Sandeman, Susan R.
Howell, Carol A.
Phillips, Gary J.
Nikolaev, Vladimir G.
Activated carbon (AC) has a long history of use for medical applications due to its very high adsorption capacity and ability to adsorb a large range of substances. Recent advances in carbon materials science have produced a new generation of ACs with improved mechanical properties and porous structure, in a range of forms and with a breadth of applications in oral medicine, hemoperfusion devices, and wound care beyond that of traditional usage. The history and progressive development of AC for these applications are described including more recent developments in synthetic resin-based materials with optimized porosity to bind high molecular-weight and protein-bound biological toxins poorly removed by current systems. These highly optimized materials open up a range of treatment strategies with great potential to reduce healthcare cost burden and target improved acute and chronic disease treatment regimes.
2017-12-15T05:36:38Z
2017-12-15T05:36:38Z
2012-01-01
Article
Sergey V. Mikhalovsky, Susan R. Sandeman, Carol A. Howell, Gary J. Phillips and Vladimir G. Nikolaev, Chapter 21 - Biomedical Applications of Carbon Adsorbents, In Novel Carbon Adsorbents, edited by Juan M.D. Tascón,, Elsevier, Oxford, 2012, Pages 639-669
9780080977447
https://www.sciencedirect.com/science/article/pii/B9780080977447000211
http://nur.nu.edu.kz/handle/123456789/2933
en
Novel Carbon Adsorbents
Copyright © 2012 Elsevier Ltd. All rights reserved.
Novel Carbon Adsorbents
oai:nur.nu.edu.kz:123456789/29742018-08-15T03:50:20Zcom_123456789_104com_123456789_67col_123456789_105
Application of molecular genotyping to determine prevalence of HPV strains in Pap smears of Kazakhstan women
Niyazmetova, Luiza
Aimagambetova, Gulzhanat
Stambekova, Nazigul
Abugalieva, Zaurech
Seksembayeva, Korlukain
Ali, Syed
Azizan, Azliyati
HPV
CIN
cervical cancer
prevalence
Kazakhstan
Abstract ObjectivesHuman papillomavirus is the main causative agent for cervical cancer. However, few data are available about HPV prevalence in Kazakhstan. The aims of this study were to genotype HPV DNA in Pap smear samples of women to determine prevalence of carcinogenic HPV types in Astana, Kazakhstan and to analyze the association between HPV positivity and the cytology results of patient samples. MethodsPap smear materials were obtained from 140 patients aged 18-59, who visited the outpatient gynecological clinic. Microscopic examination was done to detect dysplasia, and HPV genotyping was done using real-time multiplex PCR. ResultsHPV testing showed that among 61 HPV positive patients, the most prevalent types were 16 and 18. Microscopic examination showed that 79% of the samples had normal cytology, while 13% had CIN grade I, 5% had CIN grade II, and 3% had CIN grade III. The analysis revealed that 12% of the samples had CIN cytology and presence of HPV. Approximately 31% had HPV without cervical dysplasia, while 8% of samples were CIN positive without HPV infection. A statistically significant relationship between HPV 16 and HPV 33 positive samples and CIN grade II and III was found. ConclusionsOverall, this study will help to strengthen and guide health policy implementation of primary and secondary cervical cancer prevention strategies in Kazakhstan.
2017-12-20T09:57:21Z
2017-12-20T09:57:21Z
2017-01-01
Article
Luiza Niyazmetova, Gulzhanat Aimagambetova, Nazigul Stambekova, Zaurech Abugalieva, Korlukain Seksembayeva, Syed Ali, Azliyati Azizan, Application of molecular genotyping to determine prevalence of HPV strains in Pap smears of Kazakhstan women, In International Journal of Infectious Diseases, Volume 54, 2017, Pages 85-88
12019712
https://www.sciencedirect.com/science/article/pii/S1201971216316289
http://nur.nu.edu.kz/handle/123456789/2974
en
International Journal of Infectious Diseases
© 2016 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.
International Journal of Infectious Diseases
oai:nur.nu.edu.kz:123456789/29762018-08-15T03:50:19Zcom_123456789_104com_123456789_67col_123456789_105
Food authentication: state of the art and prospects
Danezis, Georgios P
Tsagkaris, Aristidis S
Brusic, Vladimir
Georgiou, Constantinos A
Food authentication is analytical process that validates label information about the food origin and production process. This review discusses emerging food authenticity indicators including rare earth elements, metabolic profiles, sensory profile evaluation, and microbial fingerprinting. A scientometric evaluation that highlights research trends and emerging approaches of this evolving field is presented. We analyzed and provided an insight into most commonly studied food types and drinks and highlighted the most promising authenticity markers for each major group. Finally, we discussed the data explosion that is emerging and discussed the developing trends in the field. The future of food authentication lies in Big Data since we are already collecting and analyzing huge amounts of data that converge from multiple sources. Big Data Analytics will include standardization of experiments, study design workflows, and of data reporting. Emerging data analytical tools will include integration of geographic information systems with climate information systems and metabolomics databases for precise spatio-temporal identification of food origin.
2017-12-20T10:04:46Z
2017-12-20T10:04:46Z
2016-08-01
Article
Georgios P Danezis, Aristidis S Tsagkaris, Vladimir Brusic, Constantinos A Georgiou, Food authentication: state of the art and prospects, In Current Opinion in Food Science, Volume 10, 2016, Pages 22-31
22147993
https://www.sciencedirect.com/science/article/pii/S2214799316300844
http://nur.nu.edu.kz/handle/123456789/2976
en
Current Opinion in Food Science
© 2016 Elsevier Ltd. All rights reserved.
Current Opinion in Food Science
oai:nur.nu.edu.kz:123456789/30472019-10-17T05:50:13Zcom_123456789_104com_123456789_67col_123456789_105
Relación presión/volumen en el laboratorio de ecocardiografía de estrés. ¿Cómo influye el tamaño del ventrículo izquierdo (dimensión diastólica del ventrículo izquierdo)?
Bombardini, Tonino
Mulieri, Louis A.
Salvadori, Stefano
Costantino, Marco Fabio
Scali, Maria Chiara
Marzilli, Mario
Picano, Eugenio
Ecocardiografía de estrés
Fisiología
Diástole
Contractilidad
Resumen Introducción y objetivosLa variación en la relación presión/volumen telesistólica entre el reposo y el estrés máximo es un índice de contractilidad del ventrículo izquierdo independiente de la poscarga. Aún no está claro si depende del volumen telediastólico y hasta qué punto. El objetivo de este estudio es evaluar la dependencia de la variación de la relación presión/volumen telesistólica entre el reposo y estrés (Δ) y el volumen telediastólico en pacientes con ecocardiografía de estrés negativa y con todos los intervalos de función ventricular izquierda en reposo. MétodosAnalizamos los datos interpretables obtenidos de 891 pacientes (593 varones; 63 ± 12 años) con fracción de eyección del 47 ± 12%: 338 pacientes estaban sanos, prácticamente sanos o hipertensos; 229 tenían arteriopatía coronaria y 324, miocardiopatía dilatada isquémica o no isquémica. Se los estudió con ecocardiografía de estrés en ejercicio (n =172), dipiridamol (n = 482) o dobutamina (n = 237). La relación presión/volumen telesistólica se evaluó en reposo y en estrés máximo a partir de una medición bruta de la presión arterial sistólica mediante esfigmógrafo con manguito y el volumen telesistólico, por ecocardiografía bidimensional mediante el método de Simpson biplanar. ResultadosLos valores absolutos de la variación reposo-estrés en la relación presión/volumen telesistólica fueron más altos con ejercicio y dobutamina que con dipiridamol. En la población general, se observó relación inversa entre la relación presión/volumen telesistólica y el volumen telediastólico en reposo (r2 = 0,69; p < 0,001) y en estrés máximo (r2 = 0,56; p < 0,001), pero no se observó esta relación al considerar la variación reposo-estrés de la relación presión/volumen telesistólica (r2 = 0,13). ConclusionesEl volumen telediastólico ventricular izquierdo no afecta a la variación reposo-estrés de la relación presión/volumen telesistólica en ventrículos izquierdos normales o anómalos durante el estrés físico o farmacológico.
2017-12-22T08:04:39Z
2017-12-22T08:04:39Z
2017-02-01
Article
Tonino Bombardini, Louis A. Mulieri, Stefano Salvadori, Marco Fabio Costantino, Maria Chiara Scali, Mario Marzilli, Eugenio Picano, Relación presión/volumen en el laboratorio de ecocardiografía de estrés. ¿Cómo influye el tamaño del ventrículo izquierdo (dimensión diastólica del ventrículo izquierdo)?, In Revista Española de Cardiología, Volume 70, Issue 2, 2017, Pages 96-104
03008932
https://www.sciencedirect.com/science/article/pii/S030089321630207X
http://nur.nu.edu.kz/handle/123456789/3047
Revista Española de Cardiología
© 2016 Sociedad Española de Cardiología. Published by Elsevier España, S.L.U. All rights reserved.
oai:nur.nu.edu.kz:123456789/30482018-08-15T03:50:22Zcom_123456789_104com_123456789_67col_123456789_105
Pressure-volume Relationship in the Stress-echocardiography Laboratory: Does (Left Ventricular End-diastolic) Size Matter?
Bombardini, Tonino
Mulieri, Louis A.
Salvadori, Stefano
Costantino, Marco Fabio
Scali, Maria Chiara
Marzilli, Mario
Picano, Eugenio
Stress echocardiography
Physiology
Diastole
Contractility
Abstract Introduction and objectivesThe variation between rest and peak stress end-systolic pressure-volume relation is an afterload-independent index of left ventricular contractility. Whether and to what extent it depends on end-diastolic volume remains unclear. The aim of this study was to assess the dependence of the delta rest-stress end-systolic pressure-volume relation on end-diastolic volume in patients with negative stress echo and all ranges of resting left ventricular function. MethodsWe analyzed interpretable data obtained in 891 patients (593 men, age 63 ± 12 years) with ejection fraction 47% ± 12%: 338 were normal or near-normal or hypertensive; 229 patients had coronary artery disease; and 324 patients had ischemic or nonischemic dilated cardiomyopathy. They were studied with exercise (n = 172), dipyridamole (n = 482) or dobutamine (n = 237) stress echocardiography. The end-systolic pressure-volume relation was evaluated at rest and peak stress from raw measurement of systolic arterial pressure by cuff sphygmomanometer and end-systolic volume by biplane Simpson rule 2-dimensional echocardiography. ResultsAbsolute values of delta rest-stress end-systolic pressure-volume relation were higher for exercise and dobutamine than for dipyridamole. In the overall population, an inverse relationship between end-systolic pressure-volume relation and end-diastolic volume was present at rest (r2 = 0.69, P < .001) and peak stress (r2 = 0.56, P < .001), but was absent if the delta rest-stress end-systolic pressure-volume relation was considered (r2 = 0.13). ConclusionsLeft ventricular end-diastolic volume does not affect the rest-stress changes in end-systolic pressure-volume relation in either normal or abnormal left ventricles during physical or pharmacological stress.
2017-12-22T08:08:01Z
2017-12-22T08:08:01Z
2017-02-01
Article
Tonino Bombardini, Louis A. Mulieri, Stefano Salvadori, Marco Fabio Costantino, Maria Chiara Scali, Mario Marzilli, Eugenio Picano, Pressure-volume Relationship in the Stress-echocardiography Laboratory: Does (Left Ventricular End-diastolic) Size Matter?, In Revista Española de Cardiología (English Edition), Volume 70, Issue 2, 2017, Pages 96-104
18855857
https://www.sciencedirect.com/science/article/pii/S1885585716301384
http://nur.nu.edu.kz/handle/123456789/3048
en
Revista Española de Cardiología (English Edition)
© 2016 Sociedad Española de Cardiología. Published by Elsevier España, S.L.U. All rights reserved.
Revista Española de Cardiología (English Edition)
oai:nur.nu.edu.kz:123456789/31122018-08-15T03:50:24Zcom_123456789_104com_123456789_67col_123456789_105
Reallocation of Olfactory Cajal-Retzius Cells Shapes Neocortex Architecture
de Frutos, Cristina A.
Bouvier, Guy
Arai, Yoko
Thion, Morgane S.
Lokmane, Ludmilla
Keita, Maryama
Garcia-Dominguez, Mario
Charnay, Patrick
Hirata, Tatsumi
Riethmacher, Dieter
Grove, Elizabeth A.
Tissir, Fadel
Casado, Mariano
Pierani, Alessandra
Garel, Sonia
Cajal-Retzius cells
neocortex
olfactory cortex
marginal zone
layer 1
migration
NMDA receptors
interneurons
E/I ratio
Summary The neocortex undergoes extensive developmental growth, but how its architecture adapts to expansion remains largely unknown. Here, we investigated how early born Cajal-Retzius (CR) neurons, which regulate the assembly of cortical circuits, maintain a dense superficial distribution in the growing neocortex. We found that CR cell density is sustained by an activity-dependent importation of olfactory CR cells, which migrate into the neocortex after they have acted as axonal guidepost cells in the olfactory system. Furthermore, using mouse genetics, we showed that CR cell density severely affects the architecture of layer 1, a key site of input integration for neocortical networks, leading to an excitation/inhibition ratio imbalance. Our study reveals that neurons reenter migration several days after their initial positioning, thereby performing sequential developmental roles in olfactory cortex and neocortex. This atypical process is essential to regulate CR cell density during growth, which in turn ensures the correct wiring of neocortical circuitry. Video Abstract
2018-01-05T03:52:30Z
2018-01-05T03:52:30Z
2016-10-19
Article
Cristina A. de Frutos, Guy Bouvier, Yoko Arai, Morgane S. Thion, Ludmilla Lokmane, Maryama Keita, Mario Garcia-Dominguez, Patrick Charnay, Tatsumi Hirata, Dieter Riethmacher, Elizabeth A. Grove, Fadel Tissir, Mariano Casado, Alessandra Pierani, Sonia Garel, Reallocation of Olfactory Cajal-Retzius Cells Shapes Neocortex Architecture, In Neuron, Volume 92, Issue 2, 2016, Pages 435-448
08966273
https://www.sciencedirect.com/science/article/pii/S0896627316305797
http://nur.nu.edu.kz/handle/123456789/3112
en
Neuron
© 2016 Elsevier Inc.
Neuron
oai:nur.nu.edu.kz:123456789/33382018-08-15T03:50:29Zcom_123456789_104com_123456789_67col_123456789_105
Epidemiology of HPV Infection and HPV-Related Cancers in Kazakhstan: a Review
Aimagambetova, Gulzhanat
Azizan, Azliyati
Human papillomavirus (HPV)
cervical cancer
HPV epidemiology
related cancers
HPV vaccination
Background: Cancer is one of the most prevalent causes of mortality worldwide. In the cervix it is considered to be caused by different high-risk human papillomavirus (HPV) types. Although many studies have already been conducted worldwide on the epidemiology of HPV infection and their oncogenic properties, limited data are available on HPV prevalence, incidence and genotype specific dissemination in Kazakhstan. Methods: To review the distribution of HPV infection, electronic databases (e.g. PubMed, Web of Science and Google Scholar) were searched for peer reviewed articles in English. The study was performed during June-July 2017 with a review of 39 relevant articles, published up to July 31, 2017. The following inclusion criteria were applied: general population data, cytology results available, and use of polymerase chain reaction (PCR) and/or Hybrid Capture® 2, Digene Corp., USA for HPV detection. Results:
As reported in limited studies, the prevalence of HPV infection in Kazakhstan ranges from 43.8% to 55.8%. However, the scenario with regard to epidemiology of HPV related cancers in Kazakhstan is not very clear. One study reported a decline of laryngeal cancer observed during the recent years, whereas cervical cancer incidence has increased to about 3000 new cervical cancer cases, and about 1,000 cervical cancer deaths each year. Conclusion: The high incidence of cervical cancer with a significant mortality rate in Kazakhstan is evidence of HPV infection abundance despite an absence of HPV screening and low public awareness of the problem. Having a well-informed understanding of the role of HPV infection could enhance the public’s acceptance of screening and intervention programs to reduce morbidity and mortality in the country due to HPV infection. Thus, the purpose of this review article is to summarize the existing
data, identifying directions for future research on HPV epidemiology and HPV-related diseases in Kazakhstan.
2018-06-14T05:47:01Z
2018-06-14T05:47:01Z
2018-04-03
Article
Aimagambetova, Gulzhanat. Azizan, Azliyati. (2018) Epidemiology of HPV Infection and HPV-Related Cancers in Kazakhstan: a Review. Asian Pacific Journal of Cancer Prevention. Vol 19
http://nur.nu.edu.kz/handle/123456789/3338
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
About Asian Pacific Journal of Cancer Prevention
oai:nur.nu.edu.kz:123456789/33392018-08-15T03:50:16Zcom_123456789_104com_123456789_67col_123456789_105
An observational case study of hospital associated infections in a critical care unit in Astana, Kazakhstan
Viderman, Dmitriy
Khamzina, Yekaterina
Kaligozhin, Zhannur
Khudaibergenova, Makhira
Zhumadilov, Agzam
Intensive care unit (ICU)
Hospital associated infections (HAI)
Surgical site infections (SSI)
Ventilator associated pneumonia (VAP)
Blood stream infections (BSI)
Urinary tract infections (UTI)
Background: Hospital Associated infections (HAI) are very common in Intensive Care Units (ICU) and are usually associated with use of invasive devices in the patients. This study was conducted to determine the prevalence and etiological agents of HAI in a Surgical ICU in Kazakhstan, and to assess the impact of these infections on ICU stay
and mortality. Objective: To assess the rate of device-associated infections and causative HAI etiological agents in an ICU at the National Research Center for Oncology and Transplantation (NRCOT) in Astana, Kazakhstan. Methods: This retrospective, observational study was conducted in a 12-bed ICU at the NRCOT, Astana, Kazakhstan.
We enrolled all patients who were admitted to the ICU from January, 2014 through November 2015, aged 18 to 90 years of age who developed an HAI. Results: The most common type of HAI was surgical site infection (SSI), followed by ventilator-associated
pneumonia (VAP), catheter-related blood stream infection (BSI) and catheter-associated urinary tract infection (UTI). The most common HAI was SSI with Pseudomonas aeruginosa as the most common etiological agent. The second most common HAI was VAP also with P. aeruginosa followed by BSI which was also associated
with P. aeruginosa (in 2014) and Enterococcus faecalis, and Klebsiella pneumoniae (in 2015) as the most common etiological agents causing these infections.
2018-06-14T06:06:40Z
2018-06-14T06:06:40Z
2018
Article
Viderman, Dmitriy et al. (2018) An observational case study of hospital associated infections in a critical care unit in Astana, Kazakhstan. Antimicrobial Resistance and Infection Control
https://doi.org/10.1186/s13756-018-0350-0
http://nur.nu.edu.kz/handle/123456789/3339
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Antimicrobial Resistance and Infection Control
oai:nur.nu.edu.kz:123456789/35222018-09-21T21:00:48Zcom_123456789_104com_123456789_67col_123456789_105
First year medical students’ learning style preferences and their correlation with performance in different subjects within the medical course
Hernández-Torrano, Daniel
Syed, Ali
Chan, Chee-Kai
Learning styles
Academic performance
First-year
Medical education
Medical subject
Background: Students commencing their medical training arrive with different educational backgrounds and a diverse range of learning experiences. Consequently, students would have developed preferred approaches to acquiring and processing information or learning style preferences. Understanding first-year students’ learning style preferences is important to success in learning. However, little is understood about how learning styles
impact learning and performance across different subjects within the medical curriculum. Greater understanding of the relationship between students’ learning style preferences and academic performance in specific medical subjects would be valuable. Methods: This cross-sectional study examined the learning style preferences of first-year medical students and how they differ across gender. This research also analyzed the effect of learning styles on academic performance across different subjects within a medical education program in a Central Asian university. A total of 52 students (57.7% females) from two batches of first-year medical school completed the Index of Learning Styles Questionnaire, which measures four dimensions of learning styles: sensing-intuitive; visual-verbal; active-reflective; sequential-global. Results: First-year medical students reported preferences for visual (80.8%) and sequential (60.5%) learning styles, suggesting that these students preferred to learn through demonstrations and diagrams and in a linear and sequential way. Our results indicate that male medical students have higher preference for visual learning style over verbal, while females seemed to have a higher preference for sequential learning style over global. Significant associations were found between sensing-intuitive learning styles and performance in Genetics
[β = −0.46, B = −0.44, p < 0.01] and Anatomy [β = −0.41, B = −0.61, p < 0.05] and between sequential-global styles and performance in Genetics [β = 0.36, B = 0.43, p < 0.05]. More specifically, sensing learners were more likely to perform better than intuitive learners in the two subjects and global learners were more likely to perform better than sequential learners in Genetics. Conclusion: This knowledge will be helpful to individual students to improve their performance in these subjects by adopting new sensing learning techniques. Instructors can also benefit by modifying and adapting more appropriate teaching approaches in these subjects. Future studies to validate this observation will be valuable.
2018-09-21T09:44:05Z
2018-09-21T09:44:05Z
2017-08-08
Article
Hernández-Torrano et al. First year medical students’ learning style preferences and their correlation with performance in different subjects within the medical course. BMC Medical Education. (2017)
http://nur.nu.edu.kz/handle/123456789/3522
https://doi.org/10.1186/s12909-017-0965-5
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
BMC Medical Education
oai:nur.nu.edu.kz:123456789/35392018-10-16T21:00:31Zcom_123456789_104com_123456789_67col_123456789_105
Defensive medicine: It is time to finally slow down an epidemic
Vento, Sandro
Cainelli, Francesca
Vallone, Alfredo
Adverse event
Clinical reasoning
Defensive medicine
Doctor-patient relationship
Healthcare cost
Medical education
Medical error
Defensive medicine is widespread and practiced the
world over, with serious consequences for patients,
doctors, and healthcare costs. Even students and residents
are exposed to defensive medicine practices and
taught to take malpractice liability into consideration
when making clinical decisions. Defensive medicine is
generally thought to stem from physicians’ perception
that they can easily be sued by patients or their relatives
who seek compensation for presumed medical errors.
However, in our view the growth of defensive medicine
should be seen in the context of larger changes in the
conception of medicine that have taken place in the
last few decades, undermining the patient–physician
trust, which has traditionally been the main source of
professional satisfaction for physicians. These changes
include the following: time directly spent with patients
has been overtaken by time devoted to electronic health
records and desk work; family doctors have played a
progressively less central role; clinical reasoning is being
replaced by guidelines and algorithms; the public at large
and a number of young physicians tend to believe that
medicine is a perfect science rather than an imperfect
art, as it continues to be; and modern societies do not
tolerate the inevitable morbidity and mortality. To finally
reduce the increasing defensive behavior of doctors
around the world, the decriminalization of medical errors
and the assurance that they can be dealt with in civil
courts or by medical organizations in all countries could
help but it would not suffice. Physicians and surgeons
should be allowed to spend the time they need with their
patients and should give clinical reasoning the importance
it deserves. The institutions should support the doctors
who have experienced adverse patient events, and the
media should stop reporting with excessive evidence
presumed medical errors and subject physicians to “public
trials” before they are eventually judged in court.
2018-10-16T06:01:45Z
2018-10-16T06:01:45Z
2018-10-06
Article
Vento, Sandro; Cainelli, Francesca; Vallone, Alfredo. (2018) Defensive medicine: It is time to finally slow down an epidemic. World Journal of Clinical Cases
https://dx.doi.org/10.12998/wjcc.v6.i11.000
http://nur.nu.edu.kz/handle/123456789/3539
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
World Journal of Clinical Cases
oai:nur.nu.edu.kz:123456789/35402018-10-17T21:00:34Zcom_123456789_104com_123456789_67col_123456789_105
Liver cirrhosis in sub-Saharan Africa: neglected, yet important
Vento, Sandro
Dzudzor, Bartholomew
Cainelli, Francesca
liver cirrhosis
HBV
HCV
Clinical efforts and research on liver diseases have been
scarce in sub-Saharan Africa. The first Conference on
Liver Disease in Africa (Nairobi, Sept 13–15, 2018),
gathering all stakeholders from the continent, is a
welcome step towards greater attention to the problem,
and the important issue of liver cirrhosis.
2018-10-17T03:17:10Z
2018-10-17T03:17:10Z
2018-09-12
Article
Vento, Sandro; Dzudzor, Bartholomew; Cainelli, Francesca. (2018) Liver cirrhosis in sub-Saharan Africa: neglected, yet important. Lancet Glob Health
http://dx.doi.org/10.1016/ S2214-109X(18)30344-9
http://nur.nu.edu.kz/handle/123456789/3540
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Lancet Glob Health
oai:nur.nu.edu.kz:123456789/35502018-10-22T21:00:40Zcom_123456789_104com_123456789_67col_123456789_105
Значение автоматизированной антенатальной кардиотокографии для оценки функионального состояния плодов, имеющих задержку развития
Курмангали, Ж. К.
Укыбасова, Т. М.
Бапаева, Г. Б.
Аймагамбетова, Г. Н.
беременные высокого риска перинатальной патологии
задержка внутриутробного развития плода
автоматизированная антенатальная кардиотокография
вариабельность базального ритма частоты сердечных сокращений плода
нарушение функционального состояния плода
Применение в акушерской
практике метода автоматизированной
антенатальной кардиотокографии
позволяет устранить субъективизм
и повысить надежность оценки
состояния плода в группе беременных
высокого акушерского и перинатального
риска. Установлено, что в группе
беременных высокого риска
нарушения состояния плода при
кардиотокографическом динамическом
наблюдении выявляются уже во
II триместре. При этом наиболее
ранними и достоверными показателями
нарушения состояния плода являются:
снижение вариабельности базального
ритма частоты сердечных сокращений
плода, отсутствие эпизодов высокой
вариабельности или превалирование
эпизодов низкой вариабельности
над высокими, значение показателя
STV — короткая вариабельность менее
4,0. Полученные данные позволяют
рекомендовать применение метода
автоматизированной антенатальной
кардиотокографии в группе беременных
высокого перинатального риска
для ранней диагностики нарушения
функционального состояния плода.
Возможность оценить с высокой
надежностью степень тяжести
метаболического ацидоза плода
позволит своевременно решить вопросы
оптимальной акушерской тактики
ведения беременности, срока и метода
родоразрешения у данной категории
беременных.
2018-10-22T09:00:34Z
2018-10-22T09:00:34Z
2012-04
Article
Курмангали, Ж. К., Укыбасова, Т. М., Бапаева, Г. Б., Аймагамбетова, Г. Н.. (2012) Значение автоматизированной антенатальной кардиотокографии для оценки функионального состояния плодов, имеющих задержку развития. Журнал акушерства и женских болезней.
1684-0461
http://nur.nu.edu.kz/handle/123456789/3550
ru
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Журнал акушерства и женских болезней
oai:nur.nu.edu.kz:123456789/36192018-11-16T21:00:50Zcom_123456789_104com_123456789_67col_123456789_105
Mammalian HP1 Isoforms Have Specific Roles in Heterochromatin Structure and Organization
Bosch-Presegue, Laia
Raurell-Vila, Helena
Thackray, Joshua K.
Singh, Prim B.
Serrano, Lourdes
Vaquero, Alejandro
Mammalian HP1 Isoforms
Heterochromatin Structure
HP1 is a structural component of heterochromatin.
Mammalian HP1 isoforms HP1a, HP1b, and HP1g
play different roles in genome stability, but their precise
role in heterochromatin structure is unclear.
Analysis of Hp1a
/ , Hp1b
/ , and Hp1g
/ MEFs
show that HP1 proteins have both redundant and
unique functions within pericentric heterochromatin
(PCH) and also act globally throughout the genome.
HP1a confines H4K20me3 and H3K27me3 to regions
within PCH, while its absence results in a global
hyper-compaction of chromatin associated with a
specific pattern of mitotic defects. In contrast,
HP1b is functionally associated with Suv4-20h2 and
H4K20me3, and its loss induces global chromatin
decompaction and an abnormal enrichment of
CTCF in PCH and other genomic regions. Our work
provides insight into the roles of HP1 proteins in heterochromatin
structure and genome stability.
2018-11-16T08:36:55Z
2018-11-16T08:36:55Z
2017
Article
Laia Bosch-Presegue´ , Helena Raurell-Vila, Joshua K. Thackray, Prim B. Singh, Lourdes Serrano, Alejandro Vaquero. Mammalian HP1 Isoforms Have Specific Roles in Heterochromatin Structure and Organization. 2017. Cell Reports
http://nur.nu.edu.kz/handle/123456789/3619
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Cell Reports
oai:nur.nu.edu.kz:123456789/37932023-11-11T14:45:15Zcom_123456789_104com_123456789_67col_123456789_105
Molecular epidemiology of HIV-1 subtype A in former Soviet Union countries
Aibekova, Lazzat
Foley, Brian
Hortelano, Gonzalo
Raees, Muhammad
Abdraimov, Sabit
Toichuev, Rakhmanbek
Ali, Syed
Sandstrom, Paul
While in other parts of the world it is on decline, incidence of HIV infection continues to rise
in the former Soviet Union (FSU) countries. The present study was conducted to investigate
the patterns and modes of HIV transmission in FSU countries. We performed phylogenetic
analysis of publicly available 2705 HIV-1 subtype A pol sequences from thirteen FSU countries:
Armenia, Azerbaijan, Belarus, Estonia, Georgia, Kazakhstan, Kyrgyzstan, Latvia, Lithuania,
Moldova, Russia, Ukraine and Uzbekistan. Our analysis showed that the clusters
from FSU countries were intermixed, indicating a possible role of transmigration in HIV
transmission. Injection drug use was found to be the most frequent mode of transmission,
while the clusters from PWID and heterosexual transmission were intermixed, indicating
bridging of HIV infection across populations. To control the expanding HIV epidemic in this
region, harm reduction strategies should be focused on three modes of transmission,
namely, cross-border migration, injection drug use and heterosexual.
2019-03-26T06:50:23Z
2019-03-26T06:50:23Z
2018-02-01
Article
Aibekova L, Foley B, Hortelano G, Raees M, Abdraimov S, Toichuev R, et al. (2018) Molecular epidemiology of HIV-1 subtype A in former Soviet Union countries. PLoS ONE 13(2): e0191891. https://doi.org/10.1371/journal. pone.0191891
http://nur.nu.edu.kz/handle/123456789/3793
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
PLoS ONE
oai:nur.nu.edu.kz:123456789/37942019-03-28T21:00:28Zcom_123456789_104com_123456789_67col_123456789_105
Opium trade and the spread of HIV in the Golden Crescent
Farooq, Syeda Ayesha
Rasooly, Mohammad Hafiz
Abidi, Syed Hani
Modjarrad, Kayvon
Ali, Syed
HIV
Opium
Golden crescent
Afghanistan
Iran
Pakistan
The Golden Crescent region of South Asia—comprising Afghanistan, Iran, and Pakistan—is a principal global site for opium production and distribution. Over the past few decades, war, terrorism, and a shifting political landscape have facilitated an active heroin trade throughout the region. Protracted conflict has exacerbated already dire socio-economic conditions and political strife within the region and contributed to a consequent rise in opiate trafficking and addiction among the region’s inhabitants. The worsening epidemic of injection drug use has paralleled the rising incidence of HIV and other blood-borne infections in the region and drawn attention to the broader implications of the growing opiate trade in the Golden Crescent. The first step in addressing drug use is to recognize that it is not a character flaw but a form of mental illness, hence warranting humane
treatment of drug users. It is also recommended that the governments of the Golden Crescent countries encourage substitution of opium with licit crops and raise awareness among the general public about the perils of opium use.
2019-03-28T04:20:29Z
2019-03-28T04:20:29Z
2017
Article
Farooq, Syeda Ayesha et al. (2017) Opium trade and the spread of HIV in the Golden Crescent. Harm Reduction Journal
http://dx.doi.org/10.1186/s12954-017-0170-1
http://nur.nu.edu.kz/handle/123456789/3794
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Harm Reduction Journal
oai:nur.nu.edu.kz:123456789/37952019-03-28T21:00:22Zcom_123456789_104com_123456789_67col_123456789_105
HIV-1 Subtype A Gag Variability and Epitope Evolution
Abidi, Syed Hani
Kalish, Marcia L.
Abbas, Farhat
Rowland-Jones, Sarah
Ali, Syed
Objective: The aim of this study was to examine the course of time-dependent evolution of HIV-1 subtype A on a global
level, especially with respect to the dynamics of immunogenic HIV gag epitopes.
Methods: We used a total of 1,893 HIV-1 subtype A gag sequences representing a timeline from 1985 through 2010, and 19
different countries in Africa, Europe and Asia. The phylogenetic relationship of subtype A gag and its epidemic dynamics
was analysed through a Maximum Likelihood tree and Bayesian Skyline plot, genomic variability was measured in terms of
GRA substitutions and Shannon entropy, and the time-dependent evolution of HIV subtype A gag epitopes was examined.
Finally, to confirm observations on globally reported HIV subtype A sequences, we analysed the gag epitope data from our
Kenyan, Pakistani, and Afghan cohorts, where both cohort-specific gene epitope variability and HLA restriction profiles of
gag epitopes were examined.
Results: The most recent common ancestor of the HIV subtype A epidemic was estimated to be 195661. A period of
exponential growth began about 1980 and lasted for approximately 7 years, stabilized for 15 years, declined for 2–3 years,
then stabilized again from about 2004. During the course of evolution, a gradual increase in genomic variability was
observed that peaked in 2005–2010. We observed that the number of point mutations and novel epitopes in gag also
peaked concurrently during 2005–2010.
Conclusion: It appears that as the HIV subtype A epidemic spread globally, changing population immunogenetic pressures
may have played a role in steering immune-evolution of this subtype in new directions. This trend is apparent in the
genomic variability and epitope diversity of HIV-1 subtype A gag sequences.
2019-03-28T04:28:49Z
2019-03-28T04:28:49Z
2014-06
Article
Abidi, Syed Hani et al. (2014) HIV-1 Subtype A Gag Variability and Epitope Evolution. Public Library of Science
http://dx.doi.org/10.1371/journal.pone.0093415
http://nur.nu.edu.kz/handle/123456789/3795
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Public Library of Science
oai:nur.nu.edu.kz:123456789/37962019-03-28T21:00:29Zcom_123456789_104com_123456789_67col_123456789_105
Burden of Communicable Disease among the Native and Repatriating Afghans
Abidi, Syed H
Ali, Farwa
Shah, Farida
Abbas, Farhat
Ali, Syed
Owing to the capricious political and
economic conditions of Afghanistan during the past few decades, the burden of
communicable disease, especially bloodborne and sexually transmitted infections,
has been steadily mounting in this population. Afghan refugees who sought asylum
and settled in foreign countries encountered many a barrier to standard health
care, which led to the establishment of
several epidemics in the refugee population as well. In recent years, repatriation of
these refugees back to their homeland has
raised new concerns about control and
prevention of potential epidemics in the
returning and indigenous Afghan populations. Mixing of existing infections, as well
as of risk behaviors and practices, in the
two populations, if left unattended, can
lead to unforeseen, uncontrollable outcomes.
2019-03-28T06:35:20Z
2019-03-28T06:35:20Z
2012-10
Article
Abidi, Syed H et al. (2012) Burden of Communicable Disease among the Native and Repatriating Afghans. Public Library of Science
15537366
http://dx.doi.org/10.1371/journal.ppat.1002926
http://nur.nu.edu.kz/handle/123456789/3796
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Public Library of Science
oai:nur.nu.edu.kz:123456789/37972023-11-11T14:45:16Zcom_123456789_104com_123456789_67col_123456789_105
The Spread of HIV in Pakistan: Bridging of the Epidemic between Populations
Khanani, Muhammad R.
Somani, Mehreen
Rehmani, Sadiq S.
Veras, Nazle M. C.
Ali, Syed
Thorne, Claire
In the last two decades, ‘concentrated epidemics’ of human immunodeficiency virus (HIV) have established in several high risk groups in Pakistan, including Injecting Drug Users (IDUs) and among men who have sex with men (MSM). To explore the transmission patterns of HIV infection in these major high-risk groups of Pakistan, 76 HIV samples were analyzed from MSM, their female spouses and children, along with 26 samples from a previously studied cohort of IDUs. Phylogenetic analysis of HIV gag gene sequences obtained from these samples indicated a substantial degree of intermixing between the IDU and MSM populations, suggesting a bridging of HIV infection from IDUs, via MSM, to the MSM spouses and children. HIV epidemic in Pakistan is now spreading to the female spouses and offspring of bisexual MSM. HIV control and awareness programs must be refocused to include IDUs, MSM, as well as bisexual MSM, and their spouses and children.
2019-03-28T06:47:36Z
2019-03-28T06:47:36Z
2011-07-25
Article
: Khanani MR, Somani M, Rehmani SS, Veras NMC, Salemi M, et al. (2011) The Spread of HIV in Pakistan: Bridging of the Epidemic between Populations. PLoS ONE 6(7): e22449. doi:10.1371/journal.pone.0022449
19326203
http://dx.doi.org/10.1371/journal.pone.0022449
http://nur.nu.edu.kz/handle/123456789/3797
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Public Library of Science
oai:nur.nu.edu.kz:123456789/38462019-04-25T21:00:49Zcom_123456789_104com_123456789_67col_123456789_105
Pediatric Celiac Disease in Central and East Asia: Current Knowledge and Prevalence
Poddighe, Dimitri
Rakhimzhanova, Marzhan
Marchenko, Yelena
Catassi, Carlo
celiac disease
children
HLA-DQ
prevalence
Asia
The current prevalence of pediatric Celiac Disease (CD) is estimated to be around 1% in the
general population, worldwide. However, according to the geographic area, a great variability of
CD prevalence has been described. Whereas a number of studies are available from Europe, North
and South America, Australia, South-West Asia, and North Africa, the knowledge and awareness of
CD in large parts of the remaining world areas is definitively poor. In several countries of Central
and East Asia, the consumption of wheat is consistent and/or has significantly increased in recent
decades, and CD is supposed to be underdiagnosed in children. In this mini-review, we aimed to
summarize the current knowledge about the prevalence of pediatric CD in Central and East Asia,
paying attention to the HLA-DQ immunogenetic background as well. Indeed, CD is likely not to be
as uncommon as previously or currently thought in countries like Russia, Kazakhstan, and China, in
addition to India, where pediatric CD has been clearly showed to be quite prevalent. Therefore, there
is an urgent need for population-based studies on the prevalence of CD in those countries, especially
in children, in order to increase the awareness of this disease and to improve the diagnostic strategy
in these areas.
2019-04-25T06:51:06Z
2019-04-25T06:51:06Z
2019-01-12
Article
http://dx.doi.org/10.3390/medicina55010011
http://nur.nu.edu.kz/handle/123456789/3846
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
MDPI
oai:nur.nu.edu.kz:123456789/38592019-04-25T21:00:30Zcom_123456789_104com_123456789_67col_123456789_105
L Chromosome Behaviour and Chromosomal Imprinting in Sciara Coprophila
Singh, Prim B.
Belyakin, Stepan
supernumerary chromosomes
heterochromatin
parent-of-origin effects
paternal X chromosome
maternal X chromosome
controlling element
The retention of supernumerary chromosomes in the germ-line of Sciara coprophila is
part of a highly-intricate pattern of chromosome behaviours that have fascinated cytogeneticists
for over 80 years. Germ-line limited (termed L or “limited”) chromosomes are cytologically
heterochromatic and late-replicating, with more recent studies confirming they possess epigenetic
hallmarks characteristic of constitutive heterochromatin. Little is known about their genetic
constitution although they have been found to undergo cycles of condensation and de-condensation at
different stages of development. Unlike most supernumeraries, the L chromosomes in S. coprophila are
thought to be indispensable, although in two closely related species Sciara ocellaris and Sciara reynoldsi
the L chromosomes, have been lost during evolution. Here, we review what we know about L
chromosomes in Sciara coprophila. We end by discussing how study of the L chromosome condensation
cycle has provided insight into the site and timing of both the erasure of parental “imprints” and also
the placement of a putative “imprint” that might be carried by the sperm into the egg.
2019-04-25T10:30:19Z
2019-04-25T10:30:19Z
2018-09-03
Article
http://dx.doi.org/10.3390/genes9090440
http://nur.nu.edu.kz/handle/123456789/3859
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
oai:nur.nu.edu.kz:123456789/42762019-12-05T18:45:23Zcom_123456789_104com_123456789_67col_123456789_105
Assessment of total mercury content in water of the Balkyldak Lake-reservoir, Pavlodar, Kazakhstan
Yapiev, Vadim
Inglezakis, V.J.
Korobeinyk, A.V.
Azat, S.
Research Subject Categories::MEDICINE
This study aimed at determining the levels of total mercury in water samples
collected in the vicinity of the Lake-reservoir Balkyldak in Pavlodar, Kazakhstan. Legacy of
the chloralkali process in Pavlodar which involved the mercury cell method while producing a
chlorine-free sodium hydroxide leads to serious environmental problems. At the time of the
unit continuous operation in between 1971-1993 approximately 1000 metric tons of metallic
mercury has been released into the wastewater holding pond at the chemical plant. For many
years, mercury collected in the wastewater pond was a subject of the Kazakhstan sharply
continental climate fluctuation and overflow to the Balkyldak Lake-reservoir, creating a
significant mercury contamination issue for the whole region. Mercury emitted from industrial
eluents in environment carries predominantly anthropogenic pollutants. Due to its natural cycle
through atmosphere, water and soil in different forms prompts the widespread global mercury
pollution. In present investigation, samples collected in a close proximity of the Balkyldak
Lake were analyzed in respect to the geological location of collection.
2019-11-01T05:38:44Z
2019-11-01T05:38:44Z
2019-02-21
Article
A V Korobeinyk et al 2018 IOP Conf. Ser.: Earth Environ. Sci. 167 012006
0.1088/1755-1315/167/1/012006
http://nur.nu.edu.kz/handle/123456789/4276
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
IOP Publishing
oai:nur.nu.edu.kz:123456789/43562019-12-11T21:02:45Zcom_123456789_104com_123456789_67col_123456789_105
ZEB1 and IL-6/11-STAT3 signalling cooperate to define invasive potential of pancreatic cancer cells via differential regulation of the expression of S100 proteins
Al-Ismaeel, Qais
Neal, Christopher P.
Al-Mahmoodi, Hanaa
Almutairi, Zamzam
Al-Shamarti, Ibtihal
Straatman, Kees
Jaunbocus, Nabil
Irvine, Andrew
Issa, Eyad
Moreman, Catherine
Dennison, Ashley R.
Sayan, A. Emre
McDearmid, Jonathan
Greaves, Peter
Tulchinsky, Eugene
Kriajevska, Marina
pancreatic ductal adenocarcinoma
PDAC
ZEB1
IL-6/11-STAT3
pancreatic cancer cells
S100 proteins
https://www.nature.com/articles/s41416-019-0483-9#Abs1
Background
S100 proteins have been implicated in various aspects of cancer, including epithelial-mesenchymal transitions (EMT), invasion and metastasis, and also in inflammatory disorders. Here we examined the impact of individual members of this family on the invasion of pancreatic ductal adenocarcinoma (PDAC) cells, and their regulation by EMT and inflammation.
Methods
Invasion of PDAC cells was analysed in zebrafish embryo xenografts and in transwell invasion assays. Expression and regulation of S100 proteins was studied in vitro by immunoblotting, quantitative PCR and immunofluorescence, and in pancreatic lesions by immunohistochemistry.
Results
Whereas the expression of most S100 proteins is characteristic for epithelial PDAC cell lines, S100A4 and S100A6 are strongly expressed in mesenchymal cells and upregulated by ZEB1. S100A4/A6 and epithelial protein S100A14 respectively promote and represses cell invasion. IL-6/11-STAT3 pathway stimulates expression of most S100 proteins. ZEB1 synergises with IL-6/11-STAT3 to upregulate S100A4/A6, but nullifies the effect of inflammation on S100A14 expression.
Conclusion
EMT/ZEB1 and IL-6/11-STAT3 signalling act independently and congregate to establish the expression pattern of S100 proteins, which drives invasion. Although ZEB1 regulates expression of S100 family members, these effects are masked by IL-6/11-STAT3 signalling, and S100 proteins cannot be considered as bona fide EMT markers in PDAC.
2019-12-11T05:46:01Z
2019-12-11T05:46:01Z
2019-07-02
Article
Al-Ismaeel, Q., Neal, C. P., Al-Mahmoodi, H., Almutairi, Z., Al-Shamarti, I., Straatman, K., … Kriajevska, M. (2019). ZEB1 and IL-6/11-STAT3 signalling cooperate to define invasive potential of pancreatic cancer cells via differential regulation of the expression of S100 proteins. British Journal of Cancer, 121(1), 65–75. https://doi.org/10.1038/s41416-019-0483-9
000473525700009
http://nur.nu.edu.kz/handle/123456789/4356
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
NATURE PUBLISHING GROUP
oai:nur.nu.edu.kz:123456789/43572019-12-11T21:02:13Zcom_123456789_104com_123456789_67col_123456789_105
Public knowledge, attitude and practice toward diabetes mellitus in Hail region, Saudi Arabia
Aljofan, Mohamad
Altebainawi, Ali
Alrashidi, Mubarak Nsha
knowledge
attitude
practice
diabetes mellitus
general population
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6643049/
Background: There is a rapid increase in the number of people with type II diabetes worldwide with many preventable cases. Diabetes control or prevention is partially influenced by the individual’s knowledge, attitude and practice toward the disease.
Objective: The aim of this study was to determine the general public knowledge, attitude and practice toward diabetes in the region of Hail, which is located northern of the Saudi capital.
Methodology: This is a cross-sectional study conducted during the period of March 2018 until September 2018. We randomly selected participants over the age of 18 years at different locations.
Results: A total of 738 participants (428 females and 310 males) with and an age range of 18–61 years and mean age of 35±12 were surveyed. The majority of the participants, regardless of education levels, residence and employment status showed high knowledge of diabetes (82%) and knew that physical activity can positively affect/prevent diabetes. Participant's age was found to have a significant association with participant's knowledge of diabetes with the ≥35 year old group knew the different types of diabetes (p<0.001) and knew that high carbohydrate consumption can increase the risk of diabetes (p<0.001), but only the younger group <18 year old's identified diabetes as a genetic disorder (p<0.001).
Conclusion: The high knowledge of the disease and its risk factors were not truly mirrored in the participant’s attitude and practice toward the disease. Therefore, it is quite essential for the local health authorities to implement awareness programs to improve public attitude and practice toward diabetes.
2019-12-11T05:46:03Z
2019-12-11T05:46:03Z
2019-07-16
Article
Aljofan, M., Altebainawi, A., & Alqaaboubi Alrashidi, M. N. (2019). Public knowledge, attitude and practice toward diabetes mellitus in Hail region, Saudi Arabia. International Journal of General Medicine, Volume 12, 255–262. https://doi.org/10.2147/ijgm.s214441
000477634800001
http://nur.nu.edu.kz/handle/123456789/4357
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
DOVE MEDICAL PRESS LTD
oai:nur.nu.edu.kz:123456789/43582019-12-11T21:01:56Zcom_123456789_104com_123456789_67col_123456789_105
Metformin: A stroke of luck
Aljofan, Mohamad
Gaipov, Abduzhappar
metformin
PCOS
cancer
cardiovascular
mechanism of action
http://www.ejgm.co.uk/Metformin-A-stroke-of-luck,108679,0,2.html
Metformin is an oral glucose-lowering drug that has been widely used for more than half a century. Both the American Diabetes Association and the European Association for the study of Diabetes recommend metformin as the first line therapy for the treatment of type II diabetes mellitus. Metformin has considerably evolved from being an antidiabetic into a drug with a wider potential of therapeutic uses such as anticancer, antiaging, treatment of gestational hypertension, preeclampsia and weight loss. In addition to the in-label use of metformin as an anti-hyperglycemic medication, it is widely prescribed as an off label therapy for young women with Poly Cystic Ovary Syndrome for the treatment of symptoms associated with the disorder. This article is a brief summary that lists and briefly discusses current and potential non-hyperglycemic uses and effects of metformin.
2019-12-11T05:46:05Z
2019-12-11T05:46:05Z
2019-03
Article
Aljofan, M., & Gaipov, A. (2019). Metformin: A stroke of luck. Electronic Journal of General Medicine, 16(3). https://doi.org/10.29333/ejgm/108679
000475757600007
http://nur.nu.edu.kz/handle/123456789/4358
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
MODESTUM LTD
oai:nur.nu.edu.kz:123456789/43592019-12-11T21:02:49Zcom_123456789_104com_123456789_67col_123456789_105
Anticancer activity of metformin: a systematic review of the literature
Aljofan, Mohamad
Riethmacher, Dieter
AMPK
anticancer
cancer
mechanism of action
metformin
https://www.ncbi.nlm.nih.gov/pubmed/31534778
BACKGROUND:
The anticancer activity of metformin has been confirmed against several cancer types in vitro and in vivo. However, the underlying mechanisms of metformin in the treatment of cancer are not fully understood. This systematic review aims to discuss the possible anticancer mechanism of action of metformin.
METHOD:
A search through different databases was conducted, including Medline and EMBASE.
RESULTS:
A total of 96 articles were identified of which 56 were removed for duplication and 24 were excluded after reviewing the title and abstract. A total of 12 research articles were included that describe different antiproliferative mechanisms that may contribute to the antineoplastic effects of metformin.
CONCLUSION:
This analysis discussed the potential anticancer activity of metformin and highlighted the importance of AMPK as a potential target for anticancer therapy.
2019-12-11T05:46:07Z
2019-12-11T05:46:07Z
2019-08
Article
Aljofan, M., & Riethmacher, D. (2019). Anticancer activity of metformin: a systematic review of the literature. Future Science OA, 5(8), FSO410. https://doi.org/10.2144/fsoa-2019-0053
000490580000002
http://nur.nu.edu.kz/handle/123456789/4359
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
FUTURE SCI LTD
oai:nur.nu.edu.kz:123456789/43612019-12-11T21:03:11Zcom_123456789_104com_123456789_67col_123456789_105
Influence of workplace incivility on the quality of nursing care
Alshehry, Abdualrahman Saeed
Alquwez, Nahed
Almazan, Joseph
Namis, Ibrahim Mohammed
Cruz, Jonas Preposi
incivility
nurses
nursing care
quality of nursing care
Saudi Arabia
https://onlinelibrary.wiley.com/doi/epdf/10.1111/jocn.15051
Aims and objectives
To examine the influence of workplace incivility on the quality of nursing care.
Background
Recent evidence describes workplace incivility as a serious concern in the healthcare setting worldwide. Exposure to workplace incivility can alter a nurse's behaviour, thought process and perspective towards the nursing profession. However, there is insufficient evidence to determine whether workplace incivility might be associated with the quality of nursing care in Saudi Arabia.
Design
A quantitative and cross‐sectional study.
Method
A survey was carried out amongst 378 nurses in two government hospitals in Saudi Arabia from February 2018–May 2018 using the Nurse Incivility and quality of nursing care scales. Multivariate multiple regression was performed to investigate the influence of the uncivil experiences of nurses from different sources on the different aspects of quality of nursing care. The study adhered to STROBE guideline (see Appendix S1).
Results
The overall mean of the quality of nursing care scale was 3.14 (SD = 0.66) from a scale of 1–5, with patient satisfaction receiving the highest mean dimension (mean = 3.27, SD = 0.72) and health promotion the lowest mean dimension (mean = 3.08, SD = 0.74). Experience in the present hospital and the hospital were associated with the overall quality of nursing care. General and nurse incivility exerted a multivariate effect on overall quality of nursing care and its different dimensions.
Conclusion
General incivility and nurse incivility were found to negatively impact quality of nursing care and its different dimensions.
Relevance to clinical practice
Stronger policies geared towards eliminating workplace incivility should be implemented as uncivil acts can lead to poor quality of nursing care. Nurse administrators and nurses should be pro‐active in recognising, preventing, approaching, reporting and intervening with uncivil acts in the hospital to protect these workers from these types of behaviours and avoid their negative impacts on patient care.
2019-12-11T05:59:04Z
2019-12-11T05:59:04Z
2019-09
Article
Alshehry, A. S., Alquwez, N., Almazan, J., Namis, I. M., & Cruz, J. P. (2019). Influence of workplace incivility on the quality of nursing care. Journal of Clinical Nursing, 28(23–24), 4582–4594. https://doi.org/10.1111/jocn.15051
10.1111/jocn.15051
http://nur.nu.edu.kz/handle/123456789/4361
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
WILEY
oai:nur.nu.edu.kz:123456789/43732020-07-14T04:31:27Zcom_123456789_104com_123456789_67col_123456789_105
Khat-related liver disease in sub-Saharan Africa: neglected, yet important reply
Vento, Sandro
Dzudzor, Bartholomew
Cainelli, Francesca
Tachi, Kenneth
The question of khat's liver toxicity is complex. It contains numerous compounds, including alkaloids, terpenoids, flavonoids, sterols, glycosides, tannins, amino acids, vitamins, and minerals.5 Research into the deleterious effects of khat has largely concentrated on the pharmacological effects of cathinone and cathine on the cardiovascular system and CNS, and studies on possible liver toxicity in humans are restricted to Ethiopia and Somaliland and to reports from western Europe almost exclusively involving Somali men. Additional research is necessary to establish the prevalence of liver toxicity in people who chew khat in Ethiopia versus Djibouti (where liver toxicity is apparently not observed)6 and Yemen (which has the highest proportion of users worldwide),7 and in Kenya, Uganda, and Madagascar, where studies have not been done. Also necessary is investigating the mechanisms and progression of liver damage induced by khat, the effects of gender (khat toxicity is observed much more frequently in males)8 and genetics (genetic variants in CYP2D6 might play a role in liver toxicity),9 and the contribution of other compounds found in khat and of contaminating herbicides and pesticides to liver disease.10
Current evidence shows that hepatitis viruses and alcohol are the main contributors to the incidence of liver cirrhosis throughout sub-Saharan Africa1 and efforts should continue to curb their effects.
We declare no competing interests.
2019-12-11T08:32:15Z
2019-12-11T08:32:15Z
2019-03
Article
Vento, S., Dzudzor, B., Cainelli, F., & Tachi, K. (2019). Khat-related liver disease in sub-Saharan Africa: neglected, yet important–Authors' reply. The Lancet Global Health, 7(3), e311.
https://www.thelancet.com/journals/langlo/article/PIIS2214-109X(18)30521-7/fulltext
http://nur.nu.edu.kz/handle/123456789/4373
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Nazarbayev University School of Medicine
oai:nur.nu.edu.kz:123456789/43742019-12-11T21:00:51Zcom_123456789_104com_123456789_67col_123456789_105
AllergoOncology: Microbiota in allergy and cancer-A European Academy for Allergy and Clinical Immunology position paper
Vangelista, Luca
Untersmayr, Eva
Bergmann, Christoph
Bianchini, Rodolfo
Cozen, Wendy
Gould, Hannah
Hartmann, Karin
Josephs, Debra
Levi-Schaffer, Francesca
Penichet, Manuel L.
O'Mahony, Liam
Poli, Aurelie
Redegeld, Frank A
Roth-Walter, Franziska
Turner, Michelle C.
Karagiannis, Sophia N
Jensen-Jarolim, Erika
The microbiota can play important roles in the development of human immunity and the establishment of immune homeostasis. Lifestyle factors including diet, hygiene, and exposure to viruses or bacteria, and medical interventions with antibiotics or anti-ulcer medications, regulate phylogenetic variability and the quality of cross talk between innate and adaptive immune cells via mucosal and skin epithelia. More recently, microbiota and their composition have been linked to protective effects for health. Imbalance, however, has been linked to immune-related diseases such as allergy and cancer, characterized by impaired, or exaggerated immune tolerance, respectively. In this AllergoOncology position paper, we focus on the increasing evidence defining the microbiota composition as a key determinant of immunity and immune tolerance, linked to the risk for the development of allergic and malignant diseases. We discuss novel insights into the role of microbiota in disease and patient responses to treatments in cancer and in allergy. These may highlight opportunities to improve patient outcomes with medical interventions supported through a restored microbiome.
2019-12-11T08:43:32Z
2019-12-11T08:43:32Z
2019-01-12
Article
Untersmayr, E., Bax, H. J., Bergmann, C., Bianchini, R., Cozen, W., Gould, H. J., ... & O'Mahony, L. (2019). AllergoOncology: Microbiota in allergy and cancer—A European Academy for Allergy and Clinical Immunology position paper. Allergy, 74(6), 1037-1051.
https://onlinelibrary.wiley.com/doi/full/10.1111/all.13718
http://nur.nu.edu.kz/handle/123456789/4374
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
oai:nur.nu.edu.kz:123456789/43982019-12-12T21:01:17Zcom_123456789_104com_123456789_67col_123456789_105
Relevance of HLA-DQB1*02 Allele in the Genetic Predisposition of Children with Celiac Disease: Additional Cues from a Meta-Analysis
Capittini, Cristina
De Silvestri, Annalisa
Rebuffi, Chiara
Tinelli, Carmine
Poddighe, Dimitri
celiac disease
children
HLA-DQB1*02
screening
first-degree relatives
https://www.ncbi.nlm.nih.gov/pubmed/31121940
Background and Objectives: Celiac disease (CD) is a multifactorial immune-mediated disorder, triggered by the ingestion of gluten in genetically-predisposed subjects carrying MHC-DQ2 and -DQ8 heterodimers, which are encoded by four HLA-DQ allelic variants, overall. This meta-analysis aims at providing further epidemiological support to the predominant relevance of one specific allele, namely HLA-DQB1*02, in the predisposition and genetic risk of CD. Materials and Methods: We performed a search of MEDLINE/PubMed, Embase, Web of Science, and Scopus, retrieving all publications (case-control study, cross-sectional, and retrospective cohort study) on the association between HLA class II polymorphisms and first-degree relatives (FDRs) of children with CD. After a critical reading of the articles, two investigators independently performed data extraction according to the following inclusion criteria: HLA class II genes, any DQ and DR molecules, and CD diagnosed following the current clinical guidelines. A third participant was consulted for discussion to reach an agreement concerning discrepancies. Results: Our search strategy selected 14 studies as being eligible for inclusion, and those were submitted for data extraction and analysis. These studies were published between 1999 and 2016 and, collectively, enrolled 3063 FDRs. Positive and negative likelihood ratios (LR+ and LR-, respectively) for CD diagnosis, according to the presence of the HLA-DQ genotype coding a complete MHC-DQ2 and/or MHC-DQ8 molecules, were 1.449 (CI 1.279-1.642) and 0.187 (CI 0.096-0.362), respectively. If only the isolated presence of HLA-DQB1*02 allele is considered, the pooled estimation of LR+ was 1.659 (CI 1.302-2.155) and, importantly, the LR- still showed a very good discriminatory power of 0.195 (CI 0.068-0.558). Conclusions: Through our differential meta-analysis, comparing the presence of the genotype coding the full MHC-DQ2 and/or DQ8 molecules with the isolated presence of HLA-DQB1*02 allelic variant, we found that the LR- of the latter analysis maintained the same value. This observation, along with previous evidences, might be useful to consider potential cost-effective widened screening strategies for CD in children.
2019-12-12T03:19:38Z
2019-12-12T03:19:38Z
2019-05
Article
Capittini, C., De Silvestri, A., Rebuffi, C., Tinelli, C., & Poddighe, D. (2019). Relevance of HLA-DQB1*02 Allele in the Genetic Predisposition of Children with Celiac Disease: Additional Cues from a Meta-Analysis. Medicina, 55(5), 190. https://doi.org/10.3390/medicina55050190
10.3390/medicina55050190
http://nur.nu.edu.kz/handle/123456789/4398
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
MDPI
oai:nur.nu.edu.kz:123456789/43992019-12-12T21:01:22Zcom_123456789_104com_123456789_67col_123456789_105
Human Papillomavirus Infection and Cervical Cancer: Epidemiology, Screening, and Vaccination-Review of Current Perspectives
Chan, Chee-Kai
Aimagambetova, Gulzhanat
Ukybassova, Talshyn
Kongrtay, Kuralay
Azizan, Azliyati
Viral infections
carcinogenesis
cancer
HPV genotypes
HPV
https://www.hindawi.com/journals/jo/2019/3257939/
Viral infections contribute as a cause of 15–20% of all human cancers. Infection by oncogenic viruses can promote different stages of carcinogenesis. Among many types of HPV, around 15 are linked to cancer. In spite of effective screening methods, cervical cancer continues to be a major public health problem. There are wide differences in cervical cancer incidence and mortality by geographic region. In addition, the age-specific HPV prevalence varies widely across different populations and showed two peaks of HPV positivity in younger and older women. There have been many studies worldwide on the epidemiology of HPV infection and oncogenic properties due to different HPV genotypes. However, there are still many countries where the population-based prevalence has not yet been identified. Moreover, cervical cancer screening strategies are different between countries. Organized cervical screening programs are potentially more effective than opportunistic screening programs. Nevertheless, screening programs have consistently been associated with a reduction in cervical cancer incidence and mortality. Developed countries have achieved such reduced incidence and mortality from cervical cancer over the past 40 years. This is largely due to the implementation of organized cytological screening and vaccination programs. HPV vaccines are very effective at preventing infection and diseases related to the vaccine-specific genotypes in women with no evidence of past or current HPV infection. In spite of the successful implementation of the HPV vaccination program in many countries all over the world, problems related to HPV prevention and treatment of the related diseases will continue to persist in developing and underdeveloped countries.
2019-12-12T03:19:39Z
2019-12-12T03:19:39Z
2019-10
Article
han, C. K., Aimagambetova, G., Ukybassova, T., Kongrtay, K., & Azizan, A. (2019). Human Papillomavirus Infection and Cervical Cancer: Epidemiology, Screening, and Vaccination—Review of Current Perspectives. Journal of Oncology, 2019, 1–11. https://doi.org/10.1155/2019/3257939
10.1155/2019/3257939
http://nur.nu.edu.kz/handle/123456789/4399
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Hindawi
oai:nur.nu.edu.kz:123456789/44052019-12-12T21:02:39Zcom_123456789_104com_123456789_67col_123456789_105
Establishment and Characterization of 5-Fluorouracil-Resistant Human Colorectal Cancer Stem-Like Cells: Tumor Dynamics under Selection Pressure
Francipane, M. G.
Bulanin, Denis
Lagasse, E.
cancer stem cells
chemoresistance
colorectal cancer
https://www.ncbi.nlm.nih.gov/pubmed/31013771
5-Fluorouracil (5-FU) remains the gold standard of first-line treatment for colorectal cancer (CRC). Although it may initially debulk the tumor mass, relapses frequently occur, indicating the existence of cancer cells that are therapy-resistant and are capable of refueling tumor growth. To identify mechanisms of drug resistance, CRC stem-like cells were subjected to long-term 5-FU selection using either intermittent treatment regimen with the IC50 drug dose or continuous treatment regimen with escalating drug doses. Parental cancer cells were cultivated in parallel. Real-time PCR arrays and bioinformatic tools were used to investigate gene expression changes. We found the first method selected for cancer cells with more aggressive features. We therefore transplanted these cancer cells or parental cells in mice, and again, found that not only did the 5-FU-selected cancer cells generate more aggressive tumors with respect to their parental counterpart, but they also showed a different gene expression pattern as compared to what we had observed in vitro, with ID1 the top upregulated gene. We propose ID1 as a stemness marker pervasively expressed in secondary lesions emerging after completion of chemotherapy.
2019-12-12T04:07:17Z
2019-12-12T04:07:17Z
2019-04-12
Article
Francipane, M. G., Bulanin, D., & Lagasse, E. (2019). Establishment and Characterization of 5-Fluorouracil-Resistant Human Colorectal Cancer Stem-Like Cells: Tumor Dynamics under Selection Pressure. International Journal of Molecular Sciences, 20(8), 1817. https://doi.org/10.3390/ijms20081817
10.3390/ijms20081817
http://nur.nu.edu.kz/handle/123456789/4405
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
MDPI
oai:nur.nu.edu.kz:123456789/44042019-12-12T21:02:46Zcom_123456789_104com_123456789_67col_123456789_105
IKZF1 genetic variants rs4132601 and rs11978267 and acute lymphoblastic leukemia risk in Tunisian children: a casecontrol study
Sana, Mahjoub
Vera, Chayeb
Hedia, Zitouni
Rabeb M., Ghali
Haifa, Regaieg
Wassim Y., Almawi
Touhami, Mahjoub
Acute lymphoblastic leukemia
Gene polymorphism
IKZF1, rs4132601
rs111978267
Background
Associations between IKZF1 gene variants and Acute Lymphoblastic Leukemia (ALL) was recently reported. We examined whether the common IKZF1 polymorphisms rs4132601 T/G and rs111978267 A/G are associated with ALL among a Tunisian pediatric cohort.
Methods
This case-control study involved 170 patients with ALL and 150 control subjects. SNP genotyping was performed by TaqMan® SNP Genotyping Assay.
2019-12-12T04:07:05Z
2019-12-12T04:07:05Z
2019-10-11
Article
Mahjoub, S., Chayeb, V., Zitouni, H. et al. IKZF1 genetic variants rs4132601 and rs11978267 and acute lymphoblastic leukemia risk in Tunisian children: a case-control study. BMC Med Genet 20, 159 (2019) doi:10.1186/s12881-019-0900-1
http://nur.nu.edu.kz/handle/123456789/4404
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
BMC MEDICAL GENETICS
oai:nur.nu.edu.kz:123456789/44282019-12-12T21:01:57Zcom_123456789_104com_123456789_67col_123456789_105
Maternal regulation of chromosomal imprinting in animals
Singh, Prim B.
Shloma, Victor V.
Belyakin, Stepan N.
Chromosomal imprinting requires an epigenetic system that imprints one of the two parental chromosomes such that it results in a heritable (cell-to-cell) change in behavior of the imprinted chromosome. Imprinting takes place when the parental genomes are separate, which occurs during gamete formation in the respective germ-lines and post-fertilization during the period when the parental pro-nuclei lie separately within the ooplasm of the zygote. In the mouse, chromosomal imprinting is regulated by germ-line specific DNA methylation. But the methylation machinery in the respective germ-lines does not discriminate between imprinted and non-imprinted regions. As a consequence, the mouse oocyte nucleus contains over a thousand oocyte-specific germ-line differentially methylated regions (gDMRs). Upon fertilization, the sperm provides a few hundred sperm-specific gDMRs of its own. Combined, there are around 1600 imprinted and non-imprinted gDMRs in the pro-nuclei of the newly fertilized zygote. It is a remarkable fact that beginning in the maternal ooplasm, there are mechanisms that manage to preserve DNA methylation at 26 known imprinted gDMRs in the face of the ongoing genome-wide DNA de-methylation that characterizes pre-implantation development. Specificity is achieved through the binding of KRAB-zinc finger proteins to their cognate recognition sequences within the gDMRs of imprinted genes. This in turn nucleates the assembly of localized heterochromatin-like complexes that preserve methylation at imprinted gDMRs through recruitment of the maintenance methyl transferase Dnmt1. These studies have shown that a germ-line imprint may cause parent-of-origin-specific behavior only if licensed by mechanisms that operate post-fertilization. Study of the germ-line and post-fertilization contributions to the imprinting of chromosomes in classical insect systems (Coccidae and Sciaridae) show that the ooplasm is the likely site where imprinting takes place. By comparing molecular and genetic studies across these three species, we suggest that mechanisms which operate post-fertilization play a key role in chromosomal imprinting phenomena in animals and conserved components of heterochromatin are shared by these mechanisms.
2019-12-12T08:08:18Z
2019-12-12T08:08:18Z
2019-06
Article
Singh, P. B., Shloma, V. V., & Belyakin, S. N. (2019). Maternal regulation of chromosomal imprinting in animals. Chromosoma, 1-12.
http://nur.nu.edu.kz/handle/123456789/4428
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
SPRINGER
oai:nur.nu.edu.kz:123456789/44602019-12-18T21:00:39Zcom_123456789_104com_123456789_67col_123456789_105
Pruritus in patients with amyopathic dermatomyositis
Poddighe, Dimitri
Mukusheva, Zaure
Assylbekova, Maikesh
Amyopathic dermatomyositis has been associated with the exposure to several drugs: the article by Jeimy et al. described the onset of this uncommon disease in a patient treated with omalizumab. Paradoxically, this patient complained of an intense pruritus and this finding has been reported by several authors observing patients with amyopathic dermatomyositis.
2019-12-18T05:07:52Z
2019-12-18T05:07:52Z
2019-03-27
Article
Poddighe, D., Mukusheva, Z. & Assylbekova, M. Pruritus in patients with amyopathic dermatomyositis. Allergy Asthma Clin Immunol 15, 18 (2019) doi:10.1186/s13223-019-0334-5
https://doi.org/10.1186/s13223-019-0334-5
http://nur.nu.edu.kz/handle/123456789/4460
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
BMC
oai:nur.nu.edu.kz:123456789/44612019-12-18T21:00:36Zcom_123456789_104com_123456789_67col_123456789_105
The prevalence of chronic spontaneous urticaria (CSU) in the pediatric population
Poddighe, Dimitri
2019-12-18T05:24:17Z
2019-12-18T05:24:17Z
2019-11
Article
Poddighe, D. (2019). The prevalence of chronic spontaneous urticaria (CSU) in the pediatric population. Journal of the American Academy of Dermatology, 81(5), e149.
https://doi.org/10.1016/j.jaad.2019.07.068
http://nur.nu.edu.kz/handle/123456789/4461
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
MOSBY-ELSEVIER
oai:nur.nu.edu.kz:123456789/44982020-02-19T21:00:21Zcom_123456789_104com_123456789_67col_123456789_105
Late Diagnosis of Post-partum Colorectal Cancer in a Young Kazakh Woman
Dauyey, Kaisar
Jumadilova, Dinara
colorectal cancer
post-partum
young age
Research Subject Categories::MEDICINE
http://www.ivyunion.org/index.php/ajccr/article/view/1343
Introduction: Colorectal cancer (CRC) is generally expected among population above 60 years of age and rarely occurs in younger age groups. Its occurrence in young individuals is sporadic and has poor prognosis.
Presentation of the case: A 24-year old woman, previously healthy, delivered her first child and subsequently developed pelvic problems. Despite multiple surgeries and diagnostic work-up, which included cross-sectional imaging, the patient’s diagnosis remained unknown. At the time of diagnosis with a colonoscopic evaluation, the patient was in the late stage of disease and died few months later.
Conclusion: CRC should be in the differentials list in all patient cases, when suggestive symptoms present, regardless of age and other factors. Its association with pregnancy may obscure symptoms and delay the diagnosis.
2020-02-19T04:57:21Z
2020-02-19T04:57:21Z
2020
Article
Dauyey, K., & Jumadilova, D. (2020). Late Diagnosis of Post-partum Colorectal Cancer in a Young Kazakh Woman. 8(1), 1–5.
2572-5742
http://www.ivyunion.org/index.php/ajccr/article/view/1343
http://nur.nu.edu.kz/handle/123456789/4498
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Ivy Union Publishing
oai:nur.nu.edu.kz:123456789/45502020-03-26T21:00:49Zcom_123456789_104com_123456789_67col_123456789_105
Deletion of HP1γ in cardiac myocytes afects H4K20me3 levels but does not impact cardiac growth
El‑Nachef, Danny
Fang, Chen
Kajimoto, Hidemi
Brown, Jeremy P.
Singh, Prim B.
MacLellan, W. Robb
cardiac myocytes
cell cycle
conditional knockout
gene expression
Heterochromatin, which is formed when tri-methyl lysine 9 of histone H3 (H3K9me3) is bound by heterochromatin 1 proteins (HP1s), plays an important role in differentiation and senescence by silencing cell cycle genes. Cardiac myocytes (CMs) accumulate heterochromatin during differentiation and demethylation of H3K9me3 inhibits cell cycle gene silencing and cell cycle exit in CMs; however, it is unclear if this process is mediated by HP1s. In this study, we created a conditional CM-specific HP1 gamma (HP1γ) knockout (KO) mouse model and tested whether HP1γ is required for cell cycle gene silencing and cardiac growth....
2020-03-26T11:12:25Z
2020-03-26T11:12:25Z
2018-04-18
Article
Oyama K., El-Nachef D., Fang C., Kajimoto H., Brown JP., Singh PB., MacLellan WR. (2018) Deletion of HP1γ in cardiac myocytes affects H4K20me3 levels but does not impact cardiac growth.Epigenetics Chromatin.
http://nur.nu.edu.kz/handle/123456789/4550
en
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Epigenetics Chromatin
oai:nur.nu.edu.kz:123456789/45532020-03-27T21:00:37Zcom_123456789_104com_123456789_67col_123456789_105
Rational CCL5 mutagenesis integration in a lactobacilli platform generates extremely potent HIV-1 blockers
Lanzafame, Massimiliano
Grampa, Valentina
Vangelista, Luca
CCL5
mutagenes
HIV-1 blockers
Research Subject Categories::MEDICINE
lactobacilli
https://www.nature.com/articles/s41598-018-20300-9
Efforts to improve existing anti-HIV-1 therapies or develop preventatives have identified CCR5 as an important target and CCL5 as an ideal scaffold to sculpt potent HIV-1 entry inhibitors. We created novel human CCL5 variants that exhibit exceptional anti-HIV-1 features using recombinant lactobacilli (exploited for live microbicide development) as a screening platform. Protein design, expression and anti-HIV-1 activity flowed in iterative cycles, with a stepwise integration of successful mutations and refinement of an initial CCL5 mutant battery towards the generation of two ultimate CCL5 derivatives, a CCR5 agonist and a CCR5 antagonist with similar anti-HIV-1 potency. The CCR5 antagonist was tested in human macrophages and against primary R5 HIV-1 strains, exhibiting cross-clade low picomolar IC50 activity. Moreover, its successful combination with several HIV-1 inhibitors provided the ground for conceiving therapeutic and preventative anti-HIV-1 cocktails. Beyond HIV-1 infection, these CCL5 derivatives may now be tested against several inflammation-related pathologies where the CCL5:CCR5 axis plays a relevant role.
2020-03-27T05:35:32Z
2020-03-27T05:35:32Z
2018
Article
Secchi, M., Grampa, V., & Vangelista, L. (2018). Rational CCL5 mutagenesis integration in a lactobacilli platform generates extremely potent HIV-1 blockers. Scientific Reports, 8(1). https://doi.org/10.1038/s41598-018-20300-9
2045-2322
10.1038/s41598-018-20300-9
https://www.nature.com/articles/s41598-018-20300-9.pdf
http://nur.nu.edu.kz/handle/123456789/4553
en
Scientific Reports;
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Nature Research
oai:nur.nu.edu.kz:123456789/45542020-03-27T21:00:20Zcom_123456789_104com_123456789_67col_123456789_105
A homozygous missense variant in VWA2, encoding an interactor of the Fraser-complex, in a patient with vesicoureteral refluxy
T. van der Ven, Amelie
Kobbe, Birgit
Kohl, Stefan
Shril, Shirlee
Pogoda, Hans-Martin
Imhof, Thomas
Ityel, Hadas
Vivante, Asaf
Chen, Jing
Hwang, Daw-Yang
M. Connaughton, Dervla
Mann, Nina
Widmeier, Eugen
Taglienti, Mary
Schmidt, Johanna Magdalena
Nakayama, Makiko
Senguttuvan, Prabha
Kumar, Selvin
Tasic, Velibor
Kehinde, Elijah O.
M. Mane, Shrikant
P. Lifton, Richard
Soliman, Neveen
Lu, Weining
B. Bauer, Stuart
Hammerschmidt, Matthias
Wagener, Raimund
Hildebrandt, Friedhelm
Research Subject Categories::MEDICINE
chronic kidney disease
CKD
congenital anomalies of the kidney and urinary tract
CAKUT
whole exome sequencing
WES
VWA2
ureteric bud
UB
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0191224
Congenital anomalies of the kidney and urinary tract (CAKUT) are the most common cause (40–50%) of chronic kidney disease (CKD) in children. About 40 monogenic causes of CAKUT have so far been discovered. To date less than 20% of CAKUT cases can be explained by mutations in these 40 genes. To identify additional monogenic causes of CAKUT, we performed whole exome sequencing (WES) and homozygosity mapping (HM) in a patient with CAKUT from Indian origin and consanguineous descent. We identified a homozygous missense mutation (c.1336C>T, p.Arg446Cys) in the gene Von Willebrand factor A domain containing 2 (VWA2). With immunohistochemistry studies on kidneys of newborn (P1) mice, we show that Vwa2 and Fraser extracellular matrix complex subunit 1 (Fras1) co-localize in the nephrogenic zone of the renal cortex. We identified a pronounced expression of Vwa2 in the basement membrane of the ureteric bud (UB) and derivatives of the metanephric mesenchyme (MM). By applying in vitro assays, we demonstrate that the Arg446Cys mutation decreases translocation of monomeric VWA2 protein and increases translocation of aggregated VWA2 protein into the extracellular space. This is potentially due to the additional, unpaired cysteine residue in the mutated protein that is used for intermolecular disulfide bond formation. VWA2 is a known, direct interactor of FRAS1 of the Fraser-Complex (FC). FC-encoding genes and interacting proteins have previously been implicated in the pathogenesis of syndromic and/or isolated CAKUT phenotypes in humans. VWA2 therefore constitutes a very strong candidate in the search for novel CAKUT-causing genes. Our results from in vitro experiments indicate a dose-dependent neomorphic effect of the Arg446Cys homozygous mutation in VWA2.
2020-03-27T05:35:35Z
2020-03-27T05:35:35Z
2018-01
Article
van der Ven, A. T., Kobbe, B., Kohl, S., Shril, S., Pogoda, H.-M., Imhof, T., Ityel, H., Vivante, A., Chen, J., Hwang, D.-Y., Connaughton, D. M., Mann, N., Widmeier, E., Taglienti, M., Schmidt, J. M., Nakayama, M., Senguttuvan, P., Kumar, S., Tasic, V., … Hildebrandt, F. (2018). A homozygous missense variant in VWA2, encoding an interactor of the Fraser-complex, in a patient with vesicoureteral reflux. PLOS ONE, 13(1), e0191224. https://doi.org/10.1371/journal.pone.0191224
1932-6203
10.1371/journal.pone.0191224
https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0191224&type=printable
http://nur.nu.edu.kz/handle/123456789/4554
en
PLoS ONE;
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
Public Library of Science
oai:nur.nu.edu.kz:123456789/45582020-03-30T21:00:34Zcom_123456789_104com_123456789_67col_123456789_105
Detection of Hepatitis E Antibodies in Kazakhstan: A Pilot Study
Cainelli, Francesca
Hortelano, Gonzalo
Negmetzhanov, B.
Ibrayeva, A.
Kaliaskarova, Kulpash
Bulanin, Denis
Vento, Sandro
Hepatitis E
anti-HEV antibodies
Kazakhstan
Research Subject Categories::MEDICINE
http://cajgh.pitt.edu/ojs/index.php/cajgh/article/view/324
Introduction. Hepatitis E virus exposure is associated with sporadic cases of acute hepatitis and outbreaks in many countries worldwide. It is particularly dangerous for pregnant women, in whom the mortality rate is high. There are no previously published data reporting circulation of this virus in Kazakhstan.
Methods. We tested blood samples for IgG anti-hepatitis E virus antibodies in 199 Kazakh participants; of these 119 were workers at the EXPO 2017 building site in Astana, 35 were volunteers who got tested at the Astana City Hall on the World Hepatitis Day 2017, and 45 were volunteers who presented for screening at the Hepatogastroenterology Outpatient Clinic of the Republican Diagnostic Center, University Medical Center.
Results. 11 (5.5%) individuals were positive for IgG anti-HEV antibodies, with a higher seroprevalence in males (7; 6.8%) vs females (4; 4.5%). The highest number of positive samples was in the 32-46 years age group.
Conclusions. This pilot study suggests that Hepatitis E virus has been circulating in Kazakhstan. Studies are needed to determine whether it continues to be present, which viral genotypes are involved and what are the best methodologies for preventing its spread.
2020-03-30T04:16:53Z
2020-03-30T04:16:53Z
2018
Article
Cainelli, F., Hortelano, G., Negmetzhanov, B., Ibrayeva, A., Kaliaskarova, K., Bulanin, D., & Vento, S. (2018). Detection of Hepatitis E Antibodies in Kazakhstan: A Pilot Study. Central Asian Journal of Global Health, 7(1). https://doi.org/10.5195/cajgh.2018.324
2166-7403
10.5195/cajgh.2018.324
http://cajgh.pitt.edu/ojs/index.php/cajgh/article/view/324/252
http://nur.nu.edu.kz/handle/123456789/4558
en
Central Asian Journal of Global Health;Vol 7, No 1 (2018)
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
University of Pittsburgh, University Library System
oai:nur.nu.edu.kz:123456789/45562020-03-30T21:00:34Zcom_123456789_104com_123456789_67col_123456789_105
Discovery of Actinomycetes from Extreme Environments with Potential to Produce Novel Antibiotics
Trenozhnikova, Lyudmila
Azizan, Azliyati
actinomycetes
antibiotics
pathogens
antibiotic resistance
pathogens
natural products
extremophiles
http://cajgh.pitt.edu/ojs/index.php/cajgh/article/view/337
Introduction: Antimicrobial-resistant pathogens pose serious challenges to healthcare institutions and health of the public. Thus, there is an urgent need for the discovery of new and effective antimicrobial agents. Microorganisms that exist in extreme environments such as those with high salinity or alkalinity, are known as extremophiles, and include various species of actinomycetes. The goal of this study is to discover novel antibiotics from extremophiles found in Kazakhstan that are effective against drug resistant pathogens.
Methods: Soil from extreme environments of Kazakhstan was collected, and pure cultures of actinomycetes were isolated and cultured in modified Bennett’s broth with either high concentrations of salt or high pH to mimic extreme environments. Extracts obtained from selected actinomycetes strains were used to test for antimicrobial activity against Staphylococcus aureus, Escherichia coli and Aspergillus niger.
Results: A total of 5936 strains of extremophile actinomycetes were isolated; from these, 2019 strains were further isolated into pure cultures. Of these, 415 actinomycetes strains that demonstrated antagonistic antibacterial activities were selected. These actinomycetes were further classified into groups and subgroups based on their responses to different culture conditions. Antimicrobial antagonism activity for some of the actinomycetes strains was dependent on culture conditions and development of aerial mycelia under extreme conditions.
Conclusion: We identified several interesting candidate extracts with putative antimicrobial activities against several strains of drug resistant pathogens. Our research of the actinomycetes’ ability to produce antibiotics in the near-natural conditions provides a great opportunity to assess their biodiversity and distribution in the Central Asian region and to develop new methodological approaches to the screening of new antimicrobial agents.
2020-03-30T04:16:13Z
2020-03-30T04:16:13Z
2018
Article
Trenozhnikova, L., & Azizan, A. (2018). Discovery of Actinomycetes from Extreme Environments with Potential to Produce Novel Antibiotics. Central Asian Journal of Global Health, 7(1). https://doi.org/10.5195/cajgh.2018.337
2166-7403
10.5195/cajgh.2018.337
http://cajgh.pitt.edu/ojs/index.php/cajgh/article/view/337/262
http://nur.nu.edu.kz/handle/123456789/4556
en
Central Asian Journal of Global Health;Vol 7, No 1 (2018)
http://creativecommons.org/licenses/by-nc-sa/3.0/us/
Attribution-NonCommercial-ShareAlike 3.0 United States
University of Pittsburgh, University Library System
rdf///col_123456789_105/100